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05.12.2016 | Original Paper | Ausgabe 1/2017

Angiogenesis 1/2017

An extracellular proteasome releases endostatin from human collagen XVIII

Zeitschrift:
Angiogenesis > Ausgabe 1/2017
Autoren:
Maria L. V. Reiss-Pistilli, Detlef Schuppan, Madalena M. S. Barroso, Iranaia Assunção-Miranda, Shirley Farias, Letícia Lery, Michael Bauer, Luiz Juliano, Maria A. Juliano, Tatiana Coelho-Sampaio
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s10456-016-9533-6) contains supplementary material, which is available to authorized users.

Abstract

Endostatin is a potent anti-angiogenic and anti-tumor protein capable of regressing tumors without inducing acquired resistance. Since it is a fragment of the parental molecule, collagen XVIII, its endogenous production depends on the activity of a specific proteolytic enzyme. While such an enzyme has been described in mice, a human counterpart has not been identified so far. Here, we searched for this enzyme by using a fluorescence resonance energy transfer peptide containing the cleavage site of human collagen XVIII. We found that the cleavage activity was present in various murine and human tumor cells but not in untransformed cells. It was ascribed to a large protein complex identified as an extracellular form of proteasome 20S. Since circulating proteasome 20S has recently emerged as an important marker of tumor progression, the possibility of proteasomes controlling the production of angiostatic endostatin may inspire the development of new anticancer therapies.

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Zusatzmaterial
Supplementary Figure 1 A protease isolated from human glioblastoma produces endostatin in cultures of human umbilical vein endothelial cells. A protein fraction was isolated from a primary culture of human glioblastoma exactly as done to produce F6 out of EOMA (see Materials and Methods). Such fraction (100 μl) was incubated with a confluent culture of HUVEC in a six-well plate (9.6 cm2) for 2 h at 37°C in growth medium (199 plus 20% FBS). Culture supernatant was analyzed in SDS-PAGE 12% followed by western blot using a polyclonal anti-endostatin antibody (Millipore, AB1878). A band of 20 kDa was detected, whereas such band was strongly reduced when the experiment was carried out in the presence of 10 μM AEBSF. (DOCX 95 kb)
10456_2016_9533_MOESM1_ESM.docx
Literatur
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