AM22, a novel synthetic microRNA, inhibits the proliferation of colorectal cancer cells by targeting core binding factor subunit β (CBFB)
- 05.01.2022
- PRECLINICAL STUDIES
Erschienen in:
- Verfasst von
- Fanyi Meng
- Jiawei Li
- Yajing Qiu
- Haiyang Zhang
- Hongjian Zhang
- Weipeng Wang
- Erschienen in
- Investigational New Drugs | Ausgabe 3/2022
Summary
Our previous studies have revealed the important roles of the nonseed regions of microRNAs (miRNAs) in gene regulation, which provided novel insight into the development of miRNA analogs for cancer therapy. Here, we altered each nucleotide in the nonseed region of miR-34a and obtained novel synthetic miRNA analogs. Among them, AM22, with a base alteration from G to C at the 17th nucleotide of miR-34a, showed extensive antiproliferative activity against several colorectal tumor cell lines and achieved effective inhibition of core binding factor subunit β (CBFB) expression. Subsequent investigations demonstrated that AM22 directly targeted CBFB by binding to its 3'-untranslated region (3'-UTR). Inhibition of CBFB showed obvious antiproliferative activity on HCT-116 and SW620 cells. Furthermore, the antiproliferative effects of AM22 on these cells were also measured in xenograft mouse models. In conclusion, this study identified AM22 as a potential antitumor miRNA by targeting CBFB and provided a new design approach for miRNA-based cancer treatment by changing the nonseed region of miRNA.
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- Titel
- AM22, a novel synthetic microRNA, inhibits the proliferation of colorectal cancer cells by targeting core binding factor subunit β (CBFB)
- Verfasst von
-
Fanyi Meng
Jiawei Li
Yajing Qiu
Haiyang Zhang
Hongjian Zhang
Weipeng Wang
- Publikationsdatum
- 05.01.2022
- Verlag
- Springer US
- Erschienen in
-
Investigational New Drugs / Ausgabe 3/2022
Print ISSN: 0167-6997
Elektronische ISSN: 1573-0646 - DOI
- https://doi.org/10.1007/s10637-021-01208-0
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