nach oben

Journal of Natural Medicines

Erschienen in:

16.06.2017 | Original Paper

An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide

verfasst von: Madan Kumar Paudel, Seiichi Sakamoto, Hiroyuki Tanaka, Satoshi Morimoto

Erschienen in: Journal of Natural Medicines | Ausgabe 4/2017

Einloggen, um Zugang zu erhalten

Abstract

Wogonin glucuronide (wogonin 7-O-β-d-glucuronide, Wgn) is widely recognized as a constituent of Scutellariae radix, which is used in Kampo medicines. Wgn has been used for both pharmacological (antifebrile uses and in detoxification) and research purposes. A recombinant antigen-binding fragment (rFab) and an antigen-binding fragment from a monoclonal antibody (mFab) against Wgn were constructed and used in an indirect competitive enzyme-linked immunosorbent assay (icELISA) in this study. The rFab and mFab against Wgn showed both activity and recognition against Wgn. The developed icELISA was validated as a quantitative analytical method to detect Wgn by testing both its utility and its reliability using multiple concentrations of Wgn from S. radix. This approach provides a more economic method to analyze and purify Kampo medicines.

Kido K, Morinaga O, Shoyama Y, Tanaka H (2008) Quick analysis of baicalin in Scutellariae Radix by enzyme-linked immunosorbent assay using a monoclonal antibody. Talanta 77:346–350CrossRef

Paudel MK, Putalun W, Sritularak B, Morinaga O, Shoyama Y, Tanaka H, Morimoto S (2011) Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin. Anal Chim Acta 701:189–193CrossRef

Paudel MK, Shirota O, Sasaki-Tabata K, Tanaka H, Sekita S, Morimoto S (2013) Development of an enzyme immunoassay using a monoclonal antibody against the psychoactive diterpenoid salvinorin A. J Nat Prod 76:1654–1660CrossRef

Li HB, Jiang Y, Chen F (2004) Separation methods used for Scutellaria baicalensis active components. J Chromatogr B 812:277–290CrossRef

Li CR, Zhang L, Lin G, Zuo Z (2011) Identification and quantification of baicalein, wogonin, oroxylin A and their major glucuronide conjugated metabolites in rat plasma after oral administration of Radix scutellariae product. J Pharm Biomed Anal 54:750–758CrossRef

Paudel MK, Sakamoto S, Huy LV, Tanaka H, Tomofumi M, Akihito T, Morimoto S (2016) Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody. J Immunoassay Immunochem. doi:10.1080/15321819.2016.1273236 CrossRefPubMed

Paudel MK, Sakamoto S, Van Huy L, Tanaka H, Miyamoto T, Morimoto S (2017) The effect of varying the peptide linker length in a single chain variable fragment antibody against wogonin glucuronide. J Biotechnol 251:47–52CrossRef

Wang S, Zheng C, Liu Y, Zheng H, Wang Z (2008) Construction of multiform scFv antibodies using linker peptide. J Genet Genom 35:313–316CrossRef

Bhattaram VA, Graefe U, Kohlert C, Veit M, Derendorf H (2002) Pharmacokinetics and bioavailability of herbal medicinal products. Phytomedicine 9:1–33CrossRef

10.

Kim YH, Jeong DW, Paek IB, Ji HY, Kim YC, Sohn DH, Lee HS (2006) Liquid chromatography with tandem mass spectrometry for the simultaneous determination of baicalein, baicalin, oroxylin A and wogonin in rat plasma. J Chromatogr B 844:261–267CrossRef

11.

Chung HJ, Lim SY, Kim IS, Bu YM, Kim HC, Kim DH, Yoo HH (2012) Simultaneous determination of baicalein, baicalin, wogonin, and wogonoside in rat plasma by LC-MS/MS for studying the pharmacokinetics of the standardized extract of Scutellariae Radix. Bull Korean Chem Soc 33:177–182CrossRef

12.

Zhang L, Xing D, Ding Y, Wang R, Wang X, Du L (2005) A chromatographic method for baicalin quantification in rat thalamus. Biomed Chromatogr 19:494–497CrossRef

13.

Zhang L, Xing D, Wang W, Wang R, Du L (2006) Kinetic difference of baicalin in rat blood and cerebral nuclei after intravenous administration of Scutellariae Radix extract. J Ethnopharmacol 103:120–125CrossRef

14.

Hou YC, Lin SP, Tsai SY, Ko MH, Chang YC, Chao PD (2011) Flavonoid pharmacokinetics and tissue distribution after repeated dosing of the roots of Scutellaria baicalensis in rats. Planta Med 77:455–460CrossRef

15.

Gu X, Jia X, Feng J, Shen B, Huang Y, Geng S, Sun Y, Wang Y, Li Y, Long M (2010) Molecular modeling and affinity determination of scFv antibody: proper linker peptide enhances its activity. Ann Biomed Eng 38:537–549CrossRef

16.

Weiler EW, Zenk MH (1976) Radioimmunoassay for the determination of digoxin and related compounds in Digitalis lanata. Phytochemistry 15:1537–1545CrossRef

17.

Krebber A, Bornhauser S, Burmester J, Honegger A, Willuda J, Bosshard HR, Plückthun A (1997) Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J Immunol Methods 201:35–55CrossRef

18.

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRef

19.

Umetsu M, Tsumoto K, Hara M, Ashish K, Goda S, Adschiri T, Kumagai I (2003) How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment. J Biol Chem 278:8979–8987CrossRef

20.

Miyaichi Y, Tomimori T (1994) Studies on the constituents of Scutellaria species XVI. On the phenol glycosides of the root of Scutellaria baicalensis Georgi. Nat Med 48:215–218

21.

Miyaichi Y, Tomimori T (1995) Studies on the constituents of Scutellaria species XVII. Phenol glycosides of the root of Scutellaria baicalensis Georgi (2). Nat Med 49:350–353

Titel: An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide
verfasst von: Madan Kumar Paudel
Seiichi Sakamoto
Hiroyuki Tanaka
Satoshi Morimoto
Publikationsdatum: 16.06.2017
Verlag: Springer Singapore
Erschienen in: Journal of Natural Medicines / Ausgabe 4/2017
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-017-1100-y

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2017

Fermented Citrus reticulata (ponkan) fruit squeezed draff that contains a large amount of 4′-demethylnobiletin prevents MK801-induced memory impairment

Structures of antimutagenic constituents in the peels of Citrus limon

Antiosteoporotic activity of a syringic acid diet in ovariectomized mice

Nine pairs of megastigmane enantiomers from the leaves of Eucommia ulmoides Oliver

Kuguacin J isolated from bitter melon leaves modulates paclitaxel sensitivity in drug-resistant human ovarian cancer cells

Phytochemical profile and angiotensin I converting enzyme (ACE) inhibitory activity of Limonium michelsonii Lincz