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Journal of Cancer Research and Clinical Oncology

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01.08.2011 | Original Paper

Aberrant promoter methylation and inactivation of PTEN gene in cervical carcinoma from Indian population

verfasst von: M. Moshahid Alam Rizvi, M. Shabbir Alam, Asgar Ali, Syed Jafar Mehdi, Swaraj Batra, A. K. Mandal

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 8/2011

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Abstract

Purpose

PTEN, a tumor-suppressor gene located on chromosome 10q23.3 is implicated in multiple tumors including cervical carcinoma.

Methods

We examined 135 cervical cancer specimens for PTEN gene expression and promoter methylation using methylation-specific PCR and immunohistochemistry and also studied the mutation in PTEN gene through PCR-single-stranded conformational polymorphism. PTEN expression and its methylation status were also correlated with clinicopathologic parameters.

Results

The results showed an abnormal band on exon 5 and exon 9 of the PTEN gene. In PTEN gene, 61% specimen showed methylation. PTEN methylation was found in 39% cases of stage I, 60% of stage II, and 75% of stages III–IV. The correlation between PTEN methylation and clinical stage was found to be statistically significant (P = 0.003). Nuclear PTEN expression was detected in 84 of 135 (62%) cases of cervical carcinoma, and the remaining 51 of 135 (38%) cases were observed as expressional loss. The loss of PTEN expression was significantly correlated clinical stage (P = 0.001). Loss of PTEN expression was observed in 34 (41%) cases among 83 methylation positive cases, whereas among 52 methylation-negative cases, only 13 (25%) cases were seen as immunostaining negative with the statistically significant value (P = 0.05).

Conclusion

Promoter methylation and loss of PTEN expression occur frequently in carcinoma of uterine cervix. Our results suggest that PTEN plays an important role in the carcinogenesis of cervical cancer.

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Titel: Aberrant promoter methylation and inactivation of PTEN gene in cervical carcinoma from Indian population
verfasst von: M. Moshahid Alam Rizvi
M. Shabbir Alam
Asgar Ali
Syed Jafar Mehdi
Swaraj Batra
A. K. Mandal
Publikationsdatum: 01.08.2011
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 8/2011
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-011-0994-0

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Aberrant promoter methylation and inactivation of PTEN gene in cervical carcinoma from Indian population

Abstract

Purpose

Methods

Results

Conclusion

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

Purpose

Methods

Results

Conclusion

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