Background
Methods
Animals
Overall research design
Chronic unpredictable mild stress procedure
Intracerebroventricular injection
Acupuncture and drug treatment
Behavior tests
Western blotting analysis
Statistical analysis
Results
Changes in sucrose intake
Group | Sucrose intake (g) | |
---|---|---|
0 d | 21 d | |
Control | 25.96 ± 2.83 | 38.31 ± 6.63 |
CUMS | 27.44 ± 3.68 | 22.17 ± 5.96★★
|
Acu | 25.20 ± 2.77 | 28.49 ± 3.47★★▲▲
|
FLX | 27.47 ± 4.29 | 33.51 ± 5.17★▲▲
|
DMSO | 27.37 ± 3.95 | 18.35 ± 2.54 |
PD | 27.77 ± 4.13 | 17.43 ± 3.96▲
|
Acu+PD | 26.03 ± 3.02 | 18.15 ± 4.38◆◆
|
FLX+PD | 25.37 ± 3.31 | 21.06 ± 5.10■■
|
Comparison of crossing and rearing numbers
Group | Crossing number | Rearing number | ||
---|---|---|---|---|
0 d | 21 d | 0 d | 21 d | |
Control | 66.2 ± 20.0 | 36.9 ± 7.8 | 18.4 ± 5.9 | 15.0 ± 5.2 |
CUMS | 73.3 ± 17.6 | 4.7 ± 2.1★★
| 13.5 ± 2.3 | 2.5 ± 1.7★★
|
Acu | 64.4 ± 18.0 | 15.0 ± 5.1★★▲▲
| 14.8 ± 3.7 | 8.9 ± 2.5▲▲
|
FLX | 68.5 ± 19.1 | 16.9 ± 6.0★★▲▲
| 15.9 ± 3.8 | 9.5 ± 2.1▲▲
|
DMSO | 64.9 ± 18.6 | 3.8 ± 2.3 | 14.3 ± 3.6 | 1.5 ± 1.4 |
PD | 62.2 ± 18.8 | 4.1 ± 2.9 | 12.8 ± 3.0 | 1.4 ± 1.3 |
Acu+PD | 69.0 ± 18.0 | 17.0 ± 5.5 | 14.3 ± 3.1 | 4.2 ± 2.0◆◆
|
FLX+PD | 60.4 ± 20.8 | 18.7 ± 7.2 | 13.4 ± 3.9 | 3.7 ± 2.2■■
|
Western blot analysis of ERK1/2 and p-ERK1/2 in the hippocampus
Group | ERK1/2/β-actin | p-ERK1/2/β-actin |
---|---|---|
Control | 0.573 ± 0.101 | 0.350 ± 0.040 |
CUMS | 0.512 ± 0.108 | 0.264 ± 0.041★★
|
Acu | 0.515 ± 0.073 | 0.332 ± 0.046▲
|
FLX | 0.546 ± 0.104 | 0.333 ± 0.036▲
|
DMSO | 0.505 ± 0.107 | 0.301 ± 0.045 |
PD | 0.508 ± 0.089 | 0.185 ± 0.069▲▼▼
|
Acu+PD | 0.502 ± 0.059 | 0.255 ± 0.060◆
|
FLX+PD | 0.480 ± 0.077 | 0.278 ± 0.059 |
Western blot analysis of CREB and p-CREB in the hippocampus
Group | CREB/β-actin | p-CREB/β-actin |
---|---|---|
Control | 0.434 ± 0.045 | 0.367 ± 0.053 |
CUMS | 0.371 ± 0.039★
| 0.261 ± 0.092★★
|
Acu | 0.427 ± 0.037▲
| 0.344 ± 0.042▲
|
FLX | 0.423 ± 0.055▲
| 0.345 ± 0.036▲
|
DMSO | 0.394 ± 0.022 | 0.259 ± 0.061 |
PD | 0.419 ± 0.030 | 0.198 ± 0.072 |
Acu+PD | 0.371 ± 0.041◆
| 0.270 ± 0.063◆
|
FLX+PD | 0.345 ± 0.065■■
| 0.272 ± 0.043■
|
Western blot analysis of BDNF in the hippocampus
Group | BDNF/β-actin |
---|---|
Control | 0.334 ± 0.076 |
CUMS | 0.217 ± 0.018★★
|
Acu | 0.301 ± 0.066▲
|
FLX | 0.319 ± 0.069▲
|
DMSO | 0.222 ± 0.043 |
PD | 0.231 ± 0.054 |
Acu+PD | 0.245 ± 0.088 |
FLX+PD | 0.250 ± 0.090 |