Anti-colitic effects of Oldenlandia umbellata L. through NF-κB pathway inhibition in a DSS-induced mouse model
- 23.06.2025
- Original Article
Erschienen in:
- Verfasst von
- X. Janet Bertilla
- S. Rupachandra
- Erschienen in
- Inflammopharmacology | Ausgabe 7/2025
Abstract
Background
Ulcerative colitis (UC) is an inflammatory bowel disease marked by epithelial barrier dysfunction and hyperactivation of immune signaling, particularly in the NF-κB pathway. Conventional therapies are limited by side effects and relapse risk, highlighting the need for safer, plant-based interventions. Oldenlandia umbellata L., a traditional herb, has demonstrated anti-inflammatory potential but remains underexplored in gastrointestinal disorders.
Objective
This study investigates the therapeutic efficacy of a methanolic extract of O.umbellata L. (MEOU) in a dextran sulfate sodium (DSS)-induced mouse model of colitis, focusing on its effects on clinical symptoms, histopathology, intestinal permeability, cytokine expression, and NF-κB signaling.
Methods
Acute colitis was induced in BALB/c mice with 5% DSS for 7 days, followed by oral treatment with MEOU (100, 200 or 400 mg/kg) and prednisolone (5 mg/kg) for another 7 days. Clinical parameters (body weight, Disease Activity Index (DAI), colon and spleen morphology, and histopathology were evaluated. Intestinal permeability was measured by FITC-dextran assay, while TNF-α, IL-6 mRNA levels, and NF-κB p65 protein levels were analyzed by RT-qPCR and Western blot, respectively.
Results
MEOU significantly attenuated weight loss, reduced DAI scores, and reversed colon shortening and splenomegaly in a dose-dependent manner. Histological analysis revealed preserved mucosal structure and reduced inflammatory infiltration. FITC-dextran assays confirmed improved barrier integrity. Molecular analyses showed that MEOU downregulated pro-inflammatory cytokines and suppressed NF-κB p65 activation.
Conclusion
MEOU exhibits potent anti-colitic activity through anti-inflammatory and barrier-protective mechanisms by NF-κB pathway inhibition, supporting its potential as a plant-based therapeutic for UC.
Graphical abstract
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- Titel
- Anti-colitic effects of Oldenlandia umbellata L. through NF-κB pathway inhibition in a DSS-induced mouse model
- Verfasst von
-
X. Janet Bertilla
S. Rupachandra
- Publikationsdatum
- 23.06.2025
- Verlag
- Springer International Publishing
- Erschienen in
-
Inflammopharmacology / Ausgabe 7/2025
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608 - DOI
- https://doi.org/10.1007/s10787-025-01808-9
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