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13.03.2020 | Brief Report

A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants

verfasst von: Marlonni Maurastoni, Tathiana F. Sá-Antunes, Scarlett A. Oliveira, Alexandre M. C. Santos, José A. Ventura, Patricia M. B. Fernandes

Erschienen in: Archives of Virology | Ausgabe 5/2020

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Abstract

Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.

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Titel: A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants
verfasst von: Marlonni Maurastoni
Tathiana F. Sá-Antunes
Scarlett A. Oliveira
Alexandre M. C. Santos
José A. Ventura
Patricia M. B. Fernandes
Publikationsdatum: 13.03.2020
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 5/2020
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04588-5

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A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants

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