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Erschienen in: Archives of Dermatological Research 2/2012

01.03.2012 | Original Paper

A putative in vitro organotypic model of molting with human skin explants

verfasst von: A. Peramo, S. E. Feinberg, C. L. Marcelo

Erschienen in: Archives of Dermatological Research | Ausgabe 2/2012

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Abstract

We report finding a simple method to partially reproduce the characteristic process of molting that takes place in invertebrates using human skin explants in vitro. In this method, human skin explants discarded from regular plastic surgery procedures were kept, submersed, in regular growth medium for 10 days at 4°C. After that period, the skin explants were cultured at the air–liquid interface for another 10 days. Histological analysis of the skin revealed the formation of one full epidermal structure and an additional intermediate epidermal structure containing a putative stratum corneum, superimposed one of top of the other, in which we consider an equivalent model of “molting” or “ecdysis”. Basic analysis of cell proliferation and differentiation of the explants at different stages of the process are briefly presented. We believe this model can be used in the study of certain human skin diseases as well as in comparative animal physiology.
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Metadaten
Titel
A putative in vitro organotypic model of molting with human skin explants
verfasst von
A. Peramo
S. E. Feinberg
C. L. Marcelo
Publikationsdatum
01.03.2012
Verlag
Springer-Verlag
Erschienen in
Archives of Dermatological Research / Ausgabe 2/2012
Print ISSN: 0340-3696
Elektronische ISSN: 1432-069X
DOI
https://doi.org/10.1007/s00403-011-1187-z

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