Electronic supplementary material
The online version of this article (doi:10.1186/1471-2261-14-164) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
LJF and ZC designed the whole study, LJF, ZC, LJ, WLP, LYC performed the experiment, ZC,YHG wrote the paper, YWC collected and organized the data. All authors read and approved the final manuscript.
To explore the feasibility of a new method of achieving a permanent A-V block animal model.
16 beagles were randomly divided into two groups based on the method of their pre-implanted biventricular pacemakers. (1) In the first group (8 beagles), the A-V block model was achieved by ablating his-bundle potential at the site of the left ventricular superior-septum, under the aortic sinus, through femoral artery. (2) In the second group (8 beagles), the A-V block model was achieved by ablating his-bundle potential at the triangle of Koch, through femoral vein. A complete A-V block model was achieved as a standard in this study. The success rates, intraoperative arrhythmias, operative and X-ray exposure time, intraoperative bleeding amount were assessed in this two groups, both animal models were followed up for four weeks and then fasted to monitor myocardial pathological changes.
The success rate of the first group, which with fewer intraoperative arrhythmias, and less operative and X-ray exposure time, was significantly higher than the second group.
Compared with traditional animal method, our new method of ablating his-bundle potential at the left ventricle from the femoral artery has a higher success rate, fewer occurrence of malignant arrhythmias, and less operation and X-ray time. Thus, our new method should be preferred in the building of Permanent A-V Block Model.