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medizinische genetik

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01.12.2008 | Schwerpunkt

3D-Fluoreszenz-in-situ-Hybridisierung und Zellkernarchitektur

verfasst von: Dr. M. Cremer, S. Müller, I. Solovei, T. Cremer

Erschienen in: medizinische genetik | Ausgabe 4/2008

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Zusammenfassung

Fluoreszenz-in-situ-Hybridisierung an dreidimensional konservierten Zellkernen (3D-FISH) ist eine effiziente Methode für Untersuchungen zur 3D-Anordnung von Chromatin im Zellkern. Die Zellkernarchitektur stellt eine Ebene epigenetischer Mechanismen der Genregulation dar. 3D-FISH-Untersuchungen belegten eine große Variabilität in den Nachbarschaftsbeziehungen individueller Chromosomenterritorien im Zellkern. Im Gegensatz hierzu konnte eine distinkte radiale, von der Gendichte abhängige Anordnung von Chromatin gezeigt werden, die evolutionär hochkonserviert ist. Genreiches Material ist bevorzugt in der Kernmitte, genarmes in der Kernperipherie angeordnet. Die Frage einer räumlichen Assoziation kotranskriptionell exprimierter Gene (so genannte „expression hubs”) wird derzeit kontrovers diskutiert.

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Titel: 3D-Fluoreszenz-in-situ-Hybridisierung und Zellkernarchitektur
verfasst von: Dr. M. Cremer
S. Müller
I. Solovei
T. Cremer
Publikationsdatum: 01.12.2008
Verlag: Springer-Verlag
Erschienen in: medizinische genetik / Ausgabe 4/2008
Print ISSN: 0936-5931
Elektronische ISSN: 1863-5490
DOI: https://doi.org/10.1007/s11825-008-0132-9

Springer Medizin

Zusammenfassung

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