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01.12.2017 | Research | Ausgabe 1/2017 Open Access

Malaria Journal 1/2017

A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity

Zeitschrift:
Malaria Journal > Ausgabe 1/2017
Autoren:
Setthakit Chitsanoor, Sangdao Somsri, Panyu Panburana, Mathirut Mungthin, Ratawan Ubalee, Maliwan Emyeam, Somchai Jongwutiwes, Jetsumon Sattabongkot, Rachanee Udomsangpetch
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12936-017-1781-4) contains supplementary material, which is available to authorized users.

Abstract

Background

To date, human peripheral blood mononuclear cells (PBMCs) have been used mainly in immune stimulation assays and the interpretation of data can be influenced by the previous immunological history of donors and cross reactivity with other infectious agents. Resolving these limitations requires an alternative in vitro model to uncover the primary response profiles.

Methods

A novel in vitro model of mononuclear cells (MNCs) generated from haematopoietic stem cells (HSCs) was developed and these cells were then co-cultured with various antigens from Plasmodium falciparum and Plasmodium vivax to investigate the response of naïve immune cells to malaria antigens by flow cytometry.

Results

In vitro stimulation of naïve lymphocytes showed that CD4+ and CD8+ T lymphocytes were significantly reduced (P < 0.01) by exposure to lysates of infected erythrocytes or intact erythrocytes infected with P. falciparum. The depletion was associated with the expression of CD95 (Fas receptor) on the surface of T lymphocytes. Maturation of T lymphocytes was affected differently, showing elevated CD3+CD4+CD8+ and CD3+CD4CD8 T lymphocytes after stimulation with cell lysates of P. falciparum and P. vivax, respectively. In addition, antigen presenting monocytes and dendritic cells derived from haematopoietic stem cells showed impaired HLA-DR expression as a consequence of exposure to different species of malaria parasites.

Conclusion

These results suggest that naïve mononuclear cells differentiated in vitro from HSCs could provide a valid model for the assessment of immunity. P. falciparum and P. vivax malaria parasites could modulate various populations of immune cells starting from newly differentiated mononuclear cells.
Zusatzmaterial
Additional file 1. Fluorescent dye-conjugated monoclonal antibodies used for characterizing of cell phenotypes.
Additional file 2. Responses of naïve T lymphocytes to malaria parasites.
Additional file 3. Responses of naïve T lymphocytes to malaria parasites (absolute number).
Additional file 4. Malaria parasites induced the expression of CD95 on T lymphocytes.
Additional file 5. Effects of malaria parasites on the antigen presenting cells.
Additional file 6. Effects of malaria on the remaining of CD34 + cells.
Additional file 7. Activation of naïve T lymphocytes by the lysate of P. falciparum infected erythrocytes.
Literatur
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