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05.05.2016 | Original Contribution

Agaricus brasiliensis (sun mushroom) affects the expression of genes related to cholesterol homeostasis

verfasst von: Aline Mayrink de Miranda, Joamyr Victor Rossoni Júnior, Lorena Souza e Silva, Rinaldo Cardoso dos Santos, Marcelo Eustáquio Silva, Maria Lúcia Pedrosa

Erschienen in: European Journal of Nutrition | Ausgabe 4/2017

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Abstract

Purpose

The sun mushroom (Agaricus brasiliensis) is considered a major source of bioactive compounds with potential health benefits. Mushrooms typically act as lipid-lowering agents; however, little is known about the mechanisms of action of A. brasiliensis in biological systems. This study aimed to determine the underlying mechanism involved in the cholesterol-lowering effect of A. brasiliensis through the assessment of fecal and serum lipid profiles in addition to gene expression analysis of specific transcription factors, enzymes, and transporters involved in cholesterol homeostasis.

Methods

Twenty-four albino Fischer rats approximately 90 days old, with an average weight of 205 g, were divided into four groups of 6 each and fed a standard AIN-93 M diet (C), hypercholesterolemic diet (H), hypercholesterolemic diet +1 % A. brasiliensis (HAb), or hypercholesterolemic diet +0.008 % simvastatin (HS) for 6 weeks. Simvastatin was used as a positive control, as it is a typical drug prescribed for lipid disorders. Subsequently, blood, liver, and feces samples were collected for lipid profile and quantitative real-time polymerase chain reaction gene expression analyses.

Results

Diet supplementation with A. brasiliensis significantly improved serum lipid profiles, comparable to the effect observed for simvastatin. In addition, A. brasiliensis dietary supplementation markedly promoted fecal cholesterol excretion. Increased expression of 7α-hydroxylase (CYP7A1), ATP-binding cassette subfamily G-transporters (ABCG5/G8), and low-density lipoprotein receptor (LDLR) was observed following A. brasiliensis administration.

Conclusions

Our results suggest that consumption of A. brasiliensis improves the serum lipid profile in hypercholesterolemic rats by modulating the expression of key genes involved in hepatic cholesterol metabolism.

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Titel: Agaricus brasiliensis (sun mushroom) affects the expression of genes related to cholesterol homeostasis
verfasst von: Aline Mayrink de Miranda
Joamyr Victor Rossoni Júnior
Lorena Souza e Silva
Rinaldo Cardoso dos Santos
Marcelo Eustáquio Silva
Maria Lúcia Pedrosa
Publikationsdatum: 05.05.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 4/2017
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-016-1217-x

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Agaricus brasiliensis (sun mushroom) affects the expression of genes related to cholesterol homeostasis

Abstract

Purpose

Methods

Results

Conclusions

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