The online version of this article (doi:10.1186/1475-2875-11-107) contains supplementary material, which is available to authorized users.
Laurens Manning, Anna Rosanas-Urgell contributed equally to this work.
The authors declare that they have no competing interests.
LM co-ordinated the main study, obtained biopsies and produced the initial draft of the paper. AR-U performed the PCR assays and edited drafts of the paper. ML assisted clinically with the main study, obtained biopsies and edited drafts of the paper. HE assisted with data collection and specimen preparation, and edited drafts of the paper. CM performed the histological examination of brain tissue and edited drafts of the paper. IM was a co-investigator on the main study and edited drafts of the paper. PS was a co-investigator on the main study and edited drafts of the paper. TMED was the principal investigator of the main study, conceived the sub-study and produced the final version of the paper. All authors read and approved the final manuscript.
Microvascular sequestration of Plasmodium falciparum underlies cerebral malaria. Despite suggestive ex vivo evidence, this phenomenon has not been convincingly demonstrated in coma complicating Plasmodium vivax malaria. Severely-ill Papua New Guinean children with mixed P. falciparum/P. vivax infections are more likely to develop cerebral malaria and die than those with P. falciparum alone, possibly reflecting P. vivax sequestration. Nested PCR was performed on post mortem brain tissue from three such children dying from cerebral malaria due to mixed-species infections. No P. vivax DNA was detected. These findings do not support the hypothesis that P. vivax sequestration occurs in human brain.
Authors’ original file for figure 112936_2011_2091_MOESM1_ESM.tiff
Manning L, Laman M, Law I, Bona C, Aipit S, Teine D, Warrell J, Rosanas-Urgell A, Lin E, Kiniboro B, Vince J, Hwaiwhanje I, Karunajeewa H, Michon P, Siba P, Mueller I, Davis TM: Features and prognosis of severe malaria caused by Plasmodium falciparum, Plasmodium vivax and mixed Plasmodium species in Papua New Guinean children. PLoS One. 2011, 6: e29203-10.1371/journal.pone.0029203. PubMedCentralCrossRefPubMed
Kochar DK, Tanwar GS, Khatri PC, Kochar SK, Sengar GS, Gupta A, Kochar A, Middha S, Acharya J, Saxena V, Pakalapati D, Garg S, Das A: Clinical features of children hospitalized with malaria-a study from Bikaner, northwest India. Am J Trop Med Hyg. 2010, 83: 981-989. 10.4269/ajtmh.2010.09-0633. PubMedCentralCrossRefPubMed
Anstey NM, Handojo T, Pain MC, Kenangalem E, Tjitra E, Price RN, Maguire GP: Lung injury in vivax malaria: pathophysiological evidence for pulmonary vascular sequestration and posttreatment alveolar-capillary inflammation. J Infect Dis. 2007, 195: 589-596. 10.1086/510756. PubMedCentralCrossRefPubMed
Carvalho BO, Lopes SC, Nogueira PA, Orlandi PP, Bargieri DY, Blanco YC, Mamoni R, Leite JA, Rodrigues MM, Soares IS, Oliveira TR, Wunderlich G, Lacerda MV, del Portillo HA, Araújo MO, Russell B, Suwanarusk R, Snounou G, Rénia L, Costa FT: On the cytoadhesion of Plasmodium vivax- infected erythrocytes. J Infect Dis. 2010, 202: 638-647. 10.1086/654815. CrossRefPubMed
Chotivanich K, Udomsangpetch D, Pukrittayakamee A, Looareesuwan S, Beeson J, Day N, White N: The adhesion receptors of P. vivax-infected red cells. Exp Parasitol. 2003, 105: 33-34.
Snounou G, Viriyakosol S, Jarra W, Thaithong S, Brown KN: Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol. 1993, 58: 283-292. 10.1016/0166-6851(93)90050-8. CrossRefPubMed
Rosanas-Urgell A, Mueller D, Betuela I, Barnadas C, Iga J, Zimmerman PA, del Portillo HA, Siba P, Mueller I, Felger I: Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea. Malar J. 2010, 9: 361-10.1186/1475-2875-9-361. PubMedCentralCrossRefPubMed
Davis TM, Krishna S, Looareesuwan S, Supanaranond W, Pukrittayakamee S, Attatamsoonthorn K, White NJ: Erythrocyte sequestration and anemia in severe falciparum malaria. Analysis of acute changes in venous hematocrit using a simple mathematical model. J Clin Invest. 1990, 86: 793-800. 10.1172/JCI114776. PubMedCentralCrossRefPubMed
Pongponratn E, Turner GD, Day NP, Phu NH, Simpson JA, Stepniewska K, Mai NT, Viriyavejakul P, Looareesuwan S, Hien TT, Ferguson DJ, White NJ: An ultrastructural study of the brain in fatal Plasmodium falciparum malaria. Am J Trop Med Hyg. 2003, 69: 345-359. PubMed
- A histopathologic study of fatal paediatric cerebral malaria caused by mixed Plasmodium falciparum/Plasmodium vivax infections
Timothy ME Davis
- BioMed Central
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