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Erschienen in: Clinical Oral Investigations 2/2014

01.03.2014 | Original Article

An in vitro comparison of fluorescence-aided caries excavation and conventional excavation by microhardness testing

verfasst von: Guangyun Lai, Laikuan Zhu, Xiaohui Xu, Karl-Heinz Kunzelmann

Erschienen in: Clinical Oral Investigations | Ausgabe 2/2014

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Abstract

Objective

The aim of this in vitro study was to compare fluorescence-aided caries excavation with conventional excavation based on the Martens and Vickers hardness of dentin at the cavity floor after caries removal.

Materials and methods

In total, 20 extracted human teeth with dentin caries were bisected through the lesion center into two halves, which were assigned to either the fluorescence-aided caries excavation group or the conventional excavation group. After the treatment, embedding, mounting, and polishing, a line of indentations from the dental pulp across the sound dentin to the cavity floor was made on each sample. The data were compared with Student’s t and Mann–Whitney U tests.

Results

The calculated Vickers hardness of the sound dentin was 57 ± 10 kg/mm2 in the fluorescence-aided caries excavation group and 59 ± 8 kg/mm2 in the conventional excavation group, which is consistent with the previous studies. The absolute and relative Martens hardness measurements of the cavity floor were 224 ± 93 N/mm2 and 46 ± 17 %, respectively, in the fluorescence-aided caries excavation group and 412 ± 75 N/mm2 and 81 ± 14 %, respectively, in the conventional excavation group. Based on either the Martens or Vickers hardness, both the absolute and relative microhardness measurements of the cavity floor after fluorescence-aided caries excavation were significantly lower than the values obtained by conventional excavation.

Conclusion

Fluorescence-aided caries excavation showed the tissue-preserving property and was more conservative than the conventional excavation in this in vitro study.
Literatur
1.
Zurück zum Zitat Alfano RR, Yao SS (1981) Human teeth with and without dental caries studied by visible luminescent spectroscopy. J Dent Res 60:120–122PubMedCrossRef Alfano RR, Yao SS (1981) Human teeth with and without dental caries studied by visible luminescent spectroscopy. J Dent Res 60:120–122PubMedCrossRef
2.
Zurück zum Zitat Black GV (1908) The technical procedures in filling teeth. A work on operative dentistry. Medico-Dental Publishing, Chicago Black GV (1908) The technical procedures in filling teeth. A work on operative dentistry. Medico-Dental Publishing, Chicago
3.
Zurück zum Zitat Buchalla W, Lennon AM (2002) Luminescence assisted caries excavation. International application published under the patent cooperation treaty Buchalla W, Lennon AM (2002) Luminescence assisted caries excavation. International application published under the patent cooperation treaty
4.
Zurück zum Zitat Celiberti P, Francescut P, Lussi A (2006) Performance of four dentine excavation methods in deciduous teeth. Caries Res 40:117–123PubMedCrossRef Celiberti P, Francescut P, Lussi A (2006) Performance of four dentine excavation methods in deciduous teeth. Caries Res 40:117–123PubMedCrossRef
5.
Zurück zum Zitat Chuenarrom C, Benjakul P, Daosodsai P (2009) Effect of indentation load and time on knoop and vickers microhardness tests for enamel and dentin. Mat Res 12:473–476CrossRef Chuenarrom C, Benjakul P, Daosodsai P (2009) Effect of indentation load and time on knoop and vickers microhardness tests for enamel and dentin. Mat Res 12:473–476CrossRef
6.
7.
Zurück zum Zitat Fuentes V, Toledano M, Osorio R, Carvalho RM (2003) Microhardness of superficial and deep sound human dentin. J Biomed Mater Res A 66:850–853PubMedCrossRef Fuentes V, Toledano M, Osorio R, Carvalho RM (2003) Microhardness of superficial and deep sound human dentin. J Biomed Mater Res A 66:850–853PubMedCrossRef
8.
Zurück zum Zitat Fusayama T (1979) Two layers of carious dentin: diagnosis and treatment. Oper Dent 4:63–70PubMed Fusayama T (1979) Two layers of carious dentin: diagnosis and treatment. Oper Dent 4:63–70PubMed
9.
Zurück zum Zitat Fusayama T (1993) New concepts in the pathology and treatment of dental caries. In: A simple pain-free adhesive restorative system by minimal reduction and total etching. Ishiyaku EuroAmerica, Inc Fusayama T (1993) New concepts in the pathology and treatment of dental caries. In: A simple pain-free adhesive restorative system by minimal reduction and total etching. Ishiyaku EuroAmerica, Inc
10.
Zurück zum Zitat Fusayama T, Okuse K, Hosoda H (1966) Relationship between hardness, discoloration, and microbial invasion in carious dentin. J Dent Res 45:1033–1046PubMedCrossRef Fusayama T, Okuse K, Hosoda H (1966) Relationship between hardness, discoloration, and microbial invasion in carious dentin. J Dent Res 45:1033–1046PubMedCrossRef
11.
Zurück zum Zitat Lennon AM, Attin T, Buchalla W (2007) Quality of remaining bacteria and cavity size after excavation with FACE, caries detector dye and conventional excavation in vitro. Oper Dent 32:236–241PubMedCrossRef Lennon AM, Attin T, Buchalla W (2007) Quality of remaining bacteria and cavity size after excavation with FACE, caries detector dye and conventional excavation in vitro. Oper Dent 32:236–241PubMedCrossRef
12.
Zurück zum Zitat Lennon AM, Buchalla W, Rassner B, Becker K, Attin T (2006) Efficiency of 4 caries excavation methods compared. Oper Dent 31:551–555PubMedCrossRef Lennon AM, Buchalla W, Rassner B, Becker K, Attin T (2006) Efficiency of 4 caries excavation methods compared. Oper Dent 31:551–555PubMedCrossRef
13.
Zurück zum Zitat Marshall GW, Habelitz S, Gallagher R, Balooch M, Balooch G, Marshall SJ (2001) Nanomechanical properties of hydrated carious human dentin. J Dent Res 80:1768–1771PubMedCrossRef Marshall GW, Habelitz S, Gallagher R, Balooch M, Balooch G, Marshall SJ (2001) Nanomechanical properties of hydrated carious human dentin. J Dent Res 80:1768–1771PubMedCrossRef
14.
Zurück zum Zitat McComb D (2001) Systematic review of conservative operative caries management strategies. J Dent Educ 65:1154–1161PubMed McComb D (2001) Systematic review of conservative operative caries management strategies. J Dent Educ 65:1154–1161PubMed
15.
Zurück zum Zitat Ogawa K, Yamashita Y, Ichijo T, Fusayama T (1983) The ultrastructure and hardness of the transparent layer of human carious dentin. J Dent Res 62:7–10PubMedCrossRef Ogawa K, Yamashita Y, Ichijo T, Fusayama T (1983) The ultrastructure and hardness of the transparent layer of human carious dentin. J Dent Res 62:7–10PubMedCrossRef
16.
Zurück zum Zitat Roberson T, Sturdevant C (2002) Fundamentals in tooth preparation, 4th edn. Sturdevant’s art and science of operative dentistry, Mosby, St. Louis, pp 294–295 Roberson T, Sturdevant C (2002) Fundamentals in tooth preparation, 4th edn. Sturdevant’s art and science of operative dentistry, Mosby, St. Louis, pp 294–295
17.
Zurück zum Zitat Shahdad SA, McCabe JF, Bull S, Rusby S, Wassell RW (2007) Hardness measured with traditional Vickers and Martens hardness methods. Dent Mater 23:1079–1085PubMedCrossRef Shahdad SA, McCabe JF, Bull S, Rusby S, Wassell RW (2007) Hardness measured with traditional Vickers and Martens hardness methods. Dent Mater 23:1079–1085PubMedCrossRef
18.
Zurück zum Zitat Tyas MJ, Anusavice KJ, Frencken JE, Mount GJ (2000) Minimal intervention dentistry—a review. FDI Commission Project 1–97. Int Dent J 50:1–12PubMedCrossRef Tyas MJ, Anusavice KJ, Frencken JE, Mount GJ (2000) Minimal intervention dentistry—a review. FDI Commission Project 1–97. Int Dent J 50:1–12PubMedCrossRef
19.
Zurück zum Zitat Zavgorodniy AV, Rohanizadeh R, Swain MV (2008) Ultrastructure of dentine carious lesions. Arch Oral Biol 53:124–132PubMedCrossRef Zavgorodniy AV, Rohanizadeh R, Swain MV (2008) Ultrastructure of dentine carious lesions. Arch Oral Biol 53:124–132PubMedCrossRef
20.
Zurück zum Zitat Wilde HR, Wehrstedt A (2000) Martens hardness HM—an international accepted designation for “Hardness under Test Force”. Mater Werkst 31:937–940CrossRef Wilde HR, Wehrstedt A (2000) Martens hardness HM—an international accepted designation for “Hardness under Test Force”. Mater Werkst 31:937–940CrossRef
Metadaten
Titel
An in vitro comparison of fluorescence-aided caries excavation and conventional excavation by microhardness testing
verfasst von
Guangyun Lai
Laikuan Zhu
Xiaohui Xu
Karl-Heinz Kunzelmann
Publikationsdatum
01.03.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Oral Investigations / Ausgabe 2/2014
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-013-0999-y

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