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22.06.2021 | Original Article

A Decreased Response to Resistin in Mononuclear Leukocytes Contributes to Oxidative Stress in Nonalcoholic Fatty Liver Disease

verfasst von: Cecilia C. Garcia, Bárbara Piotrkowski, Plácida Baz, Daniel Poncino, Javier Benavides, Luis Colombato, María Laura Reyes Toso, Silvina Yantorno, Valeria Descalzi, Gabriel E. Gondolesi, Cesar G. Fraga, Alejandra C. Cherñavsky

Erschienen in: Digestive Diseases and Sciences | Ausgabe 7/2022

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Abstract

Background

Deregulation of immune response and oxidative stress contribute to nonalcoholic fatty liver disease (NAFLD) pathogenesis. Resistin is a physiological modulator of inflammation and redox homeostasis of different cell types. Increased resistin serum concentration and the direct association between resistin hepatic expression and NAFLD severity suggest that resistin participates in NAFLD pathogenesis.

Aims

To evaluate resistin-induced regulation of redox homeostasis in mononuclear leukocytes from NAFLD patients and controls.

Methods

We evaluated basal and resistin-mediated modulation of reactive oxygen species (ROS) and glutathione content by flow cytometry, and antioxidant enzyme activities by spectrophotometry.

Results

Peripheral blood mononuclear cells (PBMC) from NAFLD patients showed higher ROS content and glutathione peroxidase activity and lower glutathione content, superoxide dismutase and glutathione reductase activities than control PBMC. Resistin decreased ROS levels and superoxide dismutase activity and increased glutathione reductase and catalase activities in PBMC from controls but not from patients. Resistin decreased glutathione content in PBMC from control and NAFLD patients, with greater effect on patient cells. Basal and resistin-modulated ROS levels were directly associated with obesity-related risk factors for NAFLD. Hepatic myeloid cells and T-lymphocytes from NAFLD patients showed higher basal ROS content than cells from controls. Resistin decreased ROS levels in hepatic T-lymphocytes from controls but not from patients.

Conclusions

Resistin regulates redox homeostasis in mononuclear leukocytes. A decreased response to resistin in leukocytes from NAFLD patients is associated with an impaired redox homeostasis.

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Titel: A Decreased Response to Resistin in Mononuclear Leukocytes Contributes to Oxidative Stress in Nonalcoholic Fatty Liver Disease
verfasst von: Cecilia C. Garcia
Bárbara Piotrkowski
Plácida Baz
Daniel Poncino
Javier Benavides
Luis Colombato
María Laura Reyes Toso
Silvina Yantorno
Valeria Descalzi
Gabriel E. Gondolesi
Cesar G. Fraga
Alejandra C. Cherñavsky
Publikationsdatum: 22.06.2021
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 7/2022
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-021-07105-z

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A Decreased Response to Resistin in Mononuclear Leukocytes Contributes to Oxidative Stress in Nonalcoholic Fatty Liver Disease

Abstract

Background

Aims

Methods

Results

Conclusions

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Abstract

Background

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Methods

Results

Conclusions

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