Rainer Beckmann Sebastian Lippross Claudia Hartz Mersedeh Tohidnezhad Thomas Pufe and Deike J. Varoga contributed equally to this work.
The authors declare that they have no competing interests.
All authors were involved in drafting the article or revising it critically for important intellectual content, and all authors read and approved the final version to be published. Conception and design: CH, HJ, BR, SL, TP, DV. Collection and assembly of data: RB, CH, AS, MT. Analysis and interpretation of the data: RB, CH, HJ, MT, SL, SNS, BR, TP, DV, MVF. Drafting and revising of the article: RB, CH, HJ, SL, SNS, MT, TP, AS, DV, MVF, SN, BR, NK.
Abrasion arthroplasty (AAP) is a procedure by which intrinsic cartilage healing is believed to be stimulated. Although clinically accepted for degenerative and traumatic cartilage lesions scientific evidence at a molecular level that proves the effect of AAP is scarce.
Mononuclear cells were extracted from postoperative joint effusions 21.5 h post AAP and simple debridement of cartilage lesions. Luminex, ELISA and FACS experiments were performed. Immunohistochemical stainings of cell cultures for cartilage markers were used to confirm the findings.
Postoperative joint effusions after AAP showed increased contents of Mononuclear cells compared to Arthroscopic Chondroplasty (ACP). BMP-4 and IGF were increased in AAP as complared to ACP. Mononuclear cells isolated after AAP express the MSC markers CD 73, CD 105, CD 90, CD 44 and are CD34 negative. Chondrogenic differentiation was demonstrated by positive staining for Sox9, collagen II, proteoglycan, chondroitin-4-sulfate.
Our results support the clinical application of AAP as a procedure that enhances cartilage repair as an alternative to far more complex procedures that have gained popularity. Furthermore the data presented supports clinical investigations that recommend not to use suction drainage as by this procedure a considerable amount of the regeneratory potential of postoperative joint effusions might be extracted.
Ramappa AJ, Gill TJ, Bradford CH, Ho CP, Steadman JR. Magnetic resonance imaging to assess knee cartilage repair tissue after microfracture of chondral defects. J Knee Surg. 2007;20(3):228–34. PubMed
Kasten P, Beyen I, Egermann M, Suda AJ, Moghaddam AA, Zimmermann G, et al. Instant stem cell therapy: characterization and concentration of human mesenchymal stem cells in vitro. Eur Cell Mater. 2008;16:47–55. PubMed
Nakamura T, Sekiya I, Muneta T, Hatsushika D, Horie M, Tsuji K, et al. Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs. Cytotherapy. 2012;14(3):327–38. CrossRefPubMedPubMedCentral
Steinert AF, Proffen B, Kunz M, Hendrich C, Ghivizzani SC, Noth U, et al. Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer. Arthritis Res Ther. 2009;11(5):R148. CrossRefPubMedPubMedCentral
Shen B, Wei A, Whittaker S, Williams LA, Tao H, Ma DD, et al. The role of BMP-7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro. J Cell Biochem. 2010;109(2):406–16. PubMed
Cameron ML, Briggs KK, Steadman JR. Reproducibility and reliability of the outerbridge classification for grading chondral lesions of the knee arthroscopically. Am J Sports Med. 2003;31(1):83–6. PubMed
Gobbi A, Karnatzikos G, Kumar A. Long-term results after microfracture treatment for full-thickness knee chondral lesions in athletes. Knee Surg Sports Traumatol Arthrosc. 2013.
- Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions
Mónica S. Ventura Ferreira
Deike J. Varoga
- BioMed Central
Neu im Fachgebiet Orthopädie und Unfallchirurgie
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