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23.07.2018 | Original Article

A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.

verfasst von: Monique Beuve, Jean-Michel Hily, Antoine Alliaume, Catherine Reinbold, Jean Le Maguet, Thierry Candresse, Etienne Herrbach, Olivier Lemaire

Erschienen in: Archives of Virology | Ausgabe 11/2018

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Abstract

We have characterized the virome of a grapevine Pinot Noir accession (P70) that displayed, over the year, very stable and strong leafroll symptoms. For this, we have used two extraction methods (dsRNA and total RNA) coupled with the high throughput sequencing (HTS) Illumina technique. While a great disparity in viral sequences were observed, both approaches gave similar results, revealing a very complex infection status. Five virus and viroid isolates [Grapevine leafroll-associated viruse-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated virus (GRSPaV), Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1)] were detected in P70 with a grand total of eleven variants being identified and de novo assembled. A comparison between both extraction methods regarding their power to detect viruses and the ease of genome assembly is also provided.

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Titel: A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.
verfasst von: Monique Beuve
Jean-Michel Hily
Antoine Alliaume
Catherine Reinbold
Jean Le Maguet
Thierry Candresse
Etienne Herrbach
Olivier Lemaire
Publikationsdatum: 23.07.2018
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 11/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3949-9

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A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.

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