Skip to main content
main-content

12.08.2016 | Original Article | Ausgabe 11/2016

Annals of Hematology 11/2016

A whole blood model of thrombocytopenia that controls platelet count and hematocrit

Zeitschrift:
Annals of Hematology > Ausgabe 11/2016
Autoren:
R. S. Bercovitz, M. K. Brenner, D. K. Newman
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s00277-016-2777-9) contains supplementary material, which is available to authorized users.

Abstract

In patients with thrombocytopenia, it can be difficult to predict a patient’s bleeding risk based on platelet count alone. Platelet reactivity may provide additional information; however, current clinical assays cannot reliably assess platelet function in the setting of thrombocytopenia. New methods to study platelet reactivity in thrombocytopenic samples are needed. In this study, we sought to develop a laboratory model of thrombocytopenia using blood from healthy subjects that preserves the whole blood environment and reproducibly produces samples with a specific platelet count and hematocrit. We compared the activation state of unstimulated and agonist-stimulated platelets in thrombocytopenic samples derived from this method with normocytic controls. Whole blood was diluted with autologous red blood cell concentrate and platelet-poor plasma, which were obtained via centrifugation, in specific ratios to attain a final sample with a predetermined platelet count and hematocrit. P-selectin exposure and GPIIbIIIa activation in unstimulated platelets and platelets stimulated with collagen-related peptide (CRP) or adenosine diphosphate (ADP) in thrombocytopenic samples and the normocytic control from which they were derived were quantified by flow cytometry. Our methodology reliably produced thrombocytopenic samples with a platelet count ≤50,000/μL and an accurately and precisely controlled hematocrit. P-selectin exposure and GPIIbIIIa activation on unstimulated platelets or on ADP- or CRP-stimulated platelets did not differ in thrombocytopenic samples compared to normocytic controls. We describe a new method for creating thrombocytopenic blood that can be used to better understand the contributions of platelet number and function to hemostasis.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Premium-Inhalten der Fachzeitschriften, inklusive eines Print-Abos.

Weitere Produktempfehlungen anzeigen
Zusatzmaterial
Supplemental Material 1 Description of how the equations to calculate volumes of whole blood, red cell concentrate, and platelet-poor plasma should be added together to get the reconstituted whole blood with the goal platelet count and hematocrit. (PDF 1675 kb)
277_2016_2777_MOESM1_ESM.pdf
Supplemental Material 2 Calculator to determine volumes of whole blood, RBCs, and PPP to use to achieve goal hematocrit and platelet count. (XLS 21 kb)
277_2016_2777_MOESM2_ESM.xls
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 11/2016

Annals of Hematology 11/2016Zur Ausgabe
  1. Das kostenlose Testabonnement läuft nach 14 Tagen automatisch und formlos aus. Dieses Abonnement kann nur einmal getestet werden.

  2. Das kostenlose Testabonnement läuft nach 14 Tagen automatisch und formlos aus. Dieses Abonnement kann nur einmal getestet werden.

Neu im Fachgebiet Onkologie

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Onkologie und bleiben Sie gut informiert – ganz bequem per eMail.

Bildnachweise