nach oben

01.09.2016 | Laryngology | Ausgabe 2/2017

β-Defensin-2 is overexpressed in human vocal cord polyps

Zeitschrift:: European Archives of Oto-Rhino-Laryngology > Ausgabe 2/2017

Autoren:: Jinjin Gu, Yongwang Huang

Abstract

The objective of the study is to investigate the expression of human β-defensin-1 (hBD-1) and human β-defensin-2 (hBD-2) in vocal cord polyps using tissue microarray. Tissue specimens from vocal cord polyps (N = 51), vocal cord nodules (N = 26), and healthy vocal cords (N = 8) were retrieved from the biobank of the Department of Pathology of Tianjin Tianhe Hospital between 2003 and 2006 and immunostained on tissue microarrays for the quantitative analysis of hBD-1 and hBD-2 expression. hBD-1 expression did not differ significantly between healthy vocal cords, vocal cord nodules, and vocal cord polyps (p = 0.904). In contrast, hBD-2 expression was significantly higher in vocal cord polyps compared to vocal cord nodules and healthy vocal cords (p < 0.001). The expression of hBD-2, but not hBD-1, is elevated in vocal cord polyp epithelium. This suggests that hBD-1 has a more constitutive role in host defense in the vocal cords, whereas hBD-2 expression may be a result of local inflammation or the presence of invading pathogens.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Jetzt einloggen Kostenlos registrieren

★ PREMIUM-INHALT

e.Med Interdisziplinär

Für Ihren Erfolg in Klinik und Praxis - Die beste Hilfe in Ihrem Arbeitsalltag als Mediziner

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf SpringerMedizin.de.

Jetzt gratis testen * Jetzt kaufen

Sie können e.Med Interdisziplinär 14 Tage kostenlos testen (keine Print-Zeitschrift enthalten). Der Test läuft automatisch und formlos aus. Es kann nur einmal getestet werden.

e.Med HNO

Kombi-Abonnement

Mit e.Med HNO erhalten Sie Zugang zu CME-Fortbildungen des Fachgebietes HNO, den Premium-Inhalten der HNO-Fachzeitschriften, inklusive einer gedruckten HNO-Zeitschrift Ihrer Wahl.

Jetzt testen ¹

Johns MM (2003) Update on the etiology, diagnosis, and treatment of vocal fold nodules, polyps, and cysts. Curr Opin Otolaryngol Head Neck Surg. 11(6):456–461 CrossRefPubMed

Martins RHG, Defaveri J, Domingues MAC, de Albuquerque e Silva R (2010) Vocal polyps: clinical, morphological, and immunohistochemical aspects. J Voice. 25(1):98–106 CrossRefPubMed

Ganz T (2002) Epithelia: not just physical barriers. Proc Natl Acad Sci USA. 99(6):3357–3358 CrossRefPubMedPubMedCentral

Zhao Le, Lu W (2014) Defensins in innate immunity. Curr Opin Hematol 21(1):37–42 CrossRefPubMed

Hans M, Madaan Hans V (2014) Epithelial antimicrobial peptides: guardian of the oral cavity. Int J Pept 2014(1):370297 PubMedPubMedCentral

Semple F, Dorin JR (2012) β-Defensins: multifunctional modulators of infection, inflammation and more? J Innate Immun. 4(4):337–348 CrossRefPubMed

Pazgier M, Hoover DM, Yang D, Lu W, Lubkowski J (2006) Human beta-defensins. Cell Mol Life Sci 63(11):1294–1313 CrossRefPubMed

Chen P-H, Fang S-Y (2004) Expression of human beta-defensin 2 in human nasal mucosa. Eur Arch Otorhinolaryngol 261(5):238–241 CrossRefPubMed

Lee SH, Kim JE, Lim HH, Lee HM, Choi JO (2002) Antimicrobial defensin peptides of the human nasal mucosa. Ann Otol Rhinol Laryngol. 111(2):135–141 CrossRefPubMed

10.

Abiko Y, Saitoh M, Nishimura M, Yamazaki M, Sawamura D, Kaku T (2007) Role of beta-defensins in oral epithelial health and disease. Med Mol Morphol. 40(4):179–184 CrossRefPubMed

11.

McCray PB, Bentley L (1997) Human airway epithelia express a beta-defensin. Am J Respir Cell Mol Biol 16(3):343–349 CrossRefPubMed

12.

Marshall A, Hodgson J (1998) DNA chips: an array of possibilities. Nat Biotechnol 16(1):27–31 CrossRefPubMed

13.

Kononen J, Bubendorf L, Kallioniemi A, Bärlund M, Schraml P, Leighton S et al (1998) Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 4(7):844–847 CrossRefPubMed

14.

Rimm DL, Camp RL, Charette LA, Olsen DA, Provost E (2001) Amplification of tissue by construction of tissue microarrays. Exp Mol Pathol 70(3):255–264 CrossRefPubMed

15.

Nunes RB, Behlau M, Nunes MB, Paulino JG (2013) Clinical diagnosis and histological analysis of vocal nodules and polyps. Braz J Otorhinolaryngol. 79(4):434–440 CrossRefPubMed

16.

Cui C, Cheng J, Lin Z, Yi Z, Yang J (2002) Study on tyrosine phosphate gene expression in nasopharyngeal carcinoma. Chin J Otorhinolaryngol. 37(4):315–316

17.

Kong W (2002) Otolaryngology, 2nd edn. People’s Health Publishing House, Beijing

18.

Kambic V, Radsel Z, Zargi M, Acko M (1981) Vocal cord polyps: incidence, histology and pathogenesis. J Laryngol Otol 95(6):609–618 CrossRefPubMed

19.

Gray SD, Hammond E, Hanson DF (1995) Benign pathologic responses of the larynx. Ann Otol Rhinol Laryngol. 104(1):13–18 CrossRefPubMed

20.

Remacle M, Degols JC, Delos M (1996) Exudative lesions of Reinke’s space. An anatomopathological correlation. Acta Otorhinolaryngol Belg. 50(4):253–264 PubMed

21.

Wallis L, Jackson-Menaldi C, Holland W, Giraldo A (2004) Vocal fold nodule vs. vocal fold polyp: answer from surgical pathologist and voice pathologist point of view. J Voice 18(1):125–129 CrossRefPubMed

22.

Krisanaprakornkit S, Weinberg A, Perez CN, Dale BA (1998) Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect Immun 66(9):4222–4228 PubMedPubMedCentral

23.

O’Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T et al (1999) Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 163(12):6718–6724 PubMed

24.

Harder J, Bartels J, Christophers E, Schroder JM (1997) A peptide antibiotic from human skin. Nature 387(6636):861 CrossRefPubMed

25.

Ali RS, Falconer A, Ikram M, Bissett CE, Cerio R, Quinn AG (2001) Expression of the peptide antibiotics human beta defensin-1 and human beta defensin-2 in normal human skin. J Invest Dermatol. 117(1):106–111 CrossRefPubMed

26.

Liu L, Wang L, Jia HP, Zhao C, Heng HH, Schutte BC et al (1998) Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation. Gene 222(2):237–244 CrossRefPubMed

27.

Tsutsumi-Ishii Y, Nagaoka I (2002) NF-kappa B-mediated transcriptional regulation of human beta-defensin-2 gene following lipopolysaccharide stimulation. J Leukoc Biol 71(1):154–162 PubMed

28.

Harder J, Meyer-Hoffert U, Teran LM, Schwichtenberg L, Bartels J, Maune S et al (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol 22(6):714–721 CrossRefPubMed

29.

MacRedmond R, Greene C, Taggart CC, McElvaney N, O’Neill S (2005) Respiratory epithelial cells require toll-like receptor 4 for induction of human beta-defensin 2 by lipopolysaccharide. Respir Res 6:116 CrossRefPubMedPubMedCentral

30.

Tsutsumi-Ishii Y, Nagaoka I (2003) Modulation of human beta-defensin-2 transcription in pulmonary epithelial cells by lipopolysaccharide-stimulated mononuclear phagocytes via proinflammatory cytokine production. J Immunol. 170(8):4226–4236 CrossRefPubMed

31.

Duits LA, Ravensbergen B, Rademaker M, Hiemstra PS, Nibbering PH (2002) Expression of beta-defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells. Annu Rev Immunol 106(4):517–525

32.

O’Neil DA, Cole SP, Martin-Porter E, Housley MP, Liu L, Ganz T et al (2000) Regulation of human beta-defensins by gastric epithelial cells in response to infection with Helicobacter pylori or stimulation with interleukin-1. Infect Immun 68(9):5412–5415 CrossRefPubMedPubMedCentral

33.

Tsutsumi-Ishii Y, Nagaoka I (2002) NF-kappa B-mediated transcriptional regulation of human beta-defensin-2 gene following lipopolysaccharide stimulation. J Leukoc Biol 71(1):154–162 PubMed

Titel: β-Defensin-2 is overexpressed in human vocal cord polyps
Autoren:: Jinjin Gu
Yongwang Huang
Publikationsdatum: 01.09.2016
DOI: https://doi.org/10.1007/s00405-016-4270-4
Verlag: Springer Berlin Heidelberg
Zeitschrift: European Archives of Oto-Rhino-Laryngology and Head & Neck
Ausgabe 2/2017
Print ISSN: 0937-4477
Elektronische ISSN: 1434-4726

Sie können e.Med HNO 14 Tage kostenlos testen (keine Print-Zeitschrift enthalten). Der Test läuft automatisch und formlos aus. Es kann nur einmal getestet werden.

Neu im Fachgebiet HNO

Vergleich

Newsletter bestellen

Bildnachweise

Mail Icon II

Springer Medizin

β-Defensin-2 is overexpressed in human vocal cord polyps

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

e.Med HNO

Neu im Fachgebiet HNO

Die COVID-19-Impfquoten – in Deutschland und der Welt

Schützt Kiffen vor Oropharyngealtumoren?

Tipps zur Sterilisation von N95-Masken

Ärzte fühlen sich auf Corona-Impfgespräche gut vorbereitet

Newsletter

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

e.Med HNO

Weitere Artikel der Ausgabe 2/2017

Work-up of globus: assessing the benefits of neck ultrasound and videofluorography

Transoral robotic surgery for the management of obstructive sleep apnea: a systematic review and meta-analysis

Functional outcome after one-stage flap reconstruction of the hypopharynx following tumor ablation

Cross-cultural adaptation and validation of the SNOT-22 into Italian

Prophylactic exercises among head and neck cancer patients during and after swallowing sparing intensity modulated radiation: adherence and exercise performance levels of a 12-week guided home-based program

A new surgical technique versus an old marker: can expansion sphincter pharyngoplasty reduce C-reactive protein levels in patients with obstructive sleep apnea?

Neu im Fachgebiet HNO

Die COVID-19-Impfquoten – in Deutschland und der Welt

Schützt Kiffen vor Oropharyngealtumoren?

Tipps zur Sterilisation von N95-Masken

Ärzte fühlen sich auf Corona-Impfgespräche gut vorbereitet

Newsletter