The online version of this article (doi:10.1186/s13045-015-0125-5) contains supplementary material, which is available to authorized users.
Sichu Liu and Qi Shen contributed equally to this work.
The authors declare that they have no competing interests.
YQL contributed to concept development and study design. SCL and QS performed the cell culture, nucleofection, and RNA isolation and data analysis. YC, CWZ, CSC, XLW and BL helped to array data analysis, LJY and SHC helped to cell culture and collect samples. YQL and SCL coordinated the study and helped draft the manuscript. All authors read and approved the final manuscript.
We reported that knockdown of PPP2R5C by siRNA led to proliferation inhibition and apoptosis induction in K562 cells. In this study, we further characterized the gene expression profiles after PPP2R5C suppression by microarray analysis. Genes which participate in the MAPK, PI3K/AKT, and JAK/STAT pathways, were mainly altered in the K562 cells. We propose that the mechanism for proliferation inhibition and increased apoptosis of K562 cells following PPP2R5C suppression may be related to the alteration of expression profiles of BRAF, AKT2, AKT3, NFKB2 and STAT3 genes.
Additional file 1: Methods and materials.13045_2015_125_MOESM1_ESM.docx
- Alteration of gene expression profile following PPP2R5C knockdown may be associated with proliferation suppression and increased apoptosis of K562 cells
- BioMed Central
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