01.12.2018 | Research | Ausgabe 1/2018 Open Access

Adipose stromal vascular fraction attenuates TH1 cell-mediated pathology in a model of multiple sclerosis
- Zeitschrift:
- Journal of Neuroinflammation > Ausgabe 1/2018
Background
Methods
EAE induction using myelin oligodendrocyte glycoprotein35–55 peptide
Isolation of the SVF cells and ASCs
Preparation and injection of cells
Tissue harvest and processing
Flow cytometric staining and analysis
RNA isolation from spleens
Transcript | Forward sequence (5’-3’) | Reverse sequence (5’-3’) |
---|---|---|
Interleukin-2 receptor β | GGCTCTTCTTGGAGATGCTG | GCCAGAAAAACAACCAAGGA |
Interleukin-23 | CAAAGGATCCGCCAAGGTCT | GGAGGTGTGAAGTTGCTCCA |
Foxp3 | TTGGCCAGCGCCATCTT | TGCCTCCTCCAGAGAGAAGTG |
STAT1 | CTTATTCCATGGA CAAGGTTTTG | GGTGCTTCTTAATGAGCTCTAGG |
T-bet | AACCAGTATCCTGTTCCCAGC | TGTCGCCACTGGAAGGATAG |
GATA-3 | CTCCTTTTTGCTCTCCTTTTC | AAGAGATGAGGACTGGAGTG |