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02.06.2020 | Original Research Article

Anti-inflammatory latex proteins of the medicinal plant Calotropis procera: a promising alternative for oral mucositis treatment

verfasst von: Márcio V. Ramos, Ana Paula F. Freitas, Renata F. C. Leitão, Deiziane V. S. Costa, Gilberto S. Cerqueira, Dainesy S. Martins, Conceição S. Martins, Nylane M. N. Alencar, Larissa Barbosa N. Freitas, Gerly Anne C. Brito

Erschienen in: Inflammation Research | Ausgabe 9/2020

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Abstract

Objective and design

Oral mucositis (OM) is an intense inflammatory reaction progressing to tissue damage and ulceration. The medicinal uses of Calotropis procera are supported by anti-inflammatory capacity. PII-IAA, a highly homogenous cocktail of laticifer proteins (LP) prepared from the latex of C. procera, with recognized pharmacological properties was tested to treat OM.

Materials and subjects

Male Golden Sirius hamsters were used in all treatments.

Treatment

The latex protein samples were injected i.p. (5 mg/Kg) 24 h before mucositis induction (mechanical trauma) and 24 h later.

Methods

Histology, cytokine measurements [ELISA], and macroscopic evaluation [scores] were performed.

Results

PII-IAA eliminated OM, accompanied by total disappearance of myeloperoxidase activity and release of IL-1b, as well as reduced TNF-a. Oxidative stress was relieved by PII-IAA treatment, as revealed by MDA and GSH measurements. PII-IAA also reduced the expression of adhesion molecules (ICAM-1) and Iba-1, two important markers of inflammation, indicating modulatory effects. Histological analyses of the cheek epithelium revealed greater deposition of type I collagen fibers in animals given PII-IAA compared with the control group. This performance was only reached when LPPII was treated with iodoacetamide (IAA), an irreversible inhibitor of proteolytic activity of cysteine proteases. The endogenous proteolytic activity of LPPII induced adverse effects in animals. Candidate proteins involved in the phytomodulatory activity are proposed.

Conclusions

Therapy was successful in treating OM with the laticifer protein fraction, containing peptidases and osmotin, from Calotropis procera. The effective candidate from the latex proteins for therapeutic use is PII-IAA.

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Titel: Anti-inflammatory latex proteins of the medicinal plant Calotropis procera: a promising alternative for oral mucositis treatment
verfasst von: Márcio V. Ramos
Ana Paula F. Freitas
Renata F. C. Leitão
Deiziane V. S. Costa
Gilberto S. Cerqueira
Dainesy S. Martins
Conceição S. Martins
Nylane M. N. Alencar
Larissa Barbosa N. Freitas
Gerly Anne C. Brito
Publikationsdatum: 02.06.2020
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 9/2020
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-020-01365-7

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Anti-inflammatory latex proteins of the medicinal plant Calotropis procera: a promising alternative for oral mucositis treatment

Abstract

Objective and design

Materials and subjects

Treatment

Methods

Results

Conclusions

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Abstract

Objective and design

Materials and subjects

Treatment

Methods

Results

Conclusions

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