Erschienen in:
17.05.2016 | ORIGINAL ARTICLE
Altered Cyclooxygenase-2 Expression in Pulmonary Sarcoidosis is not Related to Clinical Classifications
verfasst von:
Justyna Kiszałkiewicz, Wojciech J. Piotrowski, Dorota Pastuszak-Lewandoska, Paweł Górski, Adam Antczak, Witold Górski, Daria Domańska-Senderowska, Monika Migdalska-Sęk, Karolina H. Czarnecka, Ewa Nawrot, Ewa Brzeziańska-Lasota
Erschienen in:
Inflammation
|
Ausgabe 4/2016
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Abstract
Elevated COX-2 activity is associated with the development of chronic lung diseases leading to bronchial obstruction, including sarcoidosis. The aim of the study was to examine expression pattern of COX-2 messenger RNA (mRNA). Expression was performed by q-PCR method in bronchoalveolar lavage (BAL) cells and peripheral blood (PB) lymphocytes in sarcoidosis patients (n = 61) and control group (n = 30). Analysis of COX-2 mRNA expression level in BAL fluid and PB revealed downregulation in sarcoidosis and control groups. In PB lymphocytes, the statistically significant difference between patients and controls was observed (P = 0.003, Mann–Whitney U test), with higher expression in patients. There were no statistically significant differences between patients without and with parenchymal involvement (stages I vs. II–IV), between patients with acute vs. insidious onset of disease and between patients with abnormal vs. normal spirometry (P > 0.05, Mann–Whitney U test). Results suggest that expression of COX-2 mRNA in patients with pulmonary sarcoidosis is not related to clinical classifications.