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Semin Reprod Med 2002; 20(1): 025-036
DOI: 10.1055/s-2002-23517
Copyright © 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

The Use of Cryopreserved Mature and Immature Testicular Spermatozoa for Intracytoplasmic Sperm Injection: Risks and Limitations

Wolfgang Küpker1 , Safaa Al-Hasani1 , Rainer Johannisson2 , Jörg Sandmann3 , Michael Ludwig1 , Dieter Jocham3 , Klaus Diedrich1
  • 1Department of Obstetrics and Gynecology, Medical University Lübeck, Lübeck, Germany
  • 2Institute of Pathology, Medical University Lübeck, Lübeck, Germany
  • 3Department of Urology, Medical University Lübeck, Lübeck, Germany
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Publication History

Publication Date:
02 April 2002 (online)

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ABSTRACT

Treatment of severe male subfertility has become available since the intracytoplasmic injection of a single sperm into an oocyte was successfully applied for the first time in 1992. Moreover, with the use of fresh and cryopreserved epididymal and testicular spermatozoa for this procedure, fertilization and pregnancies could be accomplished. This review addresses the development and performance of these techniques and discusses achievements and problems as well as future aspects of the feasibility of early spermatid injection. Furthermore, limitations of these procedures and concerns with regard to genetic and epigenetic risks of using immature gametes are stressed.

KEYWORD

TESE - MESA - ROSI - ELSI - cryopreservation - imprinting

REFERENCES

  • 1 Tesarik J. Sperm or spermatid conception?.  Fertil Steril . 1997;  68 214-216
    CrossrefPubMedGoogle Scholar
  • 2 Vanderzwalmen P, Zech H, Birkenfeld A. Intracytoplasmic injection of spermatids retrieved from testicular tissue: influence of testicular pathology, type of selected spermatids and oocyte activation.  Hum Reprod . 1997;  12 1203-1213
    CrossrefPubMedGoogle Scholar
  • 3 Nagy Z, Liu J, Janssenswillen C, Silber S J, Devroey P, Van Steirteghem C A. Using ejaculated, fresh and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection.  Fertil Steril . 1995;  63 808-815
    PubMedGoogle Scholar
  • 4 Schlegel P. Testicular sperm extraction: microdissection improves sperm yield with minimal tissue excision.  Hum Reprod . 1999;  14 131-135
    CrossrefPubMedGoogle Scholar
  • 5 Salzbrunn A, Benson D M, Holstein A F, Schulze W. A new concept for the extraction of testicular spermatozoa as a tool for assisted fertilization (ICSI).  Hum Reprod . 1996;  11 752-755
    PubMedGoogle Scholar
  • 6 Van Steirteghem C A, Nagy Z, Joris H. High fertilization and implantation rates after intracytoplasmatic sperm injection.  Hum Reprod . 1993;  8 1061-1066
    PubMedGoogle Scholar
  • 7 Al-Hasani S, Küpker W, Baschat A A. Mini swim-up: a new technique of sperm preparation for intracytoplasmatic sperm injection.  J Assist Reprod Genet . 1995;  12 428-433
    PubMedGoogle Scholar
  • 8 Johannisson R, Bals-Pratsch M, Diedrich K. Die Bedeutung der Semidünnschnitt-Diagnostik an Hodenbiopsaten bei der Mikroinjektion (ICSI) mit testikulären Spermien (TESE).  Der Frauenarzt . 1998;  39 1398-1404
    PubMedGoogle Scholar
  • 9 Real-Enzyklopädie der gesamten Heilkunde für praktische Ärzte. 1913, Band XIII . 
    Google Scholar
  • 10 Jow W W, Steckel J, Schlegel P N, Magid M S, Goldstein M. Motile sperm in human testis biopsy specimens.  J Androl . 1993;  14 194-198
    PubMedGoogle Scholar
  • 11 Schoysman R, Vanderzwalmen P, Nijs M. Pregnancy after fertilisation with human testicular spermatozoa.  Lancet . 1993;  342 1237
    PubMedGoogle Scholar
  • 12 Schoysman R, Vanderzwalmen P, Nijs M. Pregnancy obtained with human testicular spermatozoa in an in vitro fertilization program.  J Androl . 1994;  15(suppl) 10S-13S
    PubMedGoogle Scholar
  • 13 Devroey P, Liu J, Nagy Z, Tournaye H, Silber S J, Van Steirteghem C A. Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection.  Fertil Steril . 1994;  62 639-641
    CrossrefPubMedGoogle Scholar
  • 14 Devroey P, Liu J, Nagy Z. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia.  Hum Reprod . 1995;  10 101-104
    PubMedGoogle Scholar
  • 15 Kahraman S, Ozgur S, Alatas C. Fertility with testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermic men.  Hum Reprod . 1996;  11 756-760
    CrossrefPubMedGoogle Scholar
  • 16 Madgar I, Seidmann D S, Levran D. Micromanipulation improves in-vitro fertilization results after epididymal or testicular sperm aspiration in patients with congenital absence of the vas deferens.  Hum Reprod . 1996;  11 2151-2154
    PubMedGoogle Scholar
  • 17 Schlegel P N, Palermo G D, Goldstein M. Testicular sperm extraction with intracytoplasmic sperm injection for non-obstructive azoospermia.  Urology . 1997;  49 435-440
    CrossrefPubMedGoogle Scholar
  • 18 Gianaroli L, Magli M C, Selman H A. Diagnostic testicular biopsy and cryopreservation of testicular tissue as an alternative to repeated surgical openings in the treatment of azoospermic men.  Hum Reprod . 1999;  14 1034-1038
    CrossrefPubMedGoogle Scholar
  • 19 Fischer R, Baukloh V, Naether O GJ, Schulze W, Salzbrunn A, Benson D M. Pregnancy after intracytoplasmic sperm injection of spermatozoa extracted from frozen-thawed testicular biopsy.  Hum Reprod . 1996;  11 2197-2199
    PubMedGoogle Scholar
  • 20 Gil-Salom M, Mínguez Y, Rubio C, De los Santos J M, Remohí J, Pellicer A. Efficacy of intracytoplasmic sperm injection using testicular spermatozoa.  Hum Reprod . 1995;  10 3166-3170
    PubMedGoogle Scholar
  • 21 Romero J, Remohí J, Mínguez Y. Fertilization after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa.  Fertil Steril . 1996;  65 877-879
    PubMedGoogle Scholar
  • 22 Perraguin-Jayot S, Audebert A, Emperaire J C, Parneix I. Ongoing pregnancies after introcytoplasmic injection using cryopreserved testicular spermatozoa.  Hum Reprod . 1997;  12 2706-2709
    CrossrefPubMedGoogle Scholar
  • 23 Giwercman A, Berthelsen J G, Muller J, Von der Maase H, Skakkebaek N E. Screening for carcinoma in situ of the testis.  Int J Androl . 1987;  10 173-180
    PubMedGoogle Scholar
  • 24 Devroey P. Microsurgical epididymal sperm aspiration (MESA) and testicular sperm extraction (TESE): indications and results. ESHRE Campus Symposium, Tel Aviv, Israel, March 27-28, 1996
    Google Scholar
  • 25 Novero V, Goossens A, Tounaye H, Silber S J, Van Steirteghem C A, Devroey P. Seminoma discovered in two males undergoing successful testicular sperm extraction for intracytoplasmic sperm injection.  Fertil Steril . 1996;  65 1051-1054
    PubMedGoogle Scholar
  • 26 Verheyen G, Nagy Z, Joris H, De Croo I, Tournaye H, Van Steirteghem C A. Quality of frozen-thawed testicular sperm and first preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal-vesicle stage oocytes.  Fertil Steril . 1997;  67 74-80
    CrossrefPubMedGoogle Scholar
  • 27 Nogueira D, Bourgain C, Verheyen G, Van Steirteghem C A. Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies.  Hum Reprod . 1999;  14 2041-2049
    CrossrefPubMedGoogle Scholar
  • 28 Steele E, McClure N, Lewis S EM. Comparison of the effects of two methods of cryopreservation on testicular sperm DNA.  Fertil Steril . 2000;  74 450-453
    CrossrefPubMedGoogle Scholar
  • 29 Fishel S, Green S, Bishop M. Pregnancy after intracytoplasmatic injection of spermatid.  Lancet . 1995;  245 1641-1642
    PubMedGoogle Scholar
  • 30 Tesarik J. Sex chromosome abnormalities after intracytoplasmic sperm injection [letter].  Lancet . 1995;  346 1096
    PubMedGoogle Scholar
  • 31 Ogura A, Yanagimachi R. Round spermatid nuclei injected into hamster oocytes from pronuclei and participate in syngamy.  Biol Reprod . 1993;  48 219-225
    PubMedGoogle Scholar
  • 32 Ogura A, Matsuda J, Yanagimachi R. Birth of normal young after electrofusion of mouse oocytes with round spermatids.  Proc Nat Acad Sci U S A . 1994;  91 7460-7462
    PubMedGoogle Scholar
  • 33 Sofikitis N, Toda T, Miyagawa I. Reproductive capacity of the nucleus of the male gamete after completion of meiosis.  J Assist Reprod Genet . 1994;  11 335-341
    PubMedGoogle Scholar
  • 34 Kimura Y, Yanagimachi R. Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring.  Development . 1995;  121 2397-2405
    PubMedGoogle Scholar
  • 35 Edwards R G, Tarin J J, Dean N. Are spermatid injections into human oocytes now mandatory?.  Hum Reprod . 1994;  9 2217-2219
    PubMedGoogle Scholar
  • 36 Antinori S, Versaci C, Dani G. Successful fertilization and pregnancy after injection of frozen-thawed round spermatids into human oocytes.  Hum Reprod . 1997;  12 554-556
    CrossrefPubMedGoogle Scholar
  • 37 Sofikitis N V, Yamamoto Y, Miyagawa I. Ooplasmic injection of elongating spermatids for the treatment of non-obstructive azoospermia.  Hum Reprod . 1998;  13 709-714
    CrossrefPubMedGoogle Scholar
  • 38 Holstein A F, Rosen-Runge E C. Atlas of Human Spermatogenesis.  Berlin: Grosse Verlag 1981: 92-95
    Google Scholar
  • 39 Tesarik J, Mendoza C. Spermatid injection into human oocytes: I. Laboratory techniques and special features of zygote development.  Hum Reprod . 1996;  11 772-779
    PubMedGoogle Scholar
  • 40 Angelopoulos T, Krey L, McCullough A. A simple and objective approach to identifying human round spermatids.  Hum Reprod . 1997;  12 2208-2216
    CrossrefPubMedGoogle Scholar
  • 41 Sousa M, Mendoza C, Barros A, Tesarik J. Calcium responses of human oocytes after intracytoplasmic injection of leucocytes, spermatocytes and round spermatids.  Mol Hum Reprod . 1996;  11 853-857
    PubMedGoogle Scholar
  • 42 Mendoza C, Benkhalifa M, Cohen Bacrie P. Combined use of proacrosin immunocytochemistry and autosomal DNA in situ hybridisation for evaluation of human ejaculated germ cells.  Zygote . 1996;  4 279-283
    PubMedGoogle Scholar
  • 43 Mendoza C, Tesarik J. The occurrence and identification of round spermatids in the ejaculate of men with nonobstructive azoospermia.  Fertil Steril . 1996;  66 826-829
    PubMedGoogle Scholar
  • 44 Fishel S, Green S, Hunter A. Human fertilization with round and elongated spermatids.  Hum Reprod . 1997;  12 336-340
    CrossrefPubMedGoogle Scholar
  • 45 Silber S J, Johnson L. Are spermatid injections of any clinical value?.  <~>ROSNI and ROSI revisited: round spermatid nucleus injection and round spermatid injection. Hum Reprod . 1998;  13 509-515
    PubMedGoogle Scholar
  • 46 Tesarik J, Sousa M, Greco E, Mendoza C. Spermatids as gametes: indications and limitations.  Hum Reprod . 1998;  13 (suppl 3) 89-111
    PubMedGoogle Scholar
  • 47 Amer M, Soliman E, El-Sadek M. Is complete spermiogenesis failure a good indication for spermatid conception?.  Lancet . 1997;  350 116
    PubMedGoogle Scholar
  • 48 Tesarik J, Sousa M. More than 90% fertilization rates after intracytoplasmatic sperm injection and artificial induction of oocyte activation with calcium ionophore.  Fertil Steril . 1995;  63 343-349
    PubMedGoogle Scholar
  • 49 Vanderzwalmen P, Lejeune B, Nijs M. Fertilization of an oocyte microinseminated with a spermatid in an in-vitro fertilization program.  Hum Reprod . 1995;  10 502-503
    PubMedGoogle Scholar
  • 50 Tesarik J, Mendoza C. Genomic imprinting abnormalities: a new potential risk of assisted reproduction.  Mol Hum Reprod . 1996;  2 295-258
    PubMedGoogle Scholar
  • 51 Tesarik J, Greco E, Mendoza C. ROSI, instructions for use: 1997 update. Round spermatid injection.  Hum Reprod . 1998;  13 519-523
    PubMedGoogle Scholar
  • 52 Vanderzwalmen P, Nijs M, Schoysman R. The problems of spermatid microinjection in the human: the need for an accurate morphological approach and selective methods for viable and normal cells.  Hum Reprod . 1998;  13 515-519
    PubMedGoogle Scholar
  • 53 Fishel S, Aslam I, Tesarik J. Spermatid conception: a stage too early, or a time too soon?.  [editorial]. Hum Reprod . 1996;  11 1371-1375
    PubMedGoogle Scholar
  • 54 Aslam I, Fishel S. Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception.  Hum Reprod . 1998;  13 634-638
    CrossrefPubMedGoogle Scholar
  • 55 Kimura Y, Yanagimachi R. Development of normal mice from oocytes injected with secondary spermatocyte nuclei.  Biol Reprod . 1995;  53 855-862
    PubMedGoogle Scholar
  • 56 Bonduelle M, Aytoz A, Wilikens A. Prospective follow-up study of 1,987 children born after intracytoplasmic sperm injection (ICSI). In: Filicori M, Flamigni C, eds. Treatment of Infertility: The New Frontiers Princeton Junction, NJ: Communication Media for Education 1998: 445-461
    Google Scholar
  • 57 Glenn C, Driscoll D, Yang T, Nicholls R. Genomic imprinting: potential function and mechanisms revealed by the Prader-Willi and Angelman Syndromes.  Mol Hum Reprod . 1997;  3 321-332
    CrossrefPubMedGoogle Scholar
  • 58 Balet R, Egbase P, Grudzinskas J G. TESE and the distribution of spermatogenesis in the testicles of azoospermic men [letter].  Hum Reprod . 1998;  13 2034
    PubMedGoogle Scholar
  • 59 Silber S J. TESE and the distribution of spermatogenesis in the testicles of azoospermic men.  Hum Reprod . 1998;  13 2035
    PubMedGoogle Scholar
  • 60 Tesarik J, Mendoza C, Testart J. Viable embryos from injection of round spermatids into oocytes.  N Engl J Med . 1995;  333 525
    CrossrefPubMedGoogle Scholar
  • 61 Tesarik J. Fertilization of oocytes by injecting spermatozoa, spermatids and spermatocytes.  Rev Reprod . 1996;  1 149-152
    PubMedGoogle Scholar
  • 62 Antinori S, Versaci C, Dani G. Fertilization with human testicular spermatids: four successful pregnancies.  Hum Reprod . 1997;  12 286-291
    CrossrefPubMedGoogle Scholar
  • 63 Barak Y, Kogosowski A, Goldman S. Pregnancy and birth after transfer of embryos that developed from single-nucleated zygotes obtained by injection of round spermatids into oocytes.  Fertil Steril . 1998;  70 67-70
    CrossrefPubMedGoogle Scholar
  • 64 Barros A, Bernabeu R, Takahashi K. Intracytoplasmic injection of ejaculate and testicle spermatids: report on 35 cycles.  Hum Reprod . 1998;  13 (suppl1) 154-155
    CrossrefPubMedGoogle Scholar
  • 65 Bernabeu R, Cremades N, Takahashi K, Sousa M. Successful pregnancy after spermatid injection.  Hum Reprod . 1998;  13 1898-1900
    CrossrefPubMedGoogle Scholar
  • 66 Kahraman S, Polat G, Samli M. Multiple pregnancies obtained by testicular spermatid injection in combination with intracytoplasmic sperm injection.  Hum Reprod . 1998;  13 104-110
    CrossrefPubMedGoogle Scholar
  • 67 Mansour R T, Aboulghar M A, Serour G I. Pregnancy and delivery after intracytoplasmatic injection of spermatids into human oocytes.  Middle East Fertil Soc J . 1996;  1 223-225
    PubMedGoogle Scholar
  • 68 Araki Y, Motoyama M, Yoshida A. Intracytoplasmic injection with late spermatids: a successful procedure in achieving childbirth for couples in which the male partner suffers from azoospermia due to deficient spermatogenesis.  Fertil Steril . 1997;  67 559-561
    CrossrefPubMedGoogle Scholar
  • 69 Ghazzawi I, Al-Hasani S, Taher M, Souso S. Reproductive capacity of round spermatids compared with mature spermatozoa in a population of azoospermic men.  Hum Reprod . 1999;  14 736-740
    CrossrefPubMedGoogle Scholar
  • 70 Al-Hasani S, Ludwig M, Palermo I. Intracytoplasmic injection of round and elongated Spermatids from azoospermic patients: results and review.  Hum Reprod . 1994;  14(suppl) 97-107
    PubMedGoogle Scholar
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