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09.10.2018 | Original Article

Adipocyte proteome and secretome influence inflammatory and hormone pathways in glioma

verfasst von: J. Almeida, J. Costa, P. Coelho, V. Cea, M. Galesio, J. P. Noronha, M. S. Diniz, C. Prudêncio, R. Soares, C. Sala, Rúben Fernandes

Erschienen in: Metabolic Brain Disease | Ausgabe 1/2019

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Abstract

Gliomas represent the most common primary malignant brain tumors in adults, with an extremely poor prognosis. Among several risk factors, lifestyle was also recently identified as a major risk factor for the development of primary glioma. In the present study, we explore the relationship between obesity and glioma in a cellular model. Thus, we have study the influence of adipocytes secretome on glioma cell line GL261. Using the 3T3-L1 adipocyte cell line, and its conditioned medium (adipokines-enriched medium), we showed that adipocyte-released factors relate with glioma angiogenic, growth, hormones and metabolic behavior by MALDI-TOF-MS and proteomic array analysis. In a first view, STI1, hnRNPs and PGK1 are under expressed on CGl. Similarly, both carbonic anhydrase and aldose reductase are even suppressed in glioma cells that grown under adipokines-enriched environment. Contrariwise, RFC1, KIF5C, ANXA2, N-RAP and RACK1 are overexpressed in GL261 cell the in the presence of the adipokines-enriched medium. We further identified the factors that are released by adipocyte cells, and revealed that several pro-inflammatory and angiogenic factors, such as IL-6, IL-11, LIF, PAI-1, TNF-α, endocan, HGF, VEGF IGF-I, were secreted to the medium into a high extent, whereas TIMP-1 and SerpinE1 were under expressed on CGl. This study discloses an interesting in vitro model for the study of glioma biology under a “obesity” environment, that can be explored for the understanding of cancer cells biology, for the search of biomarkers, prognostic markers and therapeutic approaches.

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Titel: Adipocyte proteome and secretome influence inflammatory and hormone pathways in glioma
verfasst von: J. Almeida
J. Costa
P. Coelho
V. Cea
M. Galesio
J. P. Noronha
M. S. Diniz
C. Prudêncio
R. Soares
C. Sala
Rúben Fernandes
Publikationsdatum: 09.10.2018
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 1/2019
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-018-0327-y

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Adipocyte proteome and secretome influence inflammatory and hormone pathways in glioma

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