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01.06.2015 | Experimental Study | Ausgabe 3/2015

European Journal of Plastic Surgery 3/2015

Adipocyte and preadipocyte viability in autologous fat grafts: comparing the water jet-assisted liposuction (WAL) and Coleman techniques

Zeitschrift:
European Journal of Plastic Surgery > Ausgabe 3/2015
Autoren:
Hui-Ling Chia, Evan Woo, Yong-Chen Por, Dong-Rui Ma, Kenneth Chang, James Mok, Jonah Kua, Vincent Yeow

Abstract

Background

Autologous fat transplantation is a promising technique for soft tissue augmentation. However, the long-term maintenance of fat grafts remains unpredictable. Based on Peer’s cell theory, techniques that cause less cellular damage will optimize graft integration. Water jet-assisted liposuction (WAL) was introduced as a gentle and efficient technique for harvesting a large volume of fat in a short period of time. In this study, we evaluated the viability and function of adipocytes and preadipocytes harvested using WAL and compared this with the Coleman technique.

Methods

Eleven patients were enrolled in this study. Fat grafts were harvested using WAL from one limb and the Coleman technique from the other limb. The lipoaspirates were compared based on the following analyses: trypan blue vital staining for viable adipocyte and preadipocyte counts, glycerol-3-phophatase dehydrogenase assay for adipocyte function, histological examination, and speed of fat harvest.

Results

Viable adipocyte and preadipocyte counts were significantly higher in the Coleman group. The level of G3PDH activity was also significantly higher in the Coleman group, indicating that adipocytes within the harvested fat have improved cellular function. Histological findings were comparable between the two techniques, showing mild to moderate architectural disruption and adipocyte degeneration, with no evidence of tissue necrosis. Time taken for fat aspiration was significantly shorter using WAL.

Conclusions

This study demonstrated that the Coleman technique yields a greater number of viable adipocytes and preadipocytes. Although the histologic structure of fat grafts was comparable between the two techniques, the cellular function of adipocytes was higher for fat grafts harvested using the Coleman technique. Nonetheless, for cases where large volume of fat graft is required, the WAL technique may be preferred for its efficiency in fat harvesting.
Level of Evidence: Not ratable

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