Skip to main content
Erschienen in: Infection 6/2012

01.12.2012 | Case Report

Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever

verfasst von: D. Limonta, V. Falcón, G. Torres, V. Capó, I. Menéndez, D. Rosario, Y. Castellanos, M. Alvarez, R. Rodríguez-Roche, M. C. de la Rosa, A. Pavón, L. López, K. González, G. Guillén, J. Diaz, M. G. Guzmán

Erschienen in: Infection | Ausgabe 6/2012

Einloggen, um Zugang zu erhalten

Abstract

Dengue virus is the most significant virus transmitted by arthropods worldwide and may cause a potentially fatal systemic disease named dengue hemorrhagic fever. In this work, dengue virus serotype 4 was detected in the tissues of one fatal dengue hemorrhagic fever case using electron immunomicroscopy and molecular methods. This is the first report of dengue virus polypeptides findings by electron immunomicroscopy in human samples. In addition, not-previously-documented virus-like particles visualized in spleen, hepatic, brain, and pulmonary tissues from a dengue case are discussed.
Literatur
2.
Zurück zum Zitat San Martín JL, Brathwaite O, Zambrano B, Solórzano JO, Bouckenooghe A, Dayan GH, et al. The epidemiology of dengue in the Americas over the last three decades: a worrisome reality. Am J Trop Med Hyg. 2010;82:128–35. doi:10.4269/ajtmh.2010.09-0346.PubMedCrossRef San Martín JL, Brathwaite O, Zambrano B, Solórzano JO, Bouckenooghe A, Dayan GH, et al. The epidemiology of dengue in the Americas over the last three decades: a worrisome reality. Am J Trop Med Hyg. 2010;82:128–35. doi:10.​4269/​ajtmh.​2010.​09-0346.PubMedCrossRef
3.
Zurück zum Zitat World Health Organization (WHO). Dengue: guidelines for diagnosis, treatment, prevention and control. WHO. 2009. World Health Organization (WHO). Dengue: guidelines for diagnosis, treatment, prevention and control. WHO. 2009.
4.
Zurück zum Zitat Kouri GP, Guzmán MG, Bravo JR. Why dengue haemorrhagic fever in Cuba? 2. An integral analysis. Trans R Soc Trop Med Hyg. 1987;81:821–3.PubMedCrossRef Kouri GP, Guzmán MG, Bravo JR. Why dengue haemorrhagic fever in Cuba? 2. An integral analysis. Trans R Soc Trop Med Hyg. 1987;81:821–3.PubMedCrossRef
5.
Zurück zum Zitat Guzmán MG, Kouri G, Valdes L, Bravo J, Alvarez M, Vazques S, et al. Epidemiologic studies on Dengue in Santiago de Cuba, 1997. Am J Epidemiol. 2000;152:793–9.PubMedCrossRef Guzmán MG, Kouri G, Valdes L, Bravo J, Alvarez M, Vazques S, et al. Epidemiologic studies on Dengue in Santiago de Cuba, 1997. Am J Epidemiol. 2000;152:793–9.PubMedCrossRef
7.
Zurück zum Zitat Peláez O, Guzmán MG, Kourí G, Pérez R, San Martín JL, Vázquez S, et al. Dengue 3 epidemic, Havana, 2001. Emerg Infect Dis. 2004;10:719–22.PubMedCrossRef Peláez O, Guzmán MG, Kourí G, Pérez R, San Martín JL, Vázquez S, et al. Dengue 3 epidemic, Havana, 2001. Emerg Infect Dis. 2004;10:719–22.PubMedCrossRef
8.
Zurück zum Zitat OPS-Cuba. Brote de dengue en Cuba, 2006. EER Noticias: Enfermedades infecciosas emergentes y reemergentes, Región de Las Américas. 2006;3. OPS-Cuba. Brote de dengue en Cuba, 2006. EER Noticias: Enfermedades infecciosas emergentes y reemergentes, Región de Las Américas. 2006;3.
9.
Zurück zum Zitat García G, González N, Pérez AB, Sierra B, Aguirre E, Rizo D, et al. Long-term persistence of clinical symptoms in dengue-infected persons and its association with immunological disorders. Int J Infect Dis. 2011;15:e38–43.PubMedCrossRef García G, González N, Pérez AB, Sierra B, Aguirre E, Rizo D, et al. Long-term persistence of clinical symptoms in dengue-infected persons and its association with immunological disorders. Int J Infect Dis. 2011;15:e38–43.PubMedCrossRef
10.
Zurück zum Zitat Guzmán MG. Treinta años después de la epidemia cubana de dengue hemorrágico en 1981. Rev Cub Med Trop. 2012;64:5–14. Guzmán MG. Treinta años después de la epidemia cubana de dengue hemorrágico en 1981. Rev Cub Med Trop. 2012;64:5–14.
11.
Zurück zum Zitat Pelegrino JL, Arteaga E, Rodríguez AJ, González E, Frontela MD, Guzmán MG. Normalizacion de tecnicas inmunohistoquimicas para la detección de antigenos del virus dengue en tejidos embebidos en parafina. Rev Cub Med Trop. 1997;49:100–7. Pelegrino JL, Arteaga E, Rodríguez AJ, González E, Frontela MD, Guzmán MG. Normalizacion de tecnicas inmunohistoquimicas para la detección de antigenos del virus dengue en tejidos embebidos en parafina. Rev Cub Med Trop. 1997;49:100–7.
12.
Zurück zum Zitat Limonta D, Capó V, Torres G, Pérez AB, Guzmán MG. Apoptosis in tissues from fatal dengue shock syndrome. J Clin Virol. 2007;40:50–4.PubMedCrossRef Limonta D, Capó V, Torres G, Pérez AB, Guzmán MG. Apoptosis in tissues from fatal dengue shock syndrome. J Clin Virol. 2007;40:50–4.PubMedCrossRef
13.
Zurück zum Zitat Rodriguez-Roche R, Alvarez M, Guzmán MG, Morier L, Kourí G. Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells. J Clin Microbiol. 2000;38:3508–10. Rodriguez-Roche R, Alvarez M, Guzmán MG, Morier L, Kourí G. Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells. J Clin Microbiol. 2000;38:3508–10.
14.
Zurück zum Zitat Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol. 1992;30:545–51.PubMed Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol. 1992;30:545–51.PubMed
15.
Zurück zum Zitat Rosario D, Álvarez M, Vázquez S, Amin N, Rodríguez-Roche R, Guzmán MG. Application of molecular methods to the diagnosis and characterization of a dengue outbreak in Cuba. Biotecnologia Aplicada. 2001;18:203–6. Rosario D, Álvarez M, Vázquez S, Amin N, Rodríguez-Roche R, Guzmán MG. Application of molecular methods to the diagnosis and characterization of a dengue outbreak in Cuba. Biotecnologia Aplicada. 2001;18:203–6.
16.
Zurück zum Zitat Callahan JD, Wu SJ, Dion-Schultz A, Mangold BE, Peruski LF, Watts DM, et al. Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus. J Clin Microbiol. 2001;39:4119–24.PubMedCrossRef Callahan JD, Wu SJ, Dion-Schultz A, Mangold BE, Peruski LF, Watts DM, et al. Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus. J Clin Microbiol. 2001;39:4119–24.PubMedCrossRef
17.
Zurück zum Zitat Faulk WP, Taylor GM. An immunocolloid method for the electron microscope. Immunochemistry. 1971;8:1081–3.PubMedCrossRef Faulk WP, Taylor GM. An immunocolloid method for the electron microscope. Immunochemistry. 1971;8:1081–3.PubMedCrossRef
18.
Zurück zum Zitat Kellenberger E, Dürrenberger M, Villiger W, Carlemalm E, Wurtz M. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J Histochem Cytochem. 1987;35:959–69.PubMedCrossRef Kellenberger E, Dürrenberger M, Villiger W, Carlemalm E, Wurtz M. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J Histochem Cytochem. 1987;35:959–69.PubMedCrossRef
19.
Zurück zum Zitat Hermida Díaz C, Pupo M, Guzmán Tirado MG, González Garriga M, Marcet Sánchez R. Empleo de un anticuerpo monoclonal anticomplejo dengue en la purificacion viral. Rev Cub Med Trop. 1992;44:171–6. Hermida Díaz C, Pupo M, Guzmán Tirado MG, González Garriga M, Marcet Sánchez R. Empleo de un anticuerpo monoclonal anticomplejo dengue en la purificacion viral. Rev Cub Med Trop. 1992;44:171–6.
20.
Zurück zum Zitat Fernández de Cossío ME, Díaz T, Galván A, Valdéz R, González E, Ayala M et al. Antigen recognition characteristics and comparative performance in immunoaffinity purification of two monoclonal antibodies specific for the hepatitis B virus surface antigen. J Biotechnol. 1997;56:69–80.PubMedCrossRef Fernández de Cossío ME, Díaz T, Galván A, Valdéz R, González E, Ayala M et al. Antigen recognition characteristics and comparative performance in immunoaffinity purification of two monoclonal antibodies specific for the hepatitis B virus surface antigen. J Biotechnol. 1997;56:69–80.PubMedCrossRef
21.
Zurück zum Zitat Guzmán MG, Alvarez M, Rodríguez R, Rosario D, Vázquez S, Valdés L, et al. Fatal dengue hemorrhagic fever in Cuba, 1997. Int J Infect Dis. 1999;3:130–5.PubMedCrossRef Guzmán MG, Alvarez M, Rodríguez R, Rosario D, Vázquez S, Valdés L, et al. Fatal dengue hemorrhagic fever in Cuba, 1997. Int J Infect Dis. 1999;3:130–5.PubMedCrossRef
22.
Zurück zum Zitat Pan American Health Organization (PAHO). Dengue and dengue hemorrhagic fever in the Americas: guidelines for prevention and control. Washington: PAHO. 1994. Pan American Health Organization (PAHO). Dengue and dengue hemorrhagic fever in the Americas: guidelines for prevention and control. Washington: PAHO. 1994.
23.
Zurück zum Zitat Bhamarapravati N, Tuchinda P, Boonyapaknavik V. Pathology of Thailand haemorrhagic fever: a study of 100 autopsy cases. Ann Trop Med Parasitol. 1967;61:500–10.PubMed Bhamarapravati N, Tuchinda P, Boonyapaknavik V. Pathology of Thailand haemorrhagic fever: a study of 100 autopsy cases. Ann Trop Med Parasitol. 1967;61:500–10.PubMed
24.
Zurück zum Zitat Srikiatkhachorn A, Krautrachue A, Ratanaprakarn W, Wongtapradit L, Nithipanya N, Kalayanarooj S, et al. Natural history of plasma leakage in dengue hemorrhagic fever: a serial ultrasonographic study. Pediatr Infect Dis J. 2007;26:283–90.PubMedCrossRef Srikiatkhachorn A, Krautrachue A, Ratanaprakarn W, Wongtapradit L, Nithipanya N, Kalayanarooj S, et al. Natural history of plasma leakage in dengue hemorrhagic fever: a serial ultrasonographic study. Pediatr Infect Dis J. 2007;26:283–90.PubMedCrossRef
25.
Zurück zum Zitat Schmidt-Chanasit J, Tenner-Racz K, Poppert D, Emmerich P, Frank C, Dinges C, et al. Fatal dengue hemorrhagic fever imported into Germany. Infection. 2011. doi:10.1007/s15010-011-0208-3. Schmidt-Chanasit J, Tenner-Racz K, Poppert D, Emmerich P, Frank C, Dinges C, et al. Fatal dengue hemorrhagic fever imported into Germany. Infection. 2011. doi:10.​1007/​s15010-011-0208-3.
26.
Zurück zum Zitat Limonta D, González D, Capó V, Torres G, Pérez AB, Rosario D, et al. Fatal severe dengue and cell death in sickle cell disease during the 2001–2002 Havana dengue epidemic. Int J Infect Dis. 2009;13:e77–8.PubMedCrossRef Limonta D, González D, Capó V, Torres G, Pérez AB, Rosario D, et al. Fatal severe dengue and cell death in sickle cell disease during the 2001–2002 Havana dengue epidemic. Int J Infect Dis. 2009;13:e77–8.PubMedCrossRef
27.
Zurück zum Zitat de Araújo JM, Schatzmayr HG, de Filippis AM, Dos Santos FB, Cardoso MA, Britto C, et al. A retrospective survey of dengue virus infection in fatal cases from an epidemic in Brazil. J Virol Methods. 2009;155:34–8.PubMedCrossRef de Araújo JM, Schatzmayr HG, de Filippis AM, Dos Santos FB, Cardoso MA, Britto C, et al. A retrospective survey of dengue virus infection in fatal cases from an epidemic in Brazil. J Virol Methods. 2009;155:34–8.PubMedCrossRef
28.
Zurück zum Zitat Boonpucknavig V, Bhamarapravati N, Boonpucknavig S, Futrakul P, Tanpaichitr P. Glomerular changes in dengue hemorrhagic fever. Arch Pathol Lab Med. 1976;100:206–12.PubMed Boonpucknavig V, Bhamarapravati N, Boonpucknavig S, Futrakul P, Tanpaichitr P. Glomerular changes in dengue hemorrhagic fever. Arch Pathol Lab Med. 1976;100:206–12.PubMed
29.
Zurück zum Zitat Allwinn R, Schieferstein C, Glauke S, Doerr HW. Rapid diagnosis of primary dengue fever by the immunochromatographic test and by electron microscopy—a case report. Infection. 1999;27:365–7.PubMedCrossRef Allwinn R, Schieferstein C, Glauke S, Doerr HW. Rapid diagnosis of primary dengue fever by the immunochromatographic test and by electron microscopy—a case report. Infection. 1999;27:365–7.PubMedCrossRef
30.
Zurück zum Zitat Noisakran S, Gibbons RV, Songprakhon P, Jairungsri A, Ajariyakhajorn C, Nisalak A, et al. Detection of dengue virus in platelets isolated from dengue patients. Southeast Asian J Trop Med Public Health. 2009;40:253–62.PubMed Noisakran S, Gibbons RV, Songprakhon P, Jairungsri A, Ajariyakhajorn C, Nisalak A, et al. Detection of dengue virus in platelets isolated from dengue patients. Southeast Asian J Trop Med Public Health. 2009;40:253–62.PubMed
31.
Zurück zum Zitat Wiersinga WJ, Scheepstra CG, Kasanardjo JS, de Vries PJ, Zaaijer H, Geerlings SE. Dengue fever-induced hemolytic uremic syndrome. Clin Infect Dis. 2006;43:800–1.PubMedCrossRef Wiersinga WJ, Scheepstra CG, Kasanardjo JS, de Vries PJ, Zaaijer H, Geerlings SE. Dengue fever-induced hemolytic uremic syndrome. Clin Infect Dis. 2006;43:800–1.PubMedCrossRef
33.
Zurück zum Zitat Hazelton PR, Gelderblom HR. Electron microscopy for rapid diagnosis of infectious agents in emergent situations. Emerg Infect Dis. 2003;9:294–303.PubMedCrossRef Hazelton PR, Gelderblom HR. Electron microscopy for rapid diagnosis of infectious agents in emergent situations. Emerg Infect Dis. 2003;9:294–303.PubMedCrossRef
35.
Zurück zum Zitat Kulesh DA, Loveless BM, Norwood D, Garrison J, Whitehouse CA, Hartmann C, et al. Monkeypox virus detection in rodents using real-time 3′-minor groove binder TaqMan assays on the Roche LightCycler. Lab Invest. 2004;84:1200–8. doi:10.1038/labinvest.3700143.PubMedCrossRef Kulesh DA, Loveless BM, Norwood D, Garrison J, Whitehouse CA, Hartmann C, et al. Monkeypox virus detection in rodents using real-time 3′-minor groove binder TaqMan assays on the Roche LightCycler. Lab Invest. 2004;84:1200–8. doi:10.​1038/​labinvest.​3700143.PubMedCrossRef
36.
Zurück zum Zitat Jessie K, Fong MY, Devi S, Lam SK, Wong KT. Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization. J Infect Dis. 2004;189:1411–8.PubMedCrossRef Jessie K, Fong MY, Devi S, Lam SK, Wong KT. Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization. J Infect Dis. 2004;189:1411–8.PubMedCrossRef
Metadaten
Titel
Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever
verfasst von
D. Limonta
V. Falcón
G. Torres
V. Capó
I. Menéndez
D. Rosario
Y. Castellanos
M. Alvarez
R. Rodríguez-Roche
M. C. de la Rosa
A. Pavón
L. López
K. González
G. Guillén
J. Diaz
M. G. Guzmán
Publikationsdatum
01.12.2012
Verlag
Springer-Verlag
Erschienen in
Infection / Ausgabe 6/2012
Print ISSN: 0300-8126
Elektronische ISSN: 1439-0973
DOI
https://doi.org/10.1007/s15010-012-0260-7

Weitere Artikel der Ausgabe 6/2012

Infection 6/2012 Zur Ausgabe

Clinical and Epidemiological Study

Hepatitis delta: on soft paws across Germany

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.