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07.01.2019 | Original Paper

Andrographolide impairs alpha-naphthylisothiocyanate-induced cholestatic liver injury in vivo

verfasst von: Lei Wang, Fei Cao, Li-li Zhu, Peng Liu, Yu-ru Shang, Wen-hui Liu, Xin Dong, Hai-dong Bao, Peng Gong, Zhong-yu Wang

Erschienen in: Journal of Natural Medicines

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Abstract

To investigate if andrographolide impairs cholestatic liver injury. All rats were randomly divided into six groups—(1) control (n = 6), (2) control + 200 mg/kg andrographolide (n = 6), (3) alpha-naphthylisothiocyanate (ANIT)-control (n = 6), (4) ANIT + 50 mg/kg andrographolide (n = 6), (5) ANIT + 100 mg/kg andrographolide (n = 6), and (6) ANIT + 200 mg/kg andrographolide (n = 6). We gavaged 50 mg/kg ANIT to mimic cholestatic liver injury in rats. Seven days after treatment, all the rats were killed. Serum biochemistry and hepatic histopathological assays were performed to evaluate liver injury. We observed that 200 mg/kg andrographolide significantly decreased the level of alanine transaminase, aspartate aminotransferase, alkaline phosphatase, γ-glutamyltranspeptidase, total bilirubin, and total bile acid in the blood. It also markedly decreased hepatic interleukin-6 and tumor necrosis factor α. Furthermore, 200 mg/kg andrographolide significantly decreased malondialdehyde but increased superoxide dismutase, glutathione, and erythrocyte glutathione peroxidase. Moreover, 200 mg/kg andrographolide effectively increased the accumulation of sirtuin 1 and nuclear erythroid 2-related factor-2. It also attenuated the level of nuclear factor kappa-light-chain-enhancer of activated B and cyclooxygenase-2. These data suggest that andrographolide may impair cholestatic liver injury via anti-inflammatory and anti-oxidative stress.

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Titel: Andrographolide impairs alpha-naphthylisothiocyanate-induced cholestatic liver injury in vivo
verfasst von: Lei Wang
Fei Cao
Li-li Zhu
Peng Liu
Yu-ru Shang
Wen-hui Liu
Xin Dong
Hai-dong Bao
Peng Gong
Zhong-yu Wang
Publikationsdatum: 07.01.2019
Verlag: Springer Singapore
Erschienen in: Journal of Natural Medicines
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-018-01275-3

Springer Medizin

Abstract

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