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Catabolism through the threonine dehydrogenase pathway does not account for the high first-pass extraction rate of dietary threonine by the portal drained viscera in pigs

Published online by Cambridge University Press:  08 March 2007

Nathalie Le Floc'h  and
Bernard Sève
Nathalie Le Floc'h*
Affiliation:
Joint Research Unit for Calf and Pig Production, INRA 35590, Saint Gilles, France
Bernard Sève
Affiliation:
Joint Research Unit for Calf and Pig Production, INRA 35590, Saint Gilles, France
*
*Corresponding author: Dr Nathalie Le Floc'h, fax +33 2 23 48 50 80, email Nathalie.Lefloch@rennes.inra.fr
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Abstract

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In pigs the extensive threonine utilization by the splanchnic tissues explains the relative inefficiency of dietary threonine conversion for body protein accretion. Two experiments were conducted to estimate the contribution of the portal drained viscera (PDV) and the liver to threonine metabolism and especially catabolism in growing pigs. In the first experiment, four pigs were surgically prepared for chronic catheter insertion in the portal, hepatic and jugular veins and in the carotid artery. They were continuously infused with l-[1-13C]threonine through the jugular catheter. The PDV and total splanchnic viscera (PDV and the liver) extracted 14·3 and 18·8 % of arterial threonine input, respectively. In a second experiment, we studied the metabolism of dietary threonine in the PDV and the liver in six female growing pigs. Animals were surgically prepared as in the first experiment except that l-[1-13C]threonine and [15N]glycine were continuously infused in the duodenum for 10 h. Unlabelled and labelled threonine and glycine PDV, liver and splanchnic tissues balance were calculated from plasma samples taken during the last 2 h of this infusion. Splanchnic tissues extracted 60 % of infused labelled threonine, 88 % of which was extracted by PDV so that threonine extraction by the liver was low. Both the liver and the pancreas can degrade threonine through the l-threonine 3-dehydrogenase pathway but not the intestine. Our data suggest that threonine catabolism through the l-threonine 3-dehydrogenase pathway was only a minor component of total threonine utilization in the splanchnic tissues.

Keywords

PigsThreoninePortal drained visceraLiver
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 4 , April 2005 , pp. 447 - 456
Copyright
Copyright © The Nutrition Society 2005

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