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01.03.2012 | Original Paper

Altered mRNA expressions of sialyltransferases in human gastric cancer tissues

verfasst von: Li Jun, Wang Yuanshu, Xie Yanying, Xu Zhongfa, Yang Jian, Wang Fengling, Qu Xianjun, Norihiro Kokudo, Tang Wei, Zhong Weixia, Cui Shuxiang

Erschienen in: Medical Oncology | Ausgabe 1/2012

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Abstract

Aberrant sialylation and altered sialyltransferase (ST) expressions are frequently found in many types of cancer. In this study, we examined the expressions of α 2,3-linked sialic acid residues and STs mRNA in human gastric cancer tissues. The relationship between the levels of α 2,3-linked sialic acid residues and the expression of STs mRNA was also analyzed. Maackia amurensis leukoagglutinin (MAL) specific for α 2,3-linked sialic acid was used for immunohistochemical staining. All gastric cancer tissues were positive for MAL staining, whereas adjacent normal tissues were negative. The extent of MAL staining was significantly correlated with the behavior of gastric cancer. The expression of STs mRNA was examined by real-time quantitative reverse transcription-polymerase chain reaction assay. The levels of ST3Gal IV and ST6GalI in gastric cancer tissues were significantly increased compared with those in adjacent normal tissues (P < 0.05), whereas the levels of ST3Gal I, ST3Gal III, and ST3Gal VI were not increased. Furthermore, the high expression of ST3Gal IV was closely related to the extent of MAL staining (P < 0.05) unlike that of ST6Gal I. These results indicated that gastric cancer tissues expressed high levels of α 2,3-linked sialic acid residues, ST3Gal IV, and ST6Gal I. The high expression of ST3Gal IV may contribute to the expression of α 2,3-linked sialic acid residues, which is associated with the malignant behavior of gastric cancer cells.

Sawada M, Moriya S, Saito S, Shineha R, Satomi S, Yamori T, Tsuruo T, Kannagi R, Miyagi T. Reduced sialidase expression in highly metastatic variants of mouse colon adenocarcinoma 26 and retardation of their metastatic ability by sialidase overexpression. Int J Cancer. 2002;97:180–5.PubMedCrossRef

Lin S, Kemmner W, Grigull S, Schlag PM. Cell surface alpha 2,6 sialylation affects adhesion of breast carcinoma cells. Exp Cell Res. 2002;276:101–10.PubMedCrossRef

Babal P, Janega P, Cerna A, Kholova I, Brabencova E. Neoplastic transformation of the thyroid gland is accompanied by changes in cellular sialylation. Acta Histochem. 2006;108:133–40.PubMedCrossRef

Wang WC, Cummings RD. The immobilized leukoagglutinin from the seeds of Maackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked α-2, 3 to penultimate galactose residues. J Biol Chem. 1988;263:4576–85.PubMed

Tang W, Mafune K, Nakata M, Konishi T, Kojima N, Mizuochi T, Makuuchi M. Association of histochemical expression of Maackia amurensis leukoagglutinin-positive glycoconjugates with behavior of human gastric cancer. Histopathology. 2003;42:239–45.PubMedCrossRef

Wang FL, Cui SX, Sun LP, Qu XJ, Xie YY, Zhou L, Mu YL, Tang W, Wang YS. High expression of alpha 2, 3-linked sialic acid residues is associated with the metastatic potential of human gastric cancer. Cancer Detect Prev. 2009;32:437–43.PubMedCrossRef

Yamashita K, Sakuramoto S, Kikuchi S, Katada N, Kobayashi N, Watanabe M. Validation of staging systems for gastric cancer. Gastric Cancer. 2008;11:111–8.PubMedCrossRef

Li C, et al. Macroscopic Borrmann type as a simple prognostic indicator in patients with advanced gastric cancer. Oncology. 2009;77(3–4):197–204.PubMedCrossRef

Czyzewska J, Guzinska-Ustymowicz K, Kemona A, Bandurski R. The expression of matrix metalloproteinase 9 and cathepsin B in gastric carcinoma is associated with lymph node metastasis, but not with postoperative survival. Folia Histochem Cytobiol. 2008;46:57–64.PubMedCrossRef

10.

Julian J, Carson DD. Formation of MUC1 metabolic complex is conserved in tumor-derived and normal epithelial cells. Biochem Biophys Res Commun. 2002;293:1183–90.PubMedCrossRef

11.

Wang PH, Lee WL, Juang CM, Yang YH, Lo WH, Lai CR, Hsieh SL, Yuan CC. Altered mRNA expressions of sialyltransferases in ovarian cancers. Gynecol Oncol. 2005;99:631–9.PubMedCrossRef

12.

Pengju Z, Weiwen C, Aiying W, Zhaobo C, Nana N, Zhaoqin H, Qingwei L, Anli J. NKX3.1 potentiates TNF-alpha/CHX-induced apoptosis of prostate cancer cells through increasing caspase-3 expression and its activity. Biochem Biophys Res Commun. 2010;398:457–61.PubMedCrossRef

13.

Kannagi R. Carbohydrate-mediated cell adhesion involved in hematogenous metastasis of cancer. Glycoconj J. 1997;14:577–84.PubMedCrossRef

14.

Varki A. Sialic acids in human health and disease. Trends Mol Med. 2008;14:351–60.PubMedCrossRef

15.

Hakomori S. Glycosylation defining cancer malignancy: new wine in an old bottle. Proc Natl Acad Sci USA. 2002;99:10231–3.PubMedCrossRef

16.

Ohyama C, Hosono M, Nitta K, Oh-eda M, Yoshikawa K, Habuchi T, Arai Y, Fukuda M. Carbohydrate structure and differential binding of prostate specific antigen to Maackia amurensis lectin between prostate cancer and benign prostate hypertrophy. Glycobiology. 2004;14:671–9.PubMedCrossRef

17.

Kannagi R. Carbohydrate antigen sialyl Lewis a–its pathophysiological significance and induction mechanism in cancer progression. Chang Gung Med J. 2007;30:189–209.PubMed

18.

Varki A. Glycan-based interactions involving vertebrate sialic-acid-recognizing proteins. Nature. 2007;446:1023–9.PubMedCrossRef

19.

Christie DR, Shaikh FM, Lucas JA IV, Lucas JA III, Bellis SL. ST6Gal-I expression in ovarian cancer cells promotes an invasive phenotype by altering integrin glycosylation and function. J Ovarian Res. 2008;1:3.PubMedCrossRef

20.

Seales EC, Jurado GA, Brunson BA, Wakefield JK, Frost AR, Bellis SL. Hypersialylation of beta1 integrins, observed in colon adenocarcinoma, may contribute to cancer progression by up-regulating cell motility. Cancer Res. 2005;65:4645–52.PubMedCrossRef

21.

Jain RK, Piskorz CF, Huang BG, Locke RD, Han HL, Koenig A, Varki A, Matta KL. Inhibition of L- and P-selectin by a rationally synthesized novel core 2-like branched structure containing GalNAc-Lewisx and Neu5Acalpha2-3Galbeta1-3GalNAc sequences. Glycobiology. 1998;8:707–17.PubMedCrossRef

22.

Kannagi R, Izawa M, Koike T, Miyazaki K, Kimura N. Carbohydrate-mediated cell adhesion in cancer metastasis and angiogenesis. Cancer Sci. 2004;95:377–84.PubMedCrossRef

23.

Takada A, Ohmori K, Yoneda T, Tsuyuoka K, Hasegawa A, Kiso M, Kannagi R. Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium. Cancer Res. 1993;53:354–61.PubMed

24.

Lopez-Morales D, Velazquez-Marquez N, Valenzuela O, Santos-Lopez G, Reyes-Leyva J, Vallejo-Ruiz V. Enhanced sialyltransferases transcription in cervical intraepithelial neoplasia. Invest Clin. 2009;50:45–53.PubMed

25.

Damiani R, Oliveira JE, Vorauer-Uhl K, Peroni CN, Vianna EG, Bartolini P, Ribela MT. Stable expression of a human-like sialylated recombinant thyrotropin in a Chinese hamster ovary cell line expressing alpha 2,6-sialyltransferase. Protein Expr Purif. 2009;67:7–14.PubMedCrossRef

26.

Taniguchi A. Promoter structure and transcriptional regulation of human beta-galactoside alpha 2, 3-sialyltransferase genes. Curr Drug Targets. 2008;9:310–6.PubMedCrossRef

27.

Petretti T, Schulze B, Schlag PM, Kemmner W. Altered mRNA expression of glycosyltransferases in human gastric carcinomas. Biochim Biophys Acta. 1999;1428:209–18.PubMedCrossRef

28.

Petretti T, Kemmner W, Schulze B, Schlag PM. Altered mRNA expression of glycosyltransferases in human colorectal carcinomas and liver metastases. Gut. 2000;46:359–66.PubMedCrossRef

Titel: Altered mRNA expressions of sialyltransferases in human gastric cancer tissues
verfasst von: Li Jun
Wang Yuanshu
Xie Yanying
Xu Zhongfa
Yang Jian
Wang Fengling
Qu Xianjun
Norihiro Kokudo
Tang Wei
Zhong Weixia
Cui Shuxiang
Publikationsdatum: 01.03.2012
Verlag: Springer US
Erschienen in: Medical Oncology / Ausgabe 1/2012
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-010-9771-1

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Altered mRNA expressions of sialyltransferases in human gastric cancer tissues

Abstract

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Abstract

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