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Journal of Assisted Reproduction and Genetics

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06.04.2016 | Gamete Biology

Alteration of spermatogenesis following spermatogonial stem cells transplantation in testicular torsion-detorsion mice

verfasst von: Saeid Azizollahi, Reza Aflatoonian, Mohammad Ali Sadighi Gilani, Babak Behnam, Nader Tajik, Mohammad Asghari-Jafarabadi, Hamid Reza Asgari, Morteza Koruji

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 6/2016

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Abstract

Purpose

Testicular ischemia is the main consequence of testicular torsion, in both clinical and experimental aspects. Preservation and auto-transplantation of spermatogonial stem cells (SSCs) could be a new treatment for infertility in testicular ischemia following testicular torsion.

Methods

To apply the idea in this study, animals were randomly divided into four groups of control, sham, with torsion, and with torsion followed by transplantation (TT). Isolated SSCs from neonatal mice were cultured and identified by flow cytometry (C-KIT⁻, INTEGRIN β₁ ⁺) and RT-PCR (Reverse transcription polymerase chain reaction) for specific spermatogonial cell markers (Oct4, Gfrα-1, Plzf, Vasa, Itgα ₆, and Itgβ ₁). SSCs were transplanted upon a 2-h testicular torsion in the TT group. Cultured cells were transplanted into ischemia reperfusion testicle 2 weeks post-testicular torsion. Eight weeks after SSCs transplantation, the SSCs-transplanted testes and epididymis were removed for sperm analysis, weight and histopathological evaluation, and pre- and post-meiotic gene expression assessment by qRT-PCR.

Results

Our findings indicated that all evaluated parameters (epididymal sperm profile, Johnsen score, Plzf, Gfrα-1, Scp-1, Tekt-1 expressions, and histopathological profile) were significantly decreased following testicular torsion (group 3) when compared to the control group (p ≤ 0.05). However, all abovementioned parameters showed a significant increase/improvement in torsion-transplantation group compared to torsion group. However, these parameters in the TT group were significantly lower in the sham and control groups (p ≤ 0.05).

Conclusion

SSCs transplantation could up-regulate the expression of pre- and post-meiotic genes in testicular ischemia, which resulted in improvement of both testicular function and structure after testicular torsion.

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Titel: Alteration of spermatogenesis following spermatogonial stem cells transplantation in testicular torsion-detorsion mice
verfasst von: Saeid Azizollahi
Reza Aflatoonian
Mohammad Ali Sadighi Gilani
Babak Behnam
Nader Tajik
Mohammad Asghari-Jafarabadi
Hamid Reza Asgari
Morteza Koruji
Publikationsdatum: 06.04.2016
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 6/2016
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0708-2

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Alteration of spermatogenesis following spermatogonial stem cells transplantation in testicular torsion-detorsion mice

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

Purpose

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