The online version of this article (doi:10.1186/1475-2875-11-34) contains supplementary material, which is available to authorized users.
Donald L Gardiner and Katharine R Trenholme contributed equally to this work.
The authors declare that they have no competing interests.
DLG, KRT and DL conceived the study, DLG, KRT and CLP designed the study, undertook the experimental procedures and data analysis. All authors participated in interpretation of the data. KRT drafted the final manuscript, which was read and approved by all authors.
Recent renewed emphasis on the eradication of malaria has highlighted the need for more tools with which to achieve this ambitious goal. One high priority area is the need to determine the gametocytocidal activity of both currently used anti-malarial drugs and those in the development pipeline. However, testing the activity of compounds against Plasmodium falciparum gametocytes is technically challenging both in vivo and in vitro.
Here the use of a simple robust assay to screen a panel of currently used and experimental anti-malarial drugs against mature P. falciparum gametocytes is described.
Eight of 44 compounds tested reduced gametocyte viability by at least 50% and three showed IC50 values in nM range.
There is a need to identify new compounds with activity against late stage gametocytes and the information provided by this in vitro assay is a valuable first step, which can guide future clinical studies.
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- Anti-malarial drugs: how effective are they against Plasmodium falciparum gametocytes?
Christopher L Peatey
Donald L Gardiner
Katharine R Trenholme
- BioMed Central
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