Skip to main content
main-content

01.12.2012 | Primary research | Ausgabe 1/2012 Open Access

Cancer Cell International 1/2012

Analyzing the gene expression profile of pediatric acute myeloid leukemia with real-time PCR arrays

Zeitschrift:
Cancer Cell International > Ausgabe 1/2012
Autoren:
Tao Yan-Fang, Wu Dong, Pang Li, Zhao Wen-Li, Lu Jun, Wang Na, Wang Jian, Feng Xing, Li Yan-Hong, Ni Jian, Pan Jian
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2867-12-40) contains supplementary material, which is available to authorized users.

Competing interests

The authors have no conflicts of interest to disclose.

Authors’ contributions

PJ designed and directed the study. TYF finished the most of the experiments. WJ, FX and LYH, coordinated data collection and quality control, and assisted in the interpretation of results. WD, PL, ZWL, LJ and WN participated in acquiring laboratory data analysis. NJ participated in study design and coordination, data analysis and interpretation and drafted the manuscript. All authors read and approved the final manuscript.

Abstract

Background

The Real-time PCR Array System is the ideal tool for analyzing the expression of a focused panel of genes. In this study, we will analyze the gene expression profile of pediatric acute myeloid leukemia with real-time PCR arrays.

Methods

Real-time PCR array was designed and tested firstly. Then gene expression profile of 11 pediatric AML and 10 normal controls was analyzed with real-time PCR arrays. We analyzed the expression data with MEV (Multi Experiment View) cluster software. Datasets representing genes with altered expression profile derived from cluster analyses were imported into the Ingenuity Pathway Analysis Tool.

Results

We designed and tested 88 real-time PCR primer pairs for a quantitative gene expression analysis of key genes involved in pediatric AML. The gene expression profile of pediatric AML is significantly different from normal control; there are 19 genes up-regulated and 25 genes down-regulated in pediatric AML. To investigate possible biological interactions of differently regulated genes, datasets representing genes with altered expression profile were imported into the Ingenuity Pathway Analysis Tool. The results revealed 12 significant networks. Of these networks, Cellular Development, Cellular Growth and Proliferation, Tumor Morphology was the highest rated network with 36 focus molecules and the significance score of 41. The IPA analysis also groups the differentially expressed genes into biological mechanisms that are related to hematological disease, cell death, cell growth and hematological system development. In the top canonical pathways, p53 and Huntington’s disease signaling came out to be the top two most significant pathways with a p value of 1.5E-8 and2.95E-7, respectively.

Conclusions

The present study demonstrates the gene expression profile of pediatric AML is significantly different from normal control; there are 19 genes up-regulated and 25 genes down-regulated in pediatric AML. We found some genes dyes-regulated in pediatric AML for the first time as FASLG, HDAC4, HDAC7 and some HOX family genes. IPA analysis showed the top important pathways for pediatric AML are p53 and Huntington’s disease signaling. This work may provide new clues of molecular mechanism in pediatric AML.
Zusatzmaterial
Additional file 1: Genes and PCR primers of Real-time PCR array. (XLS 33 KB)
12935_2012_360_MOESM1_ESM.xls
Additional file 2: Summary of IPA analysis. (PDF 540 KB)
12935_2012_360_MOESM2_ESM.pdf
Authors’ original file for figure 1
12935_2012_360_MOESM3_ESM.jpeg
Authors’ original file for figure 2
12935_2012_360_MOESM4_ESM.jpeg
Authors’ original file for figure 3
12935_2012_360_MOESM5_ESM.jpeg
Authors’ original file for figure 4
12935_2012_360_MOESM6_ESM.jpeg
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2012

Cancer Cell International 1/2012 Zur Ausgabe