Background
Infectious mononucleosis (IM) is caused by acute Epstein-Barr virus (EBV) infection and presents with a classical triad of fever, pharyngitis, and lymphadenopathy [
1]. It occurs mostly in children, adolescents, and young adults. In European and American countries, it mainly affects adolescents and adults aged 10–30 years [
2]; however, in China, it usually occurs in children aged 4–6 years [
3]. An elevated transaminase level is one of the most common features of IM. Several studies [
4‐
6] have shown that transaminase levels are elevated in up to 80–90% of cases, while jaundice is seen in about 5% of cases. There are also rare cases where severe hepatitis or acute hepatic failure develop [
6,
7].
Adenosine deaminase (ADA) is a purine metabolism enzyme encoded by the ADA gene. ADA mutations can lead to severe combined immune deficiency [
8]. A large number of studies have confirmed that ADA plays an important role in the growth and differentiation of lymphocytes and macrophages, and is considered a marker of T lymphocyte-mediated cellular immunity [
9]. Elevated ADA levels can be observed in autoimmune diseases, acquired immune deficiency syndrome, and tumors, and its level is closely related to the severity of these diseases [
10,
11]. Previous research has shown that ADA levels can predict disease severity in individuals with hepatitis B, hepatitis C, and autoimmune hepatitis [
12]. Although ADA is known to be associated with numerous diseases, its utility in assessing disease severity in pediatric patients with IM has not been determined.
The aim of this study was to explore the pathophysiology of ADA in children with IM and concurrently elevated ALT, to assess whether ADA measurement is useful for assisting clinicians in the timely diagnosis of IM and judgment of disease severity.
Materials and methods
Patient characteristics
This case-control study was conducted at the Children’s Hospital of Soochow University. Children with a disease onset > 5 days previously, mixed infections, autoimmune diseases and chronic diseases were excluded. The criteria of IM were as follows [
12]: (1) presence of at least three of the following clinical manifestations: fever, pharyngitis, cervical lymphadenopathy, splenomegaly, eyelid edema and hepatomegaly; (2) IgM to EBV viral capsid antigen (VCA-IgM) and IgG to EBV capsid antigen (VCA-IgG) positive, with absence of the antibody to EB nuclear antigen (EBNA); and (3) exclusion of other viral infections such as human immunodeficiency virus, cytomegalovirus, hepatotropic virus and herpes simplex virus. The inclusion criteria of the control group were negative EBV-specific antibody and plasma EBV-DNA polymerase chain reaction (PCR) test results, disease onset < 5 days previously, and no history of chronic infectious diseases, immune system diseases and use of immunomodulators in the past 14 days. On admission to the hospital, the blood samples were collected for testing.
Routine complete blood count, alanine transaminase, adenosine deaminase and immunoglobulin G, M, and A assays
Routine blood count of venous blood from participants was performed on the type BC-5310 instrument (Shenzhen Mindray Biomedical Electronics Co., Ltd). Serum ALT and ADA levels were measured using a lactate dehydrogenase assay (Beijing Strong Biotechnologies, Inc.) and peroxidase assay (test kit from Meikang Biotechnology Co., Ltd), respectively. Both were detected using a HITACHI 7180 biomedical analyzer. Immunoglobulin G (IgG), M (IgM), and A (IgA) were detected using a turbidimetric inhibition immunoassay. Anti-human IgG/IgM/IgA antibody (Orion Diagnostica Oy) was added to a certain proportion of the sample and buffer, which produced an agglutination reaction with IgG/IgM/IgA in the sample, resulting in an increase in the turbidity of the mixture. A Konelab clinical chemistry analyzer was used to detect turbidity at 340 nm wavelength.
Flow cytometry
Lymphocyte subsets, including T cells (CD3+), helper T cells (CD3+CD4+), killer T cells (CD3+CD8+), natural killer cells (CD3−CD (16+56)+), B cells (CD3−CD19+), and activated B cells (CD19+CD23+) were detected using flow cytometry. Peripheral whole blood samples were labeled with antibodies including anti-CD3-fluorescein isothiocyanate, anti-CD16+56− phycoerythrin, anti-CD45-peridin chlorophyll alpha protein-cyanin5.5, anti-CD4-phycoerythrin cyanin 7, anti-CD19-APC, anti-CD23-Fc EpsilonR II, and anti-CD8-allophycocyanin-cyanin7. The samples were centrifuged and kept in the dark at room temperature for 15 min. Each sample was analyzed using a multi-color flow cytometer (BD FACSCanto II) according to the manufacturer’s instructions.
Indirect immunofluorescence assay (IIF) for EBV-specific antibodies
The anti-EBV-VCA IgG /IgM, anti-EBV-early antigen (EA) IgG and anti-EBNA IgG IIF kits (EUROIMMUN, Lübeck, Germany) was used for testing the EBV-VCA, EA and EBNA in serum. All steps were carried out according to the manufacturer’s instructions. The antibody affinity was determined by comparing the serum fluorescence intensities of children treated with and without urea treatment. The difference in fluorescence intensity was ≥ 2 for low affinity, and < 2 for high affinity.
Plasma Epstein-Barr virus-DNA polymerase chain reaction assay
Plasma EB viral load was determined by polymerase chain reaction, and EBV nucleic acid quantitative detection kit came from Shengxiang Biotechnology Co., Ltd, Hunan, China. Amplification and detection were performed on the LightCycler 480II instrument (Roche, Basel, Switzerland) following the manufacturer’s instructions. Copy numbers were calculated by comparing the cycle threshold (Ct) of the specimens to the standard curve. EBV positivity was defined as a Ct value ≤ 39 (DNA copy number > 400 copies/mL).
All peripheral blood samples were collected within 24 h of admission and sent to the laboratory for analysis immediately.
Statistical analysis
The Shapiro-Wilk normality test was used to determine whether continuous variables were normally distributed. Values were expressed as the mean ± standard deviation or median and interquartile range. The Mann-Whitney U-test and Kruskal-Wallis test were used for non-normally distributed data. Student’s t-test and analysis of variance (ANOVA) were used for normally distributed variables. Categorical variables were reported as frequency (%) and the frequency in different groups was compared using Chi-squared test or Fisher’s exact test. Spearman correlation analysis was used to determine the correlation between discrete variables. Logistic regression analysis was used to determine odds ratios (ORs) with 95% confidence intervals (CIs). Receiver-operating characteristic (ROC) curve analysis was used to assess the diagnostic accuracy of adenosine deaminase in children with IM. The cut-off value for ADA was determined using Youden’s index. All statistical analyses were performed using SPSS version 25.0 (IBM Corp., Armonk, NY, USA). P-values < 0.05 were considered to be statistically significant.
Discussion
EBV is prevalent worldwide. The symptoms of EBV infection in children are often atypical [
13], and the diagnosis relies mainly on serological assays [
14]. However, due to the immature immune system of children and the delay in developing IgM antibodies, it is easy clinically misdiagnose IM in children and to miss the diagnosis. In this study, ADA was significantly higher in children with IM than in those with acute infectious diseases caused by other pathogens, and it has a high diagnostic accuracy for identifying children with IM. ADA also has a high predictive value for predicting which children had high ALT in the early stages of IM, which is often asymptomatic. Therefore, ADA can not only help clinicians diagnose IM early, but also indirectly reflect the severity of the disease.
IM is caused by acute EBV infection and presents with a classical triad of fever, pharyngitis, and lymphadenopathy [
1], which are difficult to distinguish from febrile diseases caused by other pathogens. Previous studies [
15,
16] have confirmed that the absolute lymphocyte count is significantly elevated in children with IM, especially CD8 + T cells, and that the CD4/CD8 ratio becomes inverted. This is consistent with the results of this study. In addition, this study found that the incidence of cervical lymphadenopathy, hepatomegaly, splenomegaly, and puffy eyelids, and the levels of immunoglobulins (IgA, IgG, and IgM) in children with IM was higher than that in children with infectious diseases caused by other pathogens.
Previous studies have shown that ADA plays an important role in the growth and differentiation of lymphocytes and macrophages [
9], and it was elevated in children with IM, but its mechanism has not been described [
17]. This study also found that ADA levels were significantly increased in children with IM, and that there was a high correlation between the ADA level and the absolute lymphocyte count.
ROC curve analysis showed that ADA had a high diagnostic accuracy for distinguishing children with IM from children with acute febrile diseases caused by other pathogens. The most common feature of infectious mononucleosis was elevated transaminases [
4‐
6]. In this study, there was no difference in clinical manifestations of IM between children with elevated and normal ALT levels, but the viral load, ADA, CD8
+ T cell (%), and IgG level were higher in children with elevated ALT levels. Previous studies have found that the absolute lymphocyte count is an indicator of Epstein-Barr virus-related liver damage [
18], and that the EBV-DNA copy number is directly proportional to the severity of IM [
19].
The mechanism of EBV hepatitis is thought to be that EBV infected and activated CD8 + T-cells accumulate in the liver and the products of the EBV-infected CD8 + T cells or infiltrating cytotoxic T lymphocytes were interferon-γ, tumor necrosis factor and Fas ligand, which can destroy hepatocytes [
20‐
23]. In this study, the ADA level was closely related to the ALT level and had a good predictive value for ALT in the children with IM. In addition, it had a high diagnostic value for IM even when EBV-related antibodies were not produced in the early stage. Therefore, ADA could play a useful role in the diagnosis of IM and the prediction of the severity of the disease in the early stages of the disease.
Our study has several limitations. First, liver biopsy, to better characterize the liver pathology in patients with IM was lacking. Second, we only studied ADA and ALT at a single point in time early in the course of disease.
Conclusions
ADA had a high diagnostic accuracy for identifying IM in children with acute febrile diseases. ADA was highly correlated with the lymphocyte count in children with IM, especially CD8 + T cells. In addition, the ADA level indirectly reflected the severity of IM in children in the early stages, especially those with elevated ALT levels. These results reveal that measuring ADA in children with suspected IM can play a useful role in helping clinicians diagnose IM early and in predicting the severity of the disease.
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