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13.02.2019 | Original Research Paper | Ausgabe 4/2019

Inflammation Research 4/2019

AIM2 levels and DNA-triggered inflammasome response are increased in peripheral leukocytes of patients with abdominal aortic aneurysm

Zeitschrift:
Inflammation Research > Ausgabe 4/2019
Autoren:
Markus Wortmann, Xianghui Xiao, Guido Wabnitz, Yvonne Samstag, Maani Hakimi, Dittmar Böckler, Susanne Dihlmann
Wichtige Hinweise
Responsible Editor: Andrew Roberts.

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1007/​s00011-019-01212-4) contains supplementary material, which is available to authorized users.
This study was presented at the Annual meeting of the German Society for Vascular Surgery and Vascular Medicine in Frankfurt, Germany, September, 27–30, 2017.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Abstract

Objective and design

Abdominal aortic aneurysm (AAA) is heavily infiltrated with leukocytes, expressing the DNA sensor absent in melanoma 2 (AIM2) and other inflammasome components.

Methods

Using multicolour flow cytometry, we here compared the expression of the inflammasome components AIM2, NLRP3, and ASC in different peripheral immune cells derived from AAA patients with those from non-AAA patients in a case–control study. In parallel, peripheral blood mononuclear cells (PBMC) of AAA patients and controls were stimulated in vitro with poly-dA:dT or lipopolysaccharide (LPS) to analyze inflammasome activation.

Results

AIM2 expression was significantly increased in peripheral granulocytes (P = 0.026), monocytes (P = 0.007), B lymphocytes (P < 0.0001), and T lymphocytes (P = 0.004) of AAA patients. Expression of other inflammasome components did not differ between the groups. Following in vitro stimulation with foreign DNA, PBMC derived from AAA patients released significantly more IL-1β (P = 0.022) into the supernatant than PBMC from control patients. In contrast, IL-1β release upon LPS stimulation did not differ between the PBMC groups.

Conclusion

The data indicate the increased activation of an AIM2 inflammasome in peripheral immune cells of AAA patients and point to a systemic AIM2-associated immune response to AAA.

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