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28.07.2017 | Original Article

Acid-gastric antisecretory effect of the ethanolic extract from Arctium lappa L. root: role of H⁺, K⁺-ATPase, Ca²⁺ influx and the cholinergic pathway

verfasst von: Luisa Mota da Silva, Ligia de Moura Burci, Sandra Crestani, Priscila de Souza, Rita de Cássia Melo Vilhena de Andrade Fonseca da Silva, Nessana Dartora, Lauro Mera de Souza, Thales Ricardo Cipriani, José Eduardo da Silva-Santos, Eunice André, Maria Fernanda de Paula Werner

Erschienen in: Inflammopharmacology | Ausgabe 2/2018

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Abstract

Background

Arctium lappa L., popularly known as burdock, is a medicinal plant used worldwide. The antiulcer and gastric-acid antisecretory effects of ethanolic extract from roots of Arctium lappa (EET) were already demonstrated. However, the mechanism by which the extract reduces the gastric acid secretion remains unclear. Therefore, this study was designed to evaluate the antisecretory mode of action of EET.

Materials and methods

The effects of EET on H⁺, K⁺-ATPase activity were verified in vitro, whereas the effects of the extract on cholinergic-, histaminergic- or gastrinergic-acid gastric stimulation were assessed in vivo on stimulated pylorus ligated rats. Moreover, ex vivo contractility studies on gastric muscle strips from rats were also employed.

Results

The incubation with EET (1000 µg/ml) partially inhibited H⁺, K⁺-ATPase activity, and the intraduodenal administration of EET (10 mg/kg) decreased the volume and acidity of gastric secretion stimulated by bethanechol, histamine, and pentagastrin. EET (100–1000 µg/ml) did not alter the gastric relaxation induced by histamine but decreased acetylcholine-induced contraction in gastric fundus strips. Interestingly, EET also reduced the increase in the gastric muscle tone induced by 40 mM KCl depolarizing solution, as well as the maximum contractile responses evoked by CaCl₂ in Ca²⁺-free depolarizing solution, without impairing the effect of acetylcholine on fundus strips maintained in Ca²⁺ -free nutritive solution.

Conclusion

Our results reinforce the gastric antisecretory properties of preparations obtained from Arctium lappa, and indicate that the mechanisms involved in EET antisecretory effects include a moderate reduction of the H⁺, K⁺-ATPase activity associated with inhibitory effects on calcium influx and of cholinergic pathways in the stomach muscle.

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Titel: Acid-gastric antisecretory effect of the ethanolic extract from Arctium lappa L. root: role of H+, K+-ATPase, Ca2+ influx and the cholinergic pathway
verfasst von: Luisa Mota da Silva
Ligia de Moura Burci
Sandra Crestani
Priscila de Souza
Rita de Cássia Melo Vilhena de Andrade Fonseca da Silva
Nessana Dartora
Lauro Mera de Souza
Thales Ricardo Cipriani
José Eduardo da Silva-Santos
Eunice André
Maria Fernanda de Paula Werner
Publikationsdatum: 28.07.2017
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 2/2018
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0378-4

Springer Medizin

Abstract

Background

Materials and methods

Results

Conclusion

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