The online version of this article (doi:10.1186/1477-7827-10-23) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
EH, BB, JK and P-EO designed the experiments and prepared the manuscript. EH and BB participated in the preparation of the animals and tissues. EH and AW performed RNA extraction, cDNA synthesis. EH performed the RIA, the real time quantitative PCR and the analysis of data. All authors have read and approved the final manuscript.
Androgens induce male characters by activating androgen receptors (AR). Previous quantitative studies on AR in fishes have been limited to few tissues and/or a single season/reproductive state. The aim of this investigation was to study the possible role of AR-beta expression levels in the control of male traits in the three-spined stickleback. To that end, AR-beta expression levels in major tissues in breeding and post-breeding male and female sticklebacks were examined.
AR-beta mRNA levels were quantified in ten tissues; eye, liver, axial muscle, heart, brain, intestine, ovary, testis, kidney and pectoral muscle in six breeding and post-breeding males and females using reverse transcription quantitative PCR.
Breeding in contrast to post-breeding males built nests and showed secondary sexual characters (e.g. kidney hypertrophy) and elevated androgen levels. Post-breeding females had lower ovarian weights and testosterone levels than breeding females. AR-beta was expressed in all studied tissues in both sexes and reproductive states with the highest expression in the gonads and in the kidneys. The kidney is an androgen target organ in sticklebacks, from which breeding males produce the protein spiggin, which is used in nest-building. There was also high AR-beta expression in the intestine, an organ that appears to take over hyperosmo-regulation in fresh water when the kidney hypertrophies in mature males and largely loses this function. The only tissue that showed effects of sex or reproductive state on AR-beta mRNA levels was the kidneys, where post-breeding males displayed higher AR-beta mRNA levels than breeding males.
The results indicate that changes in AR-beta mRNA levels play no or little role in changes in androgen dependent traits in the male stickleback.
Hoffmann E, Österman A, Mayer I, Borg B: 11-ketotestosterone is not responsible for the entire testicular effect on male reproductive behaviour in the threespine stickleback. Behaviour. 2008, 145: 509-525. 10.1163/156853908792451548. CrossRef
Jakobsson S, Borg B, Haux C, Hyllner SJ: An 11-ketotestosterone induced kidney-secreted protein: the nest building glue from male three-spined stickleback, Gasterosteus aculeatus. Fish Physiol Biochem. 1999, 20: 79-85. 10.1023/A:1007776016610. CrossRef
Borg B, Antonopoulou E, Andersson E, Carlberg T, Mayer I: Effectiveness of several androgens in stimulating kidney hypertrophy, a secondary sexual character, in castrated male three-spined stickleback, Gasterosteus aculeatus. Canad J Zool. 1993, 71: 2327-2329. 10.1139/z93-326. CrossRef
Hogan NS, Wartman CA, Finley MA, van der Lee JG, van den Heuvel MR: Simultaneous determination of androgenic and estrogenic endpoints in the threespine stickleback ( Gasterosteus aculeatus) using quantitative RT-PCR. Aqua Toxicol. 2009, 90: 269-276. CrossRef
Jones I, Lindberg C, Jakobsson S, Hellqvist A, Hellman U, Borg B, Olsson PE: Molecular cloning and characterization of spiggin: An androgen-regulated extraorganismal adhesive with structural similarities to von Willebrand factor-related proteins. J Biol Chem. 2001, 276: 17857-17863. 10.1074/jbc.M101142200. CrossRefPubMed
Jolly C, Katsiadaki I, Le Belle N, Mayer I, Dufour S: Development of a stickleback kidney cell culture assay for the screening of androgenic and anti-androgenic endocrine disrupters. Aqua Toxicol. 2006, 79: 158-166. 10.1016/j.aquatox.2006.06.005. CrossRef
Björkblom C, Olsson PE, Katsiadaki I, Wiklund T: Estrogen- and androgen-sensitive bioassays based on primary cell and tissue slice cultures from three-spined stickleback ( Gasterosteus aculeatus). Comp Biochem Physiol C. 2007, 146: 431-442.
Wai EH, Hoar W: The secondary sex characters and reproductive behavior of gonadectomized sticklebacks treated with methyl testosterone. Canad J Zool. 1963, 41: 611-628. 10.1139/z63-034. CrossRef
Wen HS, Zhang JR, Chen CF, Jin GX, Shi B, Feng HE: Cloning and expression analysis of male half-smooth tongue sole's ( Cynoglossus semilaevis) androgen receptor gene. Period Ocean Univ China. 2009, 39: 387-391.
Miura C, Miura T, Yamashita M, Yamauchi K, Nagahama Y: Hormonal induction of all stages of spermatogenesis in germ-somatic cell coculture from immature Japanese eel testis. Dev Growth Differ. 1996, 38: 257-262. 10.1046/j.1440-169X.1996.t01-2-00004.x. CrossRef
Andersson E, Mayer I, Borg B: Inhibitory effect of 11-ketoandrostenedione and androstenedione on spermatogenesis in the three-spined stickleback, Gasterosteus aculeatus L. J Fish Biol. 1988, 33: 835-840. 10.1111/j.1095-8649.1988.tb05530.x. CrossRef
Borg B: Seasonal effects of photoperiod and temperature on spermatogenesis and secondary sexual characters in the three-spined stickleback, Gasterosteus aculeatus L. Canad J Zool. 1982, 60: 3377-3386. 10.1139/z82-427. CrossRef
Borg B: Androgens in teleost fishes. Comp Biochem Physiol. 1994, 109C: 219-245.
Borg B, Mayer I: Androgens and behaviour in the three-spined stickleback. Behaviour. 1995, 132: 1025-1035. 10.1163/156853995X00432. CrossRef
Jensen KM, Kahl MD, Makynen EA, Korte JJ, Leino RL, Butterworth BC, Ankley GT: Characterization of responses to the antiandrogen flutamide in a short-term reproduction assay with the fathead minnow. Aqua Toxicol. 2004, 70: 99-110. 10.1016/j.aquatox.2004.06.012. CrossRef
De Ruiter AJH, Hoogeveen YL, Wendelaar-Bonga SE: Ultrastructure of intestinal and gall-bladder epithelium in the teleost Gasterosteus aculeatus L., as related to their osmoregulatory function. Cell Tissue Res. 1985, 240: 191-198. CrossRef
Lundqvist H, Borg B, Berglund I: Androgens impair seawater adaptability in smolting Baltic salmon ( Salmo salar). Canad J Zool. 1989, 67: 1733-1736. 10.1139/z89-248. CrossRef
Borg B: Stimulation of reproductive behaviour by aromatizable and non-aromatizable androgens in the male three-spined stickleback, Gasterosteus aculeatus. Proc V Congr europ Ichthyol Stockholm. 1987, 269-271.
Park CB, Takemura A, Aluru N, Park YJ, Kim BH, Lee C, Lee YD, Moon TW, Vijayan MM: Tissue-specific suppression of estrogen, androgen and glucocorticoid receptor gene expression in feral vitellogenic male Mozambique tilapia. Chemosphere. 2007, 69: 32-40. 10.1016/j.chemosphere.2007.04.072. CrossRefPubMed
Hoffmann E, Borg B: Sex differences in pectoral muscle but not in pectoral fins in the three-spined stickleback Gasterosteus aculeatus. J Fish Biol. 2006, 68: 1451-1459. 10.1111/j.0022-1112.2006.001030.x. CrossRef
- Androgen receptor-beta mRNA levels in different tissues in breeding and post-breeding male and female sticklebacks, Gasterosteus aculeatus
- BioMed Central
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