The authors declare that they have no competing interests.
AD: made contribution to the study algogenic activities. HBM: Was responsible for the conception and design, testing and data acquisition, analysis and data interpretation and drafted the manuscript. Audrey C-L: made contribution to convulsing activity. MD made contribution to preparation of crude extract and its fractions of the defensive secretion from the Mediterranean sponge, Spongiaofficinalis Monia Deghrigue. AB made contribution to convulsing activity and statistical analysis. All authors read and approved the final manuscript.
This study progresses in the direction of identifying component(s) from the Mediterranean sponge, Spongia officinalis with anticonvulsant and analgesic activities. We investigated the efficacy of crude extract and its semi-purified fractions (F1-F3) of the defensive secretion from Spongia officinalis for their in vivo anticonvulsant activity using the pentylenetetrazole (PTZ) seizure model and analgesic activity using the writhing test in mice. Among the series the crude extract exhibited interesting analgesic activity in a dose dependent manner. Similarly the fraction F2 showed a partial protection of mice from PTZ-induced seizure and interesting analgesic activity in a dose dependent manner. The purification and the determination of chemical structure(s) of compound(s) of this active fraction are under investigation.