Stroke is a condition characterized by damage to the cerebral vasculature from various causes, resulting in focal or widespread brain tissue damage. Prior neuroimaging research has demonstrated that individuals with stroke present structural and functional brain abnormalities, evident through disruptions in motor, cognitive, and other vital functions. Nevertheless, there is a lack of studies on alterations in static and dynamic functional network connectivity in the brains of stroke patients. Fifty stroke patients and 50 healthy controls (HCs) underwent resting-state functional magnetic resonance imaging (rs-fMRI) scanning. Initially, the independent component analysis (ICA) method was utilized to extract the resting-state network (RSN). Subsequently, the disparities in static functional network connectivity both within and between networks among the two groups were computed and juxtaposed. Following this, five consistent and robust dynamic functional network connectivity (dFNC) states were derived by integrating the sliding time window method with k-means cluster analysis, and the distinctions in dFNC between the groups across different states, along with the intergroup variations in three dynamic temporal metrics, were assessed. Finally, a support vector machine (SVM) approach was employed to discriminate stroke patients from HCs using FC and FNC as classification features. Comparing the stroke group to the healthy control (HC) group, the stroke group exhibited reduced intra-network functional connectivity (FC) in the right superior temporal gyrus of the ventral attention network (VAN), the left calcarine of the visual network (VN), and the left precuneus of the default mode network (DMN). Regarding static functional network connectivity (FNC), we identified increased connectivity between the executive control network (ECN) and dorsal attention network (DAN), salience network (SN) and DMN, SN-ECN, and VN-ECN, along with decreased connectivity between DAN-DAN, ECN-SN, SN-SN, and DAN-VN between the two groups. Noteworthy differences in dynamic FNC (dFNC) were observed between the groups in states 3 to 5. Moreover, stroke patients demonstrated a significantly higher proportion of time and longer mean dwell time in state 4, alongside a decreased proportion of time in state 5 compared to HC. Finally, utilizing FC and FNC as features, stroke patients could be distinguished from HC with an accuracy exceeding 70% and an area under the curve ranging from 0.8284 to 0.9364. In conclusion, our study reveals static and dynamic changes in large-scale brain networks in stroke patients, potentially linked to abnormalities in visual, cognitive, and motor functions. This investigation offers valuable insights into the neural mechanisms underpinning the functional deficits observed in stroke, thereby aiding in the diagnosis and development of targeted therapeutic interventions for affected individuals.
