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Erschienen in: Autoimmunity Highlights 1-2/2015

Open Access 01.08.2015 | Original Article

Anti-zinc transporter protein 8 autoantibodies significantly improve the diagnostic approach to type 1 diabetes: an Italian multicentre study on paediatric patients

verfasst von: Martina Fabris, Silvia Zago, Marco Liguori, Maria Teresa Trevisan, Manuela Zanatta, Alberto Comici, Giorgio Zanette, Eva Carlin, Francesco Curcio, Elio Tonutti

Erschienen in: Autoimmunity Highlights | Ausgabe 1-2/2015

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Abstract

Background and aim

Anti-ZnT8 antibodies (ZnT8A) were recently proposed as a new independent serological marker in Type 1 diabetes (T1D), leading to a significant improvement of the positive predictive value of autoantibody measurement in this setting. The aim of this retrospective multicentre study was to investigate ZnT8A as a complement to the current T1D autoantibody assays in a large cohort of paediatric Italian patients.

Methods

ZnT8A were assessed by ELISA in 213 T1DM paediatric patients referred to six different centres in North-East Italy. Fifty-four were analysed at disease onset, 79 within 4 years from diagnosis and 80 after 5 or more years from diagnosis. Retrospective data about islet cell autoantibodies (ICA), anti-insulin (IAA), anti-glutamate decarboxylase (GADA) and anti-protein tyrosine phosphatase IA-2 (IA-2A) antibodies were collected and compared.

Results

Overall, ZnT8A showed positive results in 106/213 (49.8 %) T1D patients and were found in 10 (4.7 %) subjects previously classified as autoantibody negative based on the existing markers (GADA, IA-2A, IAA and ICA), increasing the overall diagnostic sensitivity from 85.9 to 90.6 %. ZnT8A disclosed the same sensitivity (61.1 %) at disease onset as GADA (61.1 %) and higher than IA-2A (53.7 %), with only GADA showing much persistence in the long-term follow-up. Focusing on patients at disease onset, all the ICA positive were associated with at least one positive autoantibody among GADA, IA-2A and ZnT8A, 16.7 % of whom presenting only anti-ZnT8-positive antibodies.

Conclusion

This study confirms ZnT8A as an important additional and independent diagnostic marker of T1D and supports its introduction in the routine diagnostic process to replace less sensitive methods and improve the overall autoantibody sensitivity.
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Metadaten
Titel
Anti-zinc transporter protein 8 autoantibodies significantly improve the diagnostic approach to type 1 diabetes: an Italian multicentre study on paediatric patients
verfasst von
Martina Fabris
Silvia Zago
Marco Liguori
Maria Teresa Trevisan
Manuela Zanatta
Alberto Comici
Giorgio Zanette
Eva Carlin
Francesco Curcio
Elio Tonutti
Publikationsdatum
01.08.2015
Verlag
Springer International Publishing
Erschienen in
Autoimmunity Highlights / Ausgabe 1-2/2015
Print ISSN: 2038-0305
Elektronische ISSN: 2038-3274
DOI
https://doi.org/10.1007/s13317-015-0068-4

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