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26.05.2020 | Basic Science • Original Article

Adiponectin ameliorates lung injury induced by intermittent hypoxia through inhibition of ROS-associated pulmonary cell apoptosis

verfasst von: Wenxiao Ding, Xilong Zhang, Qiang Zhang, Yanbin Dong, Wenjing Wang, Ning Ding

Erschienen in: Sleep and Breathing | Ausgabe 1/2021

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Abstract

Purpose

Obstructive sleep apnea hypopnea syndrome has been reported to be associated with pulmonary hypertension (PH). Adiponectin (Ad) has many protective roles in the human body, including its function as an anti-inflammatory and an anti-oxidant, as well as its role in preventing insulin resistance and atherosclerosis. This study aimed to investigate the molecular mechanism of chronic intermittent hypoxia (CIH)-induced pulmonary injury and the protective role of Ad in experimental rats.

Methods

Thirty male Sprague-Dawley rats were randomly divided into three groups with 10 rats in each group: normal control (NC) group, CIH group, and CIH + Ad group. Rats in the NC group were kept breathing room air for 12 weeks. Rats in the CIH group were intermittently exposed to a hypoxic environment for 8 h/day for 12 weeks. Rats in the CIH + Ad group received 10 μg Ad twice weekly via intravenous injection. After 12 weeks of CIH exposure, we detected the pulmonary function, pulmonary artery pressure, lung histology, pulmonary cell apoptosis, pulmonary artery endothelial cell apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) level. We also analyzed expression proteins involved in the mitochondria-, endoplasmic reticulum (ER) stress-, and Fas receptor-associated pulmonary apoptosis pathways, as well as the SIRT3/SOD2 pathway.

Results

CIH exposure for 12 weeks did not lead to abnormal pulmonary function, PH, or pulmonary artery endothelial cell apoptosis. However, we observed a significant increase in the rate of pulmonary cell apoptosis, the expression of proteins involved in mitochondria-, ER stress-, and Fas receptor-associated pulmonary apoptosis pathways, and the generation of ROS in the CIH group compared with the NC group. In contrast, the MMP and protein expressions of SIRT3/SOD2 pathway were significantly decreased in the CIH group compared with the NC group. Ad supplementation in the CIH + Ad group partially improved these changes induced by CIH.

Conclusion

Even though CIH did not cause abnormal pulmonary function or PH, early lung injury was detected at the molecular level in rats exposed to CIH. Treatment with Ad ameliorated the pulmonary injury by activating the SIRT3/SOD2 pathway, reducing ROS generation, and inhibiting ROS-associated lung cell apoptosis.

Feldstein CA (2016) Blood pressure effects of CPAP in nonresistant and resistant hypertension associated with OSA: a systematic review of randomized clinical trials. Clin Exp Hypertens 38(4):337–346. https://doi.org/10.3109/10641963.2016.1148156 CrossRefPubMed

Khan F, Walsh C, Lane SJ, Moloney E (2014) Sleep apnoea and its relationship with cardiovascular, pulmonary, metabolic and other morbidities. Ir Med J 107(1):6–8 PubMed

Rosenkranz S, Gibbs JS, Wachter R, De Marco T, Vonk-Noordegraaf A, Vachiery JL (2016) Left ventricular heart failure and pulmonary hypertension. Eur Heart J 37(12):942–954. https://doi.org/10.1093/eurheartj/ehv512 CrossRefPubMed

Wong HS, Williams AJ, Mok Y (2017) The relationship between pulmonary hypertension and obstructive sleep apnea. Curr Opin Pulm Med 23(6):517–521. https://doi.org/10.1097/MCP.0000000000000421 CrossRefPubMed

Wong HT, Chee KH, Chong AW (2017) Pulmonary hypertension and echocardiogram parameters in obstructive sleep apnea. Eur Arch Otorhinolaryngol 274(6):2601–2606. https://doi.org/10.1007/s00405-017-4491-1 CrossRefPubMed

Fagan KA (2001) Selected contribution: pulmonary hypertension in mice following intermittent hypoxia. J Appl Physiol 90(6):2502–2507 CrossRef

Hamada S, Sato A, Hara-Chikuma M, Satooka H, Hasegawa K, Tanimura K, Tanizawa K, Inouchi M, Handa T, Oga T, Muro S, Mishima M, Chin K (2016) Role of mitochondrial hydrogen peroxide induced by intermittent hypoxia in airway epithelial wound repair in vitro. Exp Cell Res 344:143–151. https://doi.org/10.1016/j.yexcr.2016.04.006 CrossRefPubMed

Pelster B, Wood CM, Jung E, Val AL (2018) Air-breathing behavior, oxygen concentrations, and ROS defense in the swimbladders of two erythrinid fish, the facultative air-breathing jeju, and the non-air-breathing traira during normoxia, hypoxia and hyperoxia. J Comp Physiol B 188(3):437–449. https://doi.org/10.1007/s00360-017-1142-1 CrossRefPubMedPubMedCentral

Katwal G, Baral D, Fan X, Weiyang H, Zhang X, Ling L, Xiong Y, Ye Q, Wang Y (2018) SIRT3 a major player in attenuation of hepatic ischemia-reperfusion injury by reducing ROS via its downstream mediators: SOD2, CYP-D, and HIF-1alpha. Oxidative Med Cell Longev 2018:2976957–2976913. https://doi.org/10.1155/2018/2976957 CrossRef

10.

Ding W, Zhang Q, Dong Y, Ding N, Huang H, Zhu X, Hutchinson S, Gao X, Zhang X (2016) Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury through AMP-activated protein kinase pathway. Sci Rep 6:34151. https://doi.org/10.1038/srep34151 CrossRefPubMedPubMedCentral

11.

Fisman EZ, Tenenbaum A (2014) Adiponectin: a manifold therapeutic target for metabolic syndrome, diabetes, and coronary disease? Cardiovasc Diabetol 13:103. https://doi.org/10.1186/1475-2840-13-103 CrossRefPubMedPubMedCentral

12.

Van Berendoncks AM, Garnier A, Ventura-Clapier R, Conraads VM (2012) Adiponectin: key role and potential target to reverse energy wasting in chronic heart failure. Heart Fail Rev 18:557–566. https://doi.org/10.1007/s10741-012-9349-4 CrossRef

13.

Park M, Sweeney G (2012) Direct effects of adipokines on the heart: focus on adiponectin. Heart Fail Rev 18:631–644. https://doi.org/10.1007/s10741-012-9337-8 CrossRef

14.

Al Mutairi S, Mojiminiyi OA, Al Alawi A, Al Rammah T, Abdella N (2014) Study of leptin and adiponectin as disease markers in subjects with obstructive sleep apnea. Dis Markers 2014:706314–706318. https://doi.org/10.1155/2014/706314 CrossRefPubMedPubMedCentral

15.

Kim Y, Cho JY, Oh SW, Kang M, Lee SE, Jung E, Park YS, Lee J (2018) Globular adiponectin acts as a melanogenic signal in human epidermal melanocytes. Br J Dermatol 179(3):689–701. https://doi.org/10.1111/bjd.16488 CrossRefPubMed

16.

Kim EH, Park PH (2018) Globular adiponectin protects rat hepatocytes against acetaminophen-induced cell death via modulation of the inflammasome activation and ER stress: critical role of autophagy induction. Biochem Pharmacol 154:278–292. https://doi.org/10.1016/j.bcp.2018.05.014 CrossRefPubMed

17.

Ding W, Cai Y, Wang W, Ji L, Dong Y, Zhang X, Su M, Liu J, Lu G, Zhang X (2016) Adiponectin protects the kidney against chronic intermittent hypoxia-induced injury through inhibiting endoplasmic reticulum stress. Sleep Breath 20:1069–1074. https://doi.org/10.1007/s11325-016-1321-4 CrossRefPubMed

18.

Ding W, Zhang X, Huang H, Ding N, Zhang S, Hutchinson SZ, Zhang X (2014) Adiponectin protects rat myocardium against chronic intermittent hypoxia-induced injury via inhibition of endoplasmic reticulum stress. PLoS One 9(4):e94545. https://doi.org/10.1371/journal.pone.0094545 CrossRefPubMedPubMedCentral

19.

Tao R, Vassilopoulos A, Parisiadou L, Yan Y, Gius D (2014) Regulation of MnSOD enzymatic activity by Sirt3 connects the mitochondrial acetylome signaling networks to aging and carcinogenesis. Antioxid Redox Signal 20(10):1646–1654. https://doi.org/10.1089/ars.2013.5482 CrossRefPubMedPubMedCentral

20.

Celec P, Jurkovicova I, Buchta R, Bartik I, Gardlik R, Palffy R, Mucska I, Hodosy J (2013) Antioxidant vitamins prevent oxidative and carbonyl stress in an animal model of obstructive sleep apnea. Sleep Breath 17(2):867–871. https://doi.org/10.1007/s11325-012-0728-9 CrossRefPubMed

21.

Nara A, Nagai H, Shintani-Ishida K, Ogura S, Shimosawa T, Kuwahira I, Shirai M, Yoshida K (2015) Pulmonary arterial hypertension in rats due to age-related arginase activation in intermittent hypoxia. Am J Respir Cell Mol Biol 53(2):184–192. https://doi.org/10.1165/rcmb.2014-0163OC CrossRefPubMed

22.

Yao R, Zhou Y, He Y, Jiang Y, Liu P, Ye L, Zheng Z, Lau WB, Cao Y, Zeng Z (2015) Adiponectin protects against paraquat-induced lung injury by attenuating oxidative/nitrative stress. Exp Ther Med 9(1):131–136. https://doi.org/10.3892/etm.2014.2073 CrossRefPubMed

23.

Li PC, Wang BR, Li CC, Lu X, Qian WS, Li YJ, Jin FG, Mu DG (2018) Seawater inhalation induces acute lung injury via ROS generation and the endoplasmic reticulum stress pathway. Int J Mol Med 41(5):2505–2516. https://doi.org/10.3892/ijmm.2018.3486 CrossRefPubMedPubMedCentral

24.

Xu Y, Wang W, Jin K, Zhu Q, Lin H, Xie M, Wang D (2017) Perillyl alcohol protects human renal tubular epithelial cells from hypoxia/reoxygenation injury via inhibition of ROS, endoplasmic reticulum stress and activation of PI3K/Akt/eNOS pathway. Biomed Pharmacother 95:662–669. https://doi.org/10.1016/j.biopha.2017.08.129 CrossRefPubMed

25.

Kou Y, Zhang P, Wang H, Zhang J, Han X, Yu J, Wang L, Zhang M (2015) Changes of angiotensin II and oxidation stress during the development of chronic intermittent-induced pulmonary injury in rats. Zhonghua Jie He He Hu Xi Za Zhi 38(8):612–616 PubMed

26.

Liu W, Yang T, Xu Z, Xu B, Deng Y (2019) Methyl-mercury induces apoptosis through ROS-mediated endoplasmic reticulum stress and mitochondrial apoptosis pathways activation in rat cortical neurons. Free Radic Res 53(1):26–44. https://doi.org/10.1080/10715762.2018.1546852 CrossRefPubMed

27.

Liu XR, Li T, Cao L, Yu YY, Chen LL, Fan XH, Yang BB, Tan XQ (2018) Dexmedetomidine attenuates H2O2-induced neonatal rat cardiomyocytes apoptosis through mitochondria- and ER-medicated oxidative stress pathways. Mol Med Rep 17(5):7258–7264. https://doi.org/10.3892/mmr.2018.8751 CrossRefPubMedPubMedCentral

28.

Ma Y, Zhang J, Zhang Q, Chen P, Song J, Yu S, Liu H, Liu F, Song C, Yang D, Liu J (2014) Adenosine induces apoptosis in human liver cancer cells through ROS production and mitochondrial dysfunction. Biochem Biophys Res Commun 448(1):8–14. https://doi.org/10.1016/j.bbrc.2014.04.007 CrossRefPubMed

29.

Martinou JC, Youle RJ (2011) Mitochondria in apoptosis: Bcl-2 family members and mitochondrial dynamics. Dev Cell 21(1):92–101. https://doi.org/10.1016/j.devcel.2011.06.017 CrossRefPubMedPubMedCentral

30.

Kalashnikova I, Mazar J, Neal CJ, Rosado AL, Das S, Westmoreland TJ, Seal S (2017) Nanoparticle delivery of curcumin induces cellular hypoxia and ROS-mediated apoptosis via modulation of Bcl-2/Bax in human neuroblastoma. Nanoscale 9(29):10375–10387. https://doi.org/10.1039/c7nr02770b CrossRefPubMed

31.

Nebbioso A, Pereira R, Khanwalkar H, Matarese F, Garcia-Rodriguez J, Miceli M, Logie C, Kedinger V, Ferrara F, Stunnenberg HG, de Lera AR, Gronemeyer H, Altucci L (2011) Death receptor pathway activation and increase of ROS production by the triple epigenetic inhibitor UVI5008. Mol Cancer Ther 10(12):2394–2404. https://doi.org/10.1158/1535-7163.MCT-11-0525 CrossRefPubMed

32.

Xu C, Bailly-Maitre B, Reed JC (2005) Endoplasmic reticulum stress: cell life and death decisions. J Clin Invest 115(10):2656–2664. https://doi.org/10.1172/JCI26373 CrossRefPubMedPubMedCentral

33.

Teske BF, Fusakio ME, Zhou D, Shan J, McClintick JN, Kilberg MS, Wek RC (2013) CHOP induces activating transcription factor 5 (ATF5) to trigger apoptosis in response to perturbations in protein homeostasis. Mol Biol Cell 24:2477–2490. https://doi.org/10.1091/mbc.E13-01-0067 CrossRefPubMedPubMedCentral

34.

Li XF, Zhang Z, Chen ZK, Cui ZW, Zhang HN (2018) [Retracted] Piezo1 protein induces the apoptosis of human osteoarthritisderived chondrocytes by activating caspase12, the signaling marker of ER stress. Int J Mol Med 42(6):3640. https://doi.org/10.3892/ijmm.2018.3918 CrossRefPubMedPubMedCentral

35.

Luo Q, Yang D, Qi Q, Huang C, Chen B, Liu W, Shi L, Xia Y, Tang L, Fang J, Ou Y, Geng Y, Chen Z (2018) Role of the death receptor and endoplasmic reticulum stress signaling pathways in polyphyllin I-regulated apoptosis of human hepatocellular carcinoma HepG2 cells. Biomed Res Int 2018:5241941–5241911. https://doi.org/10.1155/2018/5241941 CrossRefPubMedPubMedCentral

36.

Sliman SM, Patel RB, Cruff JP, Kotha SR, Newland CA, Schrader CA, Sherwani SI, Gurney TO, Magalang UJ, Parinandi NL (2013) Adiponectin protects against hyperoxic lung injury and vascular leak. Cell Biochem Biophys 67(2):399–414. https://doi.org/10.1007/s12013-011-9330-1 CrossRefPubMedPubMedCentral

37.

Goldstein BJ, Scalia RG, Ma XL (2009) Protective vascular and myocardial effects of adiponectin. Nat Clin Pract Cardiovasc Med 6(1):27–35. https://doi.org/10.1038/ncpcardio1398 CrossRefPubMed

38.

Magalang UJ, Rajappan R, Hunter MG, Kutala VK, Kuppusamy P, Wewers MD, Marsh CB, Parinandi NL (2006) Adiponectin inhibits superoxide generation by human neutrophils. Antioxid Redox Signal 8(11–12):2179–2186. https://doi.org/10.1089/ars.2006.8.2179 CrossRefPubMed

39.

Potenza MA, Sgarra L, Nacci C, Leo V, De Salvia MA, Montagnani M (2019) Activation of AMPK/SIRT1 axis is required for adiponectin-mediated preconditioning on myocardial ischemia-reperfusion (I/R) injury in rats. PLoS One 14(1):e0210654. https://doi.org/10.1371/journal.pone.0210654 CrossRefPubMedPubMedCentral

40.

Chen Y, Qing W, Sun M, Lv L, Guo D, Jiang Y (2015) Melatonin protects hepatocytes against bile acid-induced mitochondrial oxidative stress via the AMPK-SIRT3-SOD2 pathway. Free Radic Res 49(10):1275–1284. https://doi.org/10.3109/10715762.2015.1067806 CrossRefPubMed

41.

Cheng Y, Dai C, Zhang J (2017) SIRT3-SOD2-ROS pathway is involved in linalool-induced glioma cell apoptotic death. Acta Biochim Pol 64(2):343–350. https://doi.org/10.18388/abp.2016_1438 CrossRefPubMed

Titel: Adiponectin ameliorates lung injury induced by intermittent hypoxia through inhibition of ROS-associated pulmonary cell apoptosis
verfasst von: Wenxiao Ding
Xilong Zhang
Qiang Zhang
Yanbin Dong
Wenjing Wang
Ning Ding
Publikationsdatum: 26.05.2020
Verlag: Springer International Publishing
Erschienen in: Sleep and Breathing / Ausgabe 1/2021
Print ISSN: 1520-9512
Elektronische ISSN: 1522-1709
DOI: https://doi.org/10.1007/s11325-020-02103-3

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Adiponectin ameliorates lung injury induced by intermittent hypoxia through inhibition of ROS-associated pulmonary cell apoptosis

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