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18.02.2017 | Case Report

Amplification of blood smear DNA to confirm disseminated histoplasmosis

verfasst von: T. Dieng, A. Massaly, D. Sow, S. Vellaissamy, K. Sylla, R. C. Tine, Y. Dieng, C. Hennequin

Erschienen in: Infection | Ausgabe 5/2017

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Abstract

Background

The prevalence of the Histoplasma capsulatum var. capsulatum (Hcc) histoplasmosis may be underestimated West Africa, both because the diagnosis is not mentioned in the early stages of the disease and due to limited biological resources available.

Case Report

We report a case of disseminated histoplasmosis due to Hcc in a Senegalese HIV patient. The diagnosis was suspected following the demonstration of small encapsulated yeasts within neutrophils on a thin blood smear. It was further confirmed using a specific real-time PCR applied on a DNA specimen extracted from the thin blood smear.

Conclusion

To the best of our knowledge, this is the first case of Hcc infection diagnosed in Senegal. Blood smear may be a valuable screening tool in the case of bloodstream dissemination and can be used for further molecular approaches to confirm the diagnosis.

Wheat LJ, Azar MM, Bahr NC, Spec A, Relich RF, Hage C. Histoplasmosis. Infect Dis Clin North Am. 2016;30:207–27. doi:10.1016/j.idc.2015.10.009.CrossRefPubMed

Gugnani HC. Histoplasmosis in Africa: a review. Indian J Chest Dis Allied Sci. 2000;42:271–7.PubMed

Buitrago MJ, Berenguer J, Mellado E, Rodriguez-Tudela JL, Cuenca-Estrella M. Detection of imported histoplasmosis in serum of HIV-infected patients using a real-time PCR-based assay. Eur J Clin Microbiol Infect Dis. 2006;25:665–8. doi:10.1007/s10096-006-0207-y.CrossRefPubMed

Ndiaye D, Diallo M, Sene PD, Ndiaye M, Ndir O. Disseminated histoplasmosis due to Histoplasma capsulatum var. duboisii in Senegal. A case in HIV-infected patient. J Mycol Med. 2011;21:60–4. doi:10.1016/j.mycmed.2010.12.004.CrossRefPubMed

Diadie S, Diatta B, Ndiaye M, Gaye M, Sow D, Ndiaye MT, et al. Multifocal histoplasmosis due to Histoplasma capsulatum var. duboisii in a 22 year-old Senegalese patient without proven immunodepression. J Mycol Med. 2016;. doi:10.1016/j.mycmed.2016.03.004.

Kourda N, Mlika M, Zidi YS, Zermani R, Ben Jilani S. Cecal perforation in a kidney transplant patient: disseminated histoplasmosis. Med Trop (Mars). 2010;70:533–6.

Debat Zoguereh D, Bigel ML, Billy C, Perronne V, Richardin F, Granier F. Disseminated histoplasmosis revealed by peripheral blood smear in an African immigrant with AIDS. Med Mal Infect. 2008;38:228–30. doi:10.1016/j.medmal.2007.10.009.CrossRefPubMed

Inojosa W, Rossi MC, Laurino L, Giobbia M, Fuser R, Carniato A, et al. Progressive disseminated histoplasmosis among human immunodeficiency virus-infected patients from West-Africa: report of four imported cases in Italy. Infez Med. 2011;19:49–55.PubMed

Mandengue CE, Ngandjio A, Atangana PJ. Histoplasmosis in HIV-infected persons, Yaounde, Cameroon. Emerg Infect Dis. 2015;21:2094–6. doi:10.3201/eid2111.150278.CrossRefPubMedPubMedCentral

10.

Rivasi F, Casali B, Nanetti A, Collina G, Mazzoni A. Histoplasma capsulatum var. capsulatum occurring in an HIV-positive Ghanaian immigrant to Italy. Identification of H. capsulatum DNA by PCR from paraffin sample. APMIS. 2001;109:721–5.CrossRefPubMed

11.

Manzini M, Lavinsky-Wolff M. Nasal histoplasmosis without lung involvement in an immunocompromised patient. Braz J Otorhinolaryngol. 2012;78:136.CrossRefPubMed

12.

Ubesie AC, Okafo OC, Ibeziako NS, Onukwuli VO, Mbanefo NR, Uzoigwe JC, et al. Disseminated histoplasmosis in a 13-year-old girl: a case report. Afr Health Sci. 2013;13:518–21. doi:10.4314/ahs.v13i2.45.PubMedPubMedCentral

13.

Enache-Angoulvant A, Torti F, Tassart M, Poirot JL, Jafari A, Roux P, et al. Candidal abscess of the parotid gland due to Candida glabrata: report of a case and literature review. Med Mycol. 2010;48:402–5. doi:10.3109/13693780903176503.CrossRefPubMed

14.

Iriart X, Blanchet D, Menard S, Lavergne RA, Chauvin P, Adenis A, et al. A complementary tool for management of disseminated Histoplasma capsulatum var. capsulatum infections in AIDS patients. Int J Med Microbiol. 2014;304:1062–5. doi:10.1016/j.ijmm.2014.07.016.CrossRefPubMed

15.

Ranque S, Pelletier R, Michel-Nguyen A, Dromer F. Platelia Aspergillus assay for diagnosis of disseminated histoplasmosis. Eur J Clin Microbiol Infect Dis. 2007;26:941–3. doi:10.1007/s10096-007-0380-7.CrossRefPubMed

16.

Babady NE, Buckwalter SP, Hall L, Le Febre KM, Binnicker MJ, Wengenack NL. Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR. J Clin Microbiol. 2011;49:3204–8. doi:10.1128/JCM.00673-11.CrossRefPubMedPubMedCentral

17.

Simon S, Veron V, Boukhari R, Blanchet D, Aznar C. Detection of Histoplasma capsulatum DNA in human samples by real-time polymerase chain reaction. Diagn Microbiol Infect Dis. 2010;66:268–73. doi:10.1016/j.diagmicrobio.2009.10.010.CrossRefPubMed

18.

Cnops L, Van Esbroeck M, Bottieau E, Jacobs J. Giemsa-stained thick blood films as a source of DNA for Plasmodium species-specific real-time PCR. Malar J. 2010;9:370. doi:10.1186/1475-2875-9-370.CrossRefPubMedPubMedCentral

19.

Polley SD, Boadi S, Watson J, Curry A, Chiodini PL. Detection and species identification of microsporidial infections using SYBR Green real-time PCR. J Med Microbiol. 2011;60:459–66. doi:10.1099/jmm.0.026781-0.CrossRefPubMed

20.

Teixeira Mde M, Patane JS, Taylor ML, Gomez BL, Theodoro RC, de Hoog S, et al. Worldwide phylogenetic distributions and population dynamics of the genus Histoplasma. PLoS Negl Trop Dis. 2016;10:e0004732. doi:10.1371/journal.pntd.0004732.CrossRefPubMed

Titel: Amplification of blood smear DNA to confirm disseminated histoplasmosis
verfasst von: T. Dieng
A. Massaly
D. Sow
S. Vellaissamy
K. Sylla
R. C. Tine
Y. Dieng
C. Hennequin
Publikationsdatum: 18.02.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Infection / Ausgabe 5/2017
Print ISSN: 0300-8126
Elektronische ISSN: 1439-0973
DOI: https://doi.org/10.1007/s15010-017-0989-0

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Amplification of blood smear DNA to confirm disseminated histoplasmosis

Abstract

Background

Case Report

Conclusion

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Parodontalbehandlung verbessert Prognose bei Katheterablation

Antihypertensiva schützen auch alte Menschen noch vor Demenz

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Denken Sie bei starker Blutung auch an die Hemmkörper-Hämophilie

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

Background

Case Report

Conclusion

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