Background
Methods
Synthesis of naproxen derivatives (1–4)
Cell culture
MTT assay
Apoptosis assay
Caspase assay
Cell cycle study
PGE2synthesis assay
Cell migration assay
Statistical analysis
Results and discussion
Enhanced cytotoxic killing is evident by naproxen-derivatives against MCF-7 and MDA-MB-231 cells
24-h IC50, mM | ||
---|---|---|
Compound | MCF-7 | MDA-MB-231 |
ER+, PR+
| ER-, PR-, HER2/Neu-
| |
Naproxen sodium (NS) | >5 | ~10 |
1 | ~1.8 | ~1.6 |
2 | ~3 | ~3 |
3 | >10 | >10 |
4 | ~2.7 | ~5.9 |
Treatment with 4 induces early apoptosis in MDA-MB-231 cells
Naproxen derivative 4 induces caspase-3/9 mediated apoptosis in MDA-MB-231 cells
Neither NS nor 4 induces cell-cycle arrest in MDA-MB-231 cells
Naproxen derivative 4 inhibits PGE2synthesis in MDA-MB-231 cells
Treatment with 4 can slow-down cell-migration rate in MDA-MB-231cells
Drug Treatment (1 mM) | Migration speed (μm/h) |
---|---|
Control | ~12 |
NS | ~8.83 |
4 | ~5.3 |