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28.01.2019 | Original Article

Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment

verfasst von: Priyadarshni Bindal, Nareshwaran Gnanasegaran, Umesh Bindal, Nazmul Haque, Thamil Selvee Ramasamy, Wen Lin Chai, Noor Hayaty Abu Kasim

Erschienen in: Clinical Oral Investigations | Ausgabe 10/2019

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Abstract

Objective

In this study, we aimed to determine the suitable concentrations of human platelet lysate (HPL) and platelet-rich plasma (PRP) for maintaining the in vitro proliferative and angiogenic potential of inflamed dental pulp stem cells.

Materials and methods

Lipopolysaccharide (LPS)-induced inflamed dental pulp-derived stem cells (iDPSCs) were treated with different concentrations of HPL and PRP (10% and 20%) followed by determination of viability using Alamar Blue assay. Expression of angiogenesis-, adhesion-, and inflammation-regulating genes was also analyzed using RT-qPCR array. Furthermore, expression of growth factors at protein level in the cell culture microenvironment was measured using multiplex assay.

Results

Viability of iDPSCs was significantly (p < 0.05) higher in 20% HPL-supplemented media compared to iDPSCs. Expression of 10 out of 12 selected angiogenic genes, four out of seven adhesion molecules, and seven out of nine cytokine-producing genes were significantly (p < 0.05) higher in cells maintained in 20% HPL-supplemented media compared to that in FBS-supplemented media. Furthermore, expression of all the selected growth factors was significantly higher (p < 0.05) in the supernatants from 20% HPL media at 12 and 24 h post-incubation.

Conclusion

This study suggests that 20% HPL could be optimum to stimulate angiogenesis-related factors in iDPSCs while maintaining their viability.

Clinical relevance

This data may suggest the potential use of 20% HPL for expanding DPSCs scheduled for clinical trials for regenerative therapies including dental pulp regeneration.

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Titel: Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment
verfasst von: Priyadarshni Bindal
Nareshwaran Gnanasegaran
Umesh Bindal
Nazmul Haque
Thamil Selvee Ramasamy
Wen Lin Chai
Noor Hayaty Abu Kasim
Publikationsdatum: 28.01.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 10/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-02811-5

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Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

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