Background
Methods
Instruments and reagents
No. | Brand | Chonglou saponin VII | Chonglou saponin VI | Chonglou saponin II | Chonglou saponin I |
---|---|---|---|---|---|
col1 | Discovery C
18
a
| 21.234 ± 0.021 | 23.001 ± 0.006 | 32.773 ± 0.007 | 35.118 ± 0.004 |
col2 | Discovery C
18
b
| 16.555 ± 0.010 | 17.989 ± 0.006 | 27.161 ± 0.011 | 29.154 ± 0.002 |
col3 | Xbridge C
18
a
| 21.101 ± 0.004 | 23.070 ± 0.027 | 32.483 ± 0.021 | 34.963 ± 0.006 |
col4 | BDS HypersilC
18
a
| 21.014 ± 0.011 | 22.898 ± 0.018 | 32.679 ± 0.032 | 35.170 ± 0.036 |
col5 | Inertsil ODS-2a
| 22.132 ± 0.009 | 24.502 ± 0.004 | 33.176 ± 0.017 | 35.936 ± 0.003 |
col6 | Kromasil C
18
a
| 21.276 ± 0.012 | 23.693 ± 0.016 | 32.618 ± 0.006 | 35.929 ± 0.007 |
col7 | Luna C18(2)a
| 20.760 ± 0.018 | 23.362 ± 0.008 | 30.941 ± 0.011 | 33.813 ± 0.003 |
col8 | Luna C18(2)b
| 16.551 ± 0.008 | 18.865 ± 0.023 | 25.923 ± 0.016 | 28.310 ± 0.011 |
col9 | Inertsil ODS-3b
| 17.225 ± 0.006 | 19.640 ± 0.005 | 26.754 ± 0.013 | 29.471 ± 0.005 |
col10 | Alltima C
18
a
| 20.856 ± 0.008 | 23.752 ± 0.021 | 31.687 ± 0.011 | 34.872 ± 0.004 |
col11 | Symmetry C
18
a
| 21.016 ± 0.015 | 22.076 ± 0.016 | 32.470 ± 0.023 | 35.476 ± 0.010 |
col12 | Gemini C
18
a
| 21.300 ± 0.014 | 23.756 ± 0.007 | 31.599 ± 0.003 | 34.337 ± 0.015 |
col13 | CapcellpakC18MGa
| 21.076 ± 0.012 | 23.828 ± 0.004 | 31.627 ± 0.006 | 34.695 ± 0.001 |
col14 | Zorbax Extend-C
18
a
| 17.201 ± 0.022 | 19.731 ± 0.003 | 27.525 ± 0.009 | 30.504 ± 0.005 |
col15 | Sunfire C
18
a
| 21.652 ± 0.013 | 24.065 ± 0.024 | 32.375 ± 0.010 | 35.197 ± 0.006 |
col16 | Sunfire C
18
b
| 17.501 ± 0.006 | 19.571 ± 0.018 | 27.401 ± 0.004 | 29.826 ± 0.010 |
col17 | Nucleosil C18 HDa
| 21.452 ± 0.013 | 23.735 ± 0.006 | 32.747 ± 0.019 | 35.513 ± 0.011 |
col18 | ODS Hypersila
| 19.669 ± 0.010 | 21.583 ± 0.022 | 30.361 ± 0.007 | 32.657 ± 0.005 |
col19 | CapcellpakC18AQa
| 20.092 ± 0.013 | 22.560 ± 0.010 | 29.568 ± 0.006 | 32.218 ± 0.005 |
col20 | Spherisorb ODS2a
| 18.684 ± 0.006 | 21.334 ± 0.021 | 28.580 ± 0.008 | 31.385 ± 0.004 |
col21 | Zorbax SB-C
18
a
| 18.438 ± 0.015 | 20.986 ± 0.010 | 28.085 ± 0.008 | 30.849 ± 0.007 |
col22 | DiamonsilC
18
a
| 22.082 ± 0.021 | 25.078 ± 0.005 | 32.475 ± 0.020 | 35.721 ± 0.023 |
col23 | DiamonsilC
18
b
| 16.769 ± 0.007 | 19.154 ± 0.008 | 26.240 ± 0.011 | 28.935 ± 0.004 |
col24 | Diamonsil C18(2)a
| 19.296 ± 0.016 | 22.819 ± 0.004 | 31.192 ± 0.019 | 34.710 ± 0.009 |
col25 | Kromasil Eternity C
18
a
| 19.732 ± 0.017 | 22.099 ± 0.018 | 29.535 ± 0.004 | 32.153 ± 0.021 |
col26 | Shim-pack VP-ODSb
| 19.015 ± 0.007 | 21.059 ± 0.014 | 29.401 ± 0.002 | 31.845 ± 0.001 |
col27 | Agilent HC-C
18
a
| 23.492 ± 0.247 | 25.839 ± 0.250 | 34.884 ± 0.344 | 37.637 ± 0.330 |
col28 | Agilent TC-C
18
a
| 20.609 ± 0.003 | 22.574 ± 0.005 | 30.806 ± 0.003 | 32.783 ± 0.002 |
col29 | Venusil MP C
18
b
| 17.970 ± 0.007 | 20.468 ± 0.011 | 27.287 ± 0.031 | 29.985 ± 0.007 |
col30 | Nucleosil C18 ABa
| 18.348 ± 0.012 | 20.209 ± 0.017 | 29.233 ± 0.011 | 31.893 ± 0.014 |
StR (Average tR) | 19.803 | 22.110 | 30.319 | 33.035 |
Preparation of sample solution
Chromatographic conditions
Results
HPLC chromatogram of samples
Standard retention time (StR)
Linear principle of LCTRS
Minimum number of columns for StR calculation
Procedure of two points prediction
Peak 1 | Peak 2 | Peak 3 | Peak 4 | Peak 5 | Peak 6 | Peak 7 | Peak 8 | Peak 9 | Peak 10 | Peak 11 | |
---|---|---|---|---|---|---|---|---|---|---|---|
Psoraleae (n = 23) | 0.226 | 0.247 | 0.615 | 0.657 | 0.794 | 0.808 | 0.878 | 1.000a
| 1.061 | 1.103 | 1.152 |
Paridis (n = 30) | 0.652 | 0.729 | 1.000a
| 1.090 |
Peak 1 | Peak 2 | Peak 3 | Peak 4 | Peak 5 | Peak 6 | Peak 7 | Peak 8 | Peak 9 | Peak 10 | Peak 11 | |
---|---|---|---|---|---|---|---|---|---|---|---|
Psoraleae (n = 23) | 9.271 | 10.122a
| 25.143 | 26.854 | 32.437 | 32.951 | 35.843 | 40.793a
| 43.254 | 44.950 | 46.937 |
Paridis (n = 30) | 19.803a
| 22.156 | 30.319 | 33.035a
|
Procedure of multiple points regression
Sequential matching rule
Comparison between LCTRS method and RR method
Method | Maximum of ΔtR/min | Average of ΔtR/min | Number of positive columnsa
|
---|---|---|---|
Unadjusted RR | 3.494 | 0.804 | 2 |
Adjusted RR | 3.001 | 0.886 | 3 |
Prediction by two points | 2.194 | 0.599 | 5 |
Validation by multiple points | 1.689 | 0.465 | 9 |
Method | Maximum of ΔtR/min | Average of ΔtR/min | Number of positive columnsa
|
---|---|---|---|
Unadjusted RR | 1.811 | 0.420 | 12 |
Adjusted RR | 1.562 | 0.410 | 9 |
Prediction by two points | 0.836 | 0.283 | 25 |
Validation by multiple points | 0.545 | 0.204 | 30 |