Skip to main content
main-content

15.12.2016 | Original Article | Ausgabe 3/2017

Virchows Archiv 3/2017

Altered expression of apoptosis-regulating miRNAs in salivary gland tumors suggests their involvement in salivary gland tumorigenesis

Zeitschrift:
Virchows Archiv > Ausgabe 3/2017
Autoren:
Bianca de Cássia Troncarelli de Campos Parra Flores, Silvia Vanessa Lourenço, Aline Santos Damascena, Luiz Paulo Kowaslki, Fernando Augusto Soares, Cláudia Malheiros Coutinho-Camillo
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s00428-016-2049-z) contains supplementary material, which is available to authorized users.

Abstract

Salivary gland tumors comprise a heterogeneous group of lesions with different histological features and diverse clinical pathophysiology. They account for about 3% of all head and neck tumors. Apoptosis plays an important role during morphogenesis of glandular structures, including that of the salivary gland. Recent studies have demonstrated that several microRNAs (miRNAs) are involved in the control of apoptosis. The aim of the present study was to determine the expression of apoptosis-related miRNAs (miR-15a, miR-16, miR-17-5p, miR-20a, miR-21, miR-29, and miR-34) and their target mRNAs in 25 pleomorphic adenomas, 23 mucoepidermoid carcinomas, and 10 non-neoplastic salivary gland samples by real-time RT-PCR. We observed upregulation of miR-15a, miR-16, miR-17-5p, miR-21, miR-29, and miR-34a in pleomorphic adenomas. The expression of miR-21 and miR-34a was upregulated in 91 and 74% of mucoepidermoid carcinomas, respectively. Downregulation of miR-20a was observed in 75% of pleomorphic adenomas and in 57% of mucoepidermoid carcinomas. APAF1, BAX, BCL2, BID, CASP2, CASP8, DIABLO , and TP53 transcripts were upregulated in both tumor types. BAD transcripts were upregulated in pleomorphic adenomas. CASP3 and CASP6 transcripts were upregulated in mucoepidermoid carcinomas. BCL2, CASP2, CASP6, and CASP8 proteins were mostly absent in mucoepidermoid carcinomas but expressed in few cells in pleomorphic adenomas. Our study provides evidence of alterations in the expression of apoptosis-regulating miRNAs in salivary gland tumors, suggesting possible involvement of these microRNAs in salivary gland tumorigenesis.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Premium-Inhalten der Fachzeitschriften, inklusive eines Print-Abos.

Zusatzmaterial
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 3/2017

Virchows Archiv 3/2017Zur Ausgabe

Neu im Fachgebiet Pathologie

26.06.2018 | Prostatakarzinom | CME | Ausgabe 4/2018

CME: Neuroendokrines Prostatakarzinom

19.06.2018 | Schwerpunkt: Pathologie und Forschungsbiobanken | Ausgabe 4/2018

Der Aufbau und Betrieb einer Zentralen Biomaterialbank

Die ZeBanC der Charité Berlin

06.06.2018 | Schwerpunkt: Pathologie und Forschungsbiobanken | Ausgabe 4/2018

Biobanking und die Weiterentwicklung der Präzisionsmedizin

23.05.2018 | Schwerpunkt: Pathologie und Forschungsbiobanken | Ausgabe 4/2018

Alle unter einem Dach

Erfolge und Herausforderungen auf dem Weg zu einer zentralisierten Biobank am Beispiel der BMBH