The online version of this article (doi:10.1007/s00394-015-1087-7) contains supplementary material, which is available to authorized users.
Most of what we know about adipose tissue is restricted to observations derived after an overnight fast. However, humans spend the majority of waking hours in a postprandial (fed) state, and it is unclear whether increasing adiposity impacts adipose tissue responses to feeding. The aim of this research was to investigate postprandial responses in adipose tissue across varying degrees of adiposity.
Thirty males aged 35–55 years with waist circumference 81–118 cm were divided equally into groups categorized as either lean, overweight or obese. Participants consumed a meal and insulinaemic, glycaemic and lipidaemic responses were monitored over 6 h. Subcutaneous adipose tissue samples were obtained at baseline and after 6 h to examine changes in gene expression and adipose tissue secretion of various adipokines.
Following consumption of the meal, insulin and glucose responses were higher with increased adiposity (total AUC effects of group; p = 0.058 and p = 0.027, respectively). At 6 h, significant time effects reflected increases in IL-6 (F = 14.7, p = 0.001) and MCP-1 (F = 10.7, p = 0.003) and reduction in IRS2 adipose tissue gene expression (F = 24.6, p < 0.001), all independent of adiposity. Ex vivo adipokine secretion from adipose tissue explants remained largely unchanged after feeding.
Increased systemic measures of postprandial metabolism with greater adiposity do not translate into increased inflammatory responses within adipose tissue. Instead, postprandial adipose tissue changes may represent a normal response to feeding or a (relatively) normalized response with increased adiposity due to either similar net exposure (i.e. per g of adipose) or reduced adipose tissue responsiveness.
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Manning PJ, Sutherland WH, McGrath MM, de Jong SA, Walker RJ, Williams MJ (2008) Postprandial cytokine concentrations and meal composition in obese and lean women. Obesity (Silver Spring). doi: 10.1038/oby.2008.334
Meneses ME, Camargo A, Perez-Martinez P, Delgado-Lista J, Cruz-Teno C, Jimenez-Gomez Y, Paniagua JA, Gutierrez-Mariscal FM, Tinahones FJ, Vidal-Puig A, Roche HM, Perez-Jimenez F, Malagon MM, Lopez-Miranda J (2011) Postprandial inflammatory response in adipose tissue of patients with metabolic syndrome after the intake of different dietary models. Mol Nutr Food Res 55(12):1759–1770. doi: 10.1002/mnfr.201100200 CrossRef
World Health Organisation (2008) Waist circumference and waist–hip ratio: report of a WHO expert consultation, Geneva, 8–11 December
Travers RL, Motta AC, Betts JA, Bouloumie A, Thompson D (2014) The impact of adiposity on adipose tissue-resident lymphocyte activation in humans. Int J Obes (Lond). doi: 10.1038/ijo.2014.195
Hudgins LC, Hellerstein MK, Seidman CE, Neese RA, Tremaroli JD, Hirsch J (2000) Relationship between carbohydrate-induced hypertriglyceridemia and fatty acid synthesis in lean and obese subjects. J Lipid Res 41(4):595–604
Thompson D, Batterham AM, Bock S, Robson C, Stokes K (2006) Assessment of low-to-moderate intensity physical activity thermogenesis in young adults using synchronized heart rate and accelerometry with branched-equation modeling. The Journal of nutrition 136(4):1037–1042
Loney T, Standage M, Thompson D, Sebire SJ, Cumming S (2011) Self-report vs. objectively assessed physical activity: which is right for public health? J Phys Act Health 8(1):62–70 CrossRef
Paradisi G, Smith L, Burtner C, Leaming R, Garvey WT, Hook G, Johnson A, Cronin J, Steinberg HO, Baron AD (1999) Dual energy X-ray absorptiometry assessment of fat mass distribution and its association with the insulin resistance syndrome. Diabetes Care 22(8):1310–1317 CrossRef
Frayn KN (1983) Calculation of substrate oxidation rates in vivo from gaseous exchange. J Appl Physiol 55(2):628–634
Schofield WN (1985) Predicting basal metabolic rate, new standards and review of previous work. Hum Nutr Clin Nutr 39(Suppl 1):5–41
Betts JA, Thompson D, Richardson JD, Chowdhury EA, Jeans M, Holman GD, Tsintzas K (2011) Bath Breakfast Project (BBP)—examining the role of extended daily fasting in human energy balance and associated health outcomes: study protocol for a randomised controlled trial [ISRCTN31521726]. Trials 12:172. doi: 10.1186/1745-6215-12-172 CrossRef
Selimoglu H, Duran C, Kiyici S, Guclu M, Ersoy C, Ozkaya G, Erturk E, Tuncel E, Imamoglu S (2009) Comparison of composite whole body insulin sensitivity index derived from mixed meal test and oral glucose tolerance test in insulin resistant obese subjects. Endocrine 36(2):299–304. doi: 10.1007/s12020-009-9213-z CrossRef
Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985) Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28(7):412–419 CrossRef
Maxwell SE, Delaney HD (1990) Designing experiments and analysing data: a model comparison perspective. Wadsworth, Belmont
Atkinson G (2002) Analysis of repeated measurements in physical therapy research: multiple comparisons amongst level means and multi-factorial designs. Phys Ther Sport 3(4):191–203 CrossRef
Westerbacka J, Corner A, Kannisto K, Kolak M, Makkonen J, Korsheninnikova E, Nyman T, Hamsten A, Fisher RM, Yki-Jarvinen H (2006) Acute in vivo effects of insulin on gene expression in adipose tissue in insulin-resistant and insulin-sensitive subjects. Diabetologia 49(1):132–140. doi: 10.1007/s00125-005-0075-5 CrossRef
Pedersen DJ, Guilherme A, Danai LV, Heyda L, Matevossian A, Cohen J, Nicoloro SM, Straubhaar J, Noh LH, Jung DY, Kim JK, Czech MP (2015) A major role of insulin in promoting obesity-associated adipose tissue inflammation. Mol Metab 4(7):507–518 CrossRef
Meugnier E, Faraj M, Rome S, Beauregard G, Michaut A, Pelloux V, Chiasson JL, Laville M, Clement K, Vidal H, Rabasa-Lhoret R (2007) Acute hyperglycemia induces a global downregulation of gene expression in adipose tissue and skeletal muscle of healthy subjects. Diabetes 56(4):992–999. doi: 10.2337/db06-1242 CrossRef
Moro C, Klimcakova E, Lolmede K, Berlan M, Lafontan M, Stich V, Bouloumie A, Galitzky J, Arner P, Langin D (2007) Atrial natriuretic peptide inhibits the production of adipokines and cytokines linked to inflammation and insulin resistance in human subcutaneous adipose tissue. Diabetologia 50(5):1038–1047. doi: 10.1007/s00125-007-0614-3 CrossRef
Curat CA, Miranville A, Sengenes C, Diehl M, Tonus C, Busse R, Bouloumie A (2004) From blood monocytes to adipose tissue-resident macrophages: induction of diapedesis by human mature adipocytes. Diabetes 53(5):1285–1292 CrossRef
Zeyda M, Farmer D, Todoric J, Aszmann O, Speiser M, Gyori G, Zlabinger GJ, Stulnig TM (2007) Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production. Int J Obes (Lond) 31(9):1420–1428. doi: 10.1038/sj.ijo.0803632 CrossRef
Freemerman AJ, Johnson AR, Sacks GN, Milner JJ, Kirk EL, Troester MA, Macintyre AN, Goraksha-Hicks P, Rathmell JC, Makowski L (2014) Metabolic reprogramming of macrophages: glucose transporter 1 (GLUT1)-mediated glucose metabolism drives a proinflammatory phenotype. J Biol Chem 289(11):7884–7896. doi: 10.1074/jbc.M113.522037 CrossRef
Sampey BP, Vanhoose AM, Winfield HM, Freemerman AJ, Muehlbauer MJ, Fueger PT, Newgard CB, Makowski L (2011) Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet. Obesity (Silver Spring) 19(6):1109–1117. doi: 10.1038/oby.2011.18 CrossRef
Bastard JP, Jardel C, Bruckert E, Blondy P, Capeau J, Laville M, Vidal H, Hainque B (2000) Elevated levels of interleukin 6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss. J Clin Endocrinol Metab 85(9):3338–3342. doi: 10.1210/jcem.85.9.6839
Scheller J, Chalaris A, Schmidt-Arras D (1813) Rose-John S (2011) The pro- and anti-inflammatory properties of the cytokine interleukin-6. Biochim Biophys Acta 5:878–888. doi: 10.1016/j.bbamcr.2011.01.034
- Adipose tissue metabolic and inflammatory responses to a mixed meal in lean, overweight and obese men
Rebecca L. Travers
Alexandre C. Motta
James A. Betts
- Springer Berlin Heidelberg