A comparison of the MeltPro^® HPV Test with the Cobas^® HPV Test for detecting and genotyping 14 high-risk human papillomavirus types

The clinical performance of the newly developed MeltPro^® HPV Test, based on multicolor melting curve analysis, was evaluated and compared with the commercially available Cobas^® HPV Test for detection of HPV and genotyping of HPV-16 and HPV-18. A total of 1647 cervical samples were analyzed with both tests. The agreement values were 96.2% for HPV detection, 99.6% for HPV-16 identification, and 99.7% for HPV-18 identification. All genotyping results from MeltPro^® HPV Test showed that HPV-52, HPV-58, and HPV-16 were the most common types in this study. Intra-laboratory reproducibility studies showed 97.8% agreement while inter-laboratory reproducibility studies showed 96.9% agreement for the MeltPro^® HPV Test. The MeltPro^® HPV Test and Cobas^® HPV Test are highly correlative and are useful for monitoring HPV infection.