nach oben

Forensic Science, Medicine and Pathology

Erschienen in:

27.05.2023 | Original Article

A beetle for prosecution: exogenous DNA detection from larval and adult gut of a Neotropical carrion beetle

verfasst von: Alessandra Santana Batista Toni, José Eduardo Serrão, Verônica Saraiva Fialho

Erschienen in: Forensic Science, Medicine and Pathology | Ausgabe 2/2023

Einloggen, um Zugang zu erhalten

Abstract

The use of recovered DNA ingested by necrophagous or hematophagous insects has increased in forensic sciences, mainly with representatives of flies. However, some beetles are also important for medico-legal forensic entomology because they feed on carcasses until advanced decomposition. This study evaluated whether the Neotropical carrion beetle Oxelytrum discicolle (Silphidae) has the potential for the detection of exogenous DNA into the gut. The whole gut or the gut contents were extracted from O. discicolle larvae and adult previously fed on pig carcass. The pig DNA recovery rate was 33.3% in larvae and 25% in adults, indicating that the carrion beetle’s gut may be useful for DNA identification of ingested food. Samples with the whole gut or only gut contents showed the same DNA recovery rate. Exogenous DNA from the whole gut was recovered from samples stored in ethanol at -20 ºC for 11 days, showing that samples of O. discicolle can be stored in the forensic laboratory without loss in DNA recovery rate.

Nur mit Berechtigung zugänglich

Mohammad Z, Alajmi R, Alkuriji M, Metwally D, Kaakeh W, Almeaiweed N. Role of Chrysomya albiceps (Diptera: Calliphoridae) and Musca domestica (Diptera: Muscidae) maggot crop contents in identifying unknown cadavers. J Med Entomol. 2020;58:93–8.

Njau DG, Muge EK, Kinyanjui PW, Omwandho COA, Mukwana S. STR analysis of human DNA from maggots fed on decomposing bodies: Assessment of the time period for successful analysis. Egypt J Forensic Sci. 2016;6:261–9.CrossRef

Chávez-Briones M de L, Hernández-Cortés R, Díaz-Torres P, Niderhauser-García A, Ancer-Rodríguez J, Jaramillo-Rangel G, et al. Identification of human remains by DNA analysis of the gastrointestinal contents of fly larvae. J Forensic Sci. 2013;58:248–50.

Vinueza-Espinosa DC, Santos C, Martínez-Labarga C, Malgosa A. Human DNA extraction from highly degraded skeletal remains: How to find a suitable method ? Electrophoresis. 2020;41:2149–58.CrossRef

Catts EP, Goff ML. Forensic entomology in criminal investigations. Annu Rev Entomol. 1992;37:253–72.CrossRefPubMed

Durdle A. Insects as vectors of DNA in a forensic context. WIREs Forensic Sci. 2020;2:1–27.CrossRef

Campobasso CP, Linville JG, Wells JD, Introna F. Forensic genetic analysis of insect gut contents. Am J Forensic Med Pathol. 2005;26:161–5.CrossRefPubMed

Wells JD, Introna F, Di Vella G, Campobasso CP, Hayes J, Sperling FAH. Human and Insect Mitochondrial DNA Analysis from Maggots. J Forensic Sci. 2001;46:15022J.CrossRef

Mukherjee S, Singh P, Tuccia F, Pradelli J, Giordani G, Vanin S. DNA characterization from gut content of larvae of Megaselia scalaris (Diptera, Phoridae). Sci Justice [Internet]. Elsevier; 2019;59:654–9. Available from: https://www.sciencedirect.com/science/article/abs/pii/S1355030619300656?via%3Dihub.

10.

Chamoun CA, Couri MS, Louro ID, Garrido RG, Moura-Neto RS, Oliveira-Costa J. In vitro recovery and identification of Y-STR DNA from Chrysomya albiceps (Diptera, Calliphoridae) larvae fed a decomposing mixture of human semen and ground beef. Genet Mol Res. 2019;18:1–11.CrossRef

11.

Chamoun CA, Couri MS, Garrido RG, Moura-Neto RS, Oliveira-Costa J. Recovery & identification of human Y-STR DNA from immatures of chrysomya albiceps (Diptera: Calliphoridae). Simulation of sexual crime investigation involving victim corpse in state of decay. Forensic Sci Int. 2020;310:110239.

12.

Marchetti D, Arena E, Boschi I, Vanin S. Human DNA extraction from empty puparia. Forensic Sci Int Elsevier Ireland Ltd. 2013;229:e26-9.CrossRef

13.

Durdle A, Mitchell RJ, van Oorschot RAH. The human DNA content in artifacts deposited by the blowfly Lucilia cuprina fed human blood, semen and saliva. Forensic Sci Int Elsevier Ireland Ltd. 2013;233:212–9.CrossRef

14.

Durdle A, van Oorschot RAH, Mitchell RJ. The transfer of human DNA by Lucilia cuprina (Meigen) (Diptera: Calliphoridae). Forensic Sci Int Genet Suppl Ser. 2009;2:180–2.

15.

Curic G, Hercog R, Vrselja Z, Wagner J. Identification of person and quantification of human DNA recovered from mosquitoes (Culicidae). Forensic Sci Int Genet. 2014;8:109–12.CrossRefPubMed

16.

Ansell J, Hu JT, Gilbert SC, Hamilton KA, Hill AVS, Lindsay SW. Improved method for distinguishing the human source of mosquito blood meals between close family members. Trans R Soc Trop Med Hyg. 2000;94:572–4.CrossRefPubMed

17.

Vieira BRC, Carvalho EF, Silva DA. Analysis of human DNA present in the digestive tract of Aedes aegypti mosquitoes for possible forensic application. Forensic Sci Int Genet Suppl Ser. Elsevier; 2017;6:e324–6.

18.

Kreike J, Kampfer S. Isolation and characterization of human DNA from mosquitoes (Culicidae). Int J Legal Med. 1999;112:380–2.CrossRefPubMed

19.

Chow-Shaffer E, Sina B, Hawley WA, De Benedictis J, Scott TW. Laboratory and field evaluation of polymerase chain reaction-based forensic DNA profiling for use in identification of human blood meal sources of Aedes aegypti (Diptera: Culicidae). J Med Entomol. 2000;37:492–502.CrossRefPubMed

20.

Davey JS, Casey CS, Burgess IF, Cable J. DNA detection rates of host mtDNA in bloodmeals of human body lice (Pediculus humanus L., 1758). Med Vet Entomol. 2007;21:293–6.

21.

Lord WD, DiZinno JA, Wilson MR, Budowle B, Taplin D, Meinking TL. Isolation, amplification, and sequencing of human mitochondrial DNA obtained from human crab louse, Pthirus Pubis (L.), blood meals. J Forensic Sci. 1998;43:14367J.

22.

Mumcuoglu KY, Gallili N, Reshef A, Brauner P, Grant H. Use of human lice in forensic entomology. J Med Entomol. 2009;41:803–6.CrossRef

23.

Schal C, Czado N, Gamble R, Barrett A, Weathers K, Lodhi KM. Isolation, identification, and time course of human DNA typing from bed bugs, Cimex lectularius. Forensic Sci Int Elsevier Ireland Ltd. 2018;293:1–6.CrossRef

24.

Midgley JM, Villet MH. Development of Thanatophilus micans (Fabricius 1794) (Coleoptera: Silphidae) at constant temperatures. Int J Legal Med [Internet]. 2009;123:285–92. Available from: https://doi.org/10.1007/s00414-008-0280-0.

25.

Midgley JM, Richards CS, Villet MH. The utility of Coleoptera in forensic investigations. In: Amendt J, Goff ML, Campobasso CP, Grassberger M, editors. Curr Concepts Forensic Entomol [Internet]. Dordrecht: Springer Netherlands; 2009. p. 57–68. Available from: https://doi.org/10.1007/978-1-4020-9684-6_4.

26.

DiZinno JA, Lord WD, Collins-Morton MB, Wilson MR, Goff ML. Mitochondrial DNA sequencing of beetle larvae (Nitidulidae: Omosita) recovered from human bone. J Forensic Sci [Internet]. United States; 2002;47:1337–9. Available from: https://pubmed.ncbi.nlm.nih.gov/12455660/.

27.

Peck SB, Anderson RS. Taxonomy, phylogeny and biogeography of the carrion beetles of Latin America (Coleoptera: Silphidae). Quaest Entomol. 1985;21:247–317.

28.

Lira LA, Vasconcelos SD. New record and update on the distribution Oxelytrum discicolle (Brullé, 1840) (Coleoptera: Silphidae) in South America. Coleopt Bull. 2016;70:399–402.CrossRef

29.

Lira LA, Macedo MP, Pujol-Luz JR, Vasconcelos SD. Diel activity and effect of carcass decomposition on the attractiveness to the forensically important species Oxelytrum discicolle (Coleoptera: Silphidae). J Forensic Sci [Internet]. John Wiley & Sons, Ltd; 2019;64:799–804. Available from: https://doi.org/10.1111/1556-4029.13954.

30.

Velásquez Y, Viloria AL. Instar determination of the Neotropical beetle Oxelytrum discicolle (Coleoptera: Silphidae). J Med Entomol. 2010;47:723–6.CrossRefPubMed

31.

Keh B. Scope and applications of forensic entomology. Annu Rev Entomol. 1985;30:137–54.CrossRefPubMed

32.

Lira LA, Barros-Cordeiro KB, Figueiredo B, Galvão MF, Frizzas MR. The carrion beetle Oxelytrum discicolle (Coleoptera: Silphidae) and the estimative of the minimum post-mortem interval in a forensic case in Brasília, Brazil. Rev Bras Entomol. 2020;64.

33.

Nichols E, Spector S, Louzada J, Larsen T, Amequita S, Favila ME, et al. Ecological functions and ecosystem services provided by Scarabaeinae dung beetles. Biol Conserv. 2008;141:1461–74.CrossRef

34.

Barton PS, Evans MJ. Insect biodiversity meets ecosystem function: differential effects of habitat and insects on carrion decomposition. Ecol Entomol [Internet]. John Wiley & Sons, Ltd; 2017;42:364–74. Available from: https://doi.org/10.1111/een.12395.

35.

Ridgeway JA, Midgley JM, Collett IJ, Villet MH. Advantages of using development models of the carrion beetles Thanatophilus micans (Fabricius) and T. mutilatus (Castelneau) (Coleoptera: Silphidae) for estimating minimum post mortem intervals, verified with case data. Int J Legal Med [Internet]. 2014;128:207–20. Available from: https://doi.org/10.1007/s00414-013-0865-0.

36.

Gruszka J, Krystkowiak-Kowalska M, Frątczak-Łagiewska K, Mądra-Bielewicz A, Charabidze D, Matuszewski S. Patterns and mechanisms for larval aggregation in carrion beetle Necrodes littoralis (Coleoptera: Silphidae). Anim Behav [Internet]. 2020;162:1–10. Available from: https://www.sciencedirect.com/science/article/pii/S0003347220300269.

37.

Gruszka J, Matuszewski S. Temperature models of development for Necrodes littoralis L. (Coleoptera: Silphidae), a carrion beetle of forensic importance in the Palearctic region. Sci Rep [Internet]. 2022;12:9689. Available from: https://doi.org/10.1038/s41598-022-13901-y.

38.

Jakubec P, Novák M, Qubaiová J, Šuláková H, Růžička J. Description of immature stages of Thanatophilus sinuatus (Coleoptera: Silphidae). Int J Legal Med [Internet]. 2019;133:1549–65. Available from: https://doi.org/10.1007/s00414-019-02040-1.

39.

Novák M, Jakubec P, Qubaiová J, Šuláková H, Růžička J. Revisited larval morphology of Thanatophilus rugosus (Coleoptera: Silphidae). Int J Legal Med [Internet]. 2018;132:939–54. Available from: https://doi.org/10.1007/s00414-017-1764-6.

40.

Qubaiová J, Jakubec P, Montoya-Molina S, Novák M, Šuláková H. Influence of Diet on Development and Survival of Thanatophilus rugosus (Coleoptera: Silphidae). J Med Entomol [Internet]. 2021;58:2124–9. Available from: https://doi.org/10.1093/jme/tjab141.

41.

Qubaiová J, Jakubec P, Montoya-Molina S, Novák M, Šuláková H. Diet impact on the development and survival of Oiceoptoma thoracicum (Coleoptera: Silphidae). J Med Entomol [Internet]. 2022;59:1905–1910. Available from: https://doi.org/10.1093/jme/tjac129.

42.

Toni ASB, Fialho VS, Cossolin JFS, Serrão JE. Larval and adult digestive tract of the carrion beetle Oxelytrum discicolle (Brullé, 1840) (Coleoptera: Silphidae). Arthropod Struct Dev [Internet]. 2022;71. Available from: https://www.sciencedirect.com/science/article/pii/S1467803922000743.

43.

Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870–4.

44.

Dooley JJ, Paine KE, Garrett SD, Brown HM. Detection of meat species using TaqMan real-time PCR assays. Meat Sci. 2004;68:431–8.CrossRefPubMed

45.

Souza ER, Rafael JA, Filho FFX, Da-Silva-Freitas JO, Oliveira-Costa J, Ururahy-Rodrigues A. First medicolegal forensic entomology case of Central Amazon: a suicide by hanging with incomplete suspension. EntomoBrasilis. 2014;7:12–5.CrossRef

46.

Skowronek R, Tomsia M, Droździok K, Kabiesz J. Insects feeding on cadavers as an alternative source of human genetic material. Arch Med sa̧dowej i Kryminol. 2014;64:254–67.

47.

Caccia S, Casartelli M, Tettamanti G. The amazing complexity of insect midgut cells: types, peculiarities, and functions. Cell Tissue Res. Cell and Tissue Research; 2019;377:505–25.

48.

Holtof M, Lenaerts C, Cullen D, Vanden Broeck J. Extracellular nutrient digestion and absorption in the insect gut. Cell Tissue Res. Cell and Tissue Research; 2019;377:397–414.

49.

Billingsley PF, Lehane MJ. Structure and ultrastructure of the insect midgut. Biol Insect Midgut. 1996. p. 3–30.

50.

Billingsley PF. The midgut ultrastructure of hematophagous insects. Annu Rev Entomol [Internet]. Annual Reviews; 1990;35:219–48. Available from: https://doi.org/10.1146/annurev.en.35.010190.001251.

51.

Di Luise E, Magni P, Staiti N, Spitaleri S, Romano C. Genotyping of human nuclear DNA recovered from the gut of fly larvae. Forensic Sci Int Genet Suppl Ser. 2008;1:591–2.CrossRef

52.

Kondakci GO, Bulbul O, Shahzad MS, Polat E, Cakan H, Altuncul H, et al. STR and SNP analysis of human DNA from Lucilia sericata larvae’s gut contents. Forensic Sci Int Genet Suppl Ser. 2009;2:178–9.CrossRef

53.

Zehner R, Amendt J, Krettek R. STR typing of human DNA from fly larvae fed on decomposing bodies. J Forensic Sci. 2004;49:1–4.CrossRef

54.

Reeves LE, Holderman CJ, Gillett-Kaufman JL, Kawahara AY, Kaufman PE. Maintenance of host DNA integrity in field-preserved mosquito (Diptera: Culicidae) blood meals for identification by DNA barcoding. Parasites and Vectors Parasites & Vectors. 2016;9:1–11.

55.

Linville JG, Hayes J, Wells J. Mitochondrial DNA and STR analyses of maggot crop contents: effect of specimen preservation technique. J Forensic Sci. 2004;49:341–4.CrossRefPubMed

56.

Wilson MR, Stoneking M, Holland MM, DiZinno JA. Guidelines for the use of mitochondrial DNA sequencing in forensic science. Crime Lab Dig. 1993;20:68–77.

57.

Schoenly KG, Haskell NH, Mills DK, Bieme-Ndi C, Larsen K, Lee Y. Recreating death’s acre in the school yard: Using pig carcasses as model corpses to teach concepts of forensic entomology & ecological succession. Am Biol Teach [Internet]. 2006;68:402–10. Available from: https://doi.org/10.1662/0002-7685(2006)68[402:RDAITS]2.0.CO;2.

58.

Matuszewski S, Hall MJR, Moreau G, Schoenly KG, Tarone AM, Villet MH. Pigs vs people: The use of pigs as analogues for humans in forensic entomology and taphonomy research. Int J Legal Med [Internet]. 2020;134:793–810. Available from: https://doi.org/10.1007/s00414-019-02074-5.

Titel: A beetle for prosecution: exogenous DNA detection from larval and adult gut of a Neotropical carrion beetle
verfasst von: Alessandra Santana Batista Toni
José Eduardo Serrão
Verônica Saraiva Fialho
Publikationsdatum: 27.05.2023
Verlag: Springer US
Erschienen in: Forensic Science, Medicine and Pathology / Ausgabe 2/2023
Print ISSN: 1547-769X
Elektronische ISSN: 1556-2891
DOI: https://doi.org/10.1007/s12024-023-00658-7

Neu im Fachgebiet Pathologie

01.04.2024 | Forensische Traumatologie | CME

Springer Medizin

A beetle for prosecution: exogenous DNA detection from larval and adult gut of a Neotropical carrion beetle

Abstract

Neu im Fachgebiet Pathologie

Theoretische Grundlagen von Explosionen und Explosionsverletzungen

Auswirkungen vorgeschalteter Laborprozesse auf die Digitalisierung histologischer Schnittpräparate

Knochenmarkhistologie bei Zytopenien

Herausforderungen der Automation bei der quantitativen Auswertung von Leberbiopsien

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2023

A suicide attempt by intramuscular injection of pentobarbital sodium into rectus abdominis suggested by computed tomography

Correction to: HIV contact notification in sexual assault cases—ethical, legal, and procedural challenges

Lichtenberg figures—morphological findings

The biochemistry of the vitreous humour in estimating the post-mortem interval—a review of the literature, and use in forensic practice in Galicia (northwestern Spain)

A ruptured thoracic aortic aneurysm and the difficulties of confirming syphilis

Pregnancy-associated aortopathy and sudden postpartum death

Neu im Fachgebiet Pathologie

Theoretische Grundlagen von Explosionen und Explosionsverletzungen

Auswirkungen vorgeschalteter Laborprozesse auf die Digitalisierung histologischer Schnittpräparate

Knochenmarkhistologie bei Zytopenien

Herausforderungen der Automation bei der quantitativen Auswertung von Leberbiopsien