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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Chuchard Punsawad, Srivicha Krudsood, Yaowapa Maneerat, Urai Chaisri, Noppadon Tangpukdee, Emsri Pongponratn, Kwannan Nantavisai, Rachanee Udomsangpetch, Parnpen Viriyavejakul
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-191) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

CP carried out the ELISA and immunofluorescence work, preliminary data analysis, and wrote the first draft of the manuscript. SK, NT and KN collected clinical data, supervised patient recruitment, critically analysed, and interpreted the data. YM, UC, EP and RU participated in the study design and manuscript preparation and revision. PV formulated the research idea, designed the experiments, gave laboratory and technical support, supervised, and revised the manuscript. All authors have approved the final version of the manuscript.

Abstract

Background

Malaria parasites and their products can activate a specific immune response by stimulating cytokine production in the host’s immune cells. Transcription nuclear factor kappa B (NF-κB) is an important regulator for the control of many pro-inflammatory genes, such as interleukin-1 (IL-1) and tumor necrosis factor (TNF). The activation and expression of NF-κB p65 in peripheral blood mononuclear cells (PBMCs) of malaria patients were investigated and correlated with the levels of IL-10 and TNF to study the nature of NF-κB p65 and its linkage to inflammatory cytokines.

Methods

The sample group comprised 33 patients admitted with malaria caused by Plasmodium vivax (n = 11), uncomplicated Plasmodium falciparum (n = 11), and complicated Plasmodium falciparum (n = 11). Peripheral blood was collected at admission and on day 7 for PBMC isolation. Healthy subjects were used as a control group. The expressions of NF-κB p65 in the PBMCs from malaria patients and the plasma levels of IL-10 and TNF were measured by using enzyme-linked immunosorbent assay (ELISA). The immunofluorescence technique was used to determine NF-κB nuclear translocation.

Results

At admission, patients with P. vivax and uncomplicated P. falciparum had significantly elevated phospho-NF-κB p65 levels in the PBMCs compared with those of healthy controls. However, patients with complicated P. falciparum malaria had decreased levels of phospho-NF-κB p65. On day 7 post-treatment, significantly increased phospho-NF-κB p65 was found in the PBMCs of patients with complicated P. falciparum, compared with healthy controls. The plasma level of IL-10 was elevated in day 0 in patients with complicated P. falciparum malaria and was found to be negatively correlated with phospho-NF-κB p65 level (r s  = −0.630, p = 0.038). However, there was no correlation between phospho-NF-κB p65 expression and TNF level in patients with complicated P. falciparum malaria.

Conclusions

This is the first report demonstrating alterations in NF-κB p65 activity in the PBMCs of malaria patients. The altered lower features of NF-κB p65 in the PBMCs of patients with complicated P. falciparum at admission could be due to a suppressive effect of high IL-10 associated with complicated P. falciparum malaria.
Zusatzmaterial
Authors’ original file for figure 1
12936_2012_2145_MOESM1_ESM.pdf
Authors’ original file for figure 2
12936_2012_2145_MOESM2_ESM.pdf
Authors’ original file for figure 3
12936_2012_2145_MOESM3_ESM.pdf
Authors’ original file for figure 4
12936_2012_2145_MOESM4_ESM.pdf
Literatur
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